FlyBase Allele Report: Dmel\S[hs.PK]

General Information

Symbol

Dmel\S^hs.PK

Species

D. melanogaster

Name

heat shock construct of Kolodkin

FlyBase ID

FBal0039011

Feature type

allele

Associated gene

Dmel\S

Associated Insertion(s)

Carried in Construct

P{hsp70-S}

Key Links

Transgenic product class

cDNA

(Kolodkin et al., 1994)

wild_type

(Kolodkin et al., 1994)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Kolodkin et al., 1994)

wild_type

(Kolodkin et al., 1994)

Mutagen

in vitro construct

(Kolodkin et al., 1994)

Progenitor genotype

Carried in construct

P{hsp70-S}

Cytology

Description

A S cDNA containing the entire open reading frame is expressed from an Hsp70 promoter.

(Kolodkin et al., 1994)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Overexpression of S^hs.PK throughout larval life and pupariation does not induce any wing phenotypes.

(Guichard et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

S^hs.PK, rho^hs.PSt has visible phenotype, enhanceable by spi^hs.PG

(Guichard et al., 1999)

Enhancer of

Statement

Reference

S^hs.PK is an enhancer of visible phenotype of rho^Wk

(Guichard et al., 1999)

Phenotype Manifest In

Enhanced by

Statement

Reference

S^hs.PK, rho^hs.PSt has wing phenotype, enhanceable by spi^hs.PG

(Guichard et al., 1999)

S^hs.PK has wing phenotype, enhanceable by rho^hs.PSt

(Guichard et al., 1999)

NOT Enhanced by

Statement

Reference

S^hs.PK has phenotype, non-enhanceable by spi^hs.PG

(Guichard et al., 1999)

Enhancer of

Statement

Reference

S^hs.PK is an enhancer of wing phenotype of rho^Wk

(Guichard et al., 1999)

S^hs.PK is an enhancer of wing vein phenotype of rho^Wk

(Guichard et al., 1999)

NOT Enhancer of

Statement

Reference

S^hs.PK is a non-enhancer of phenotype of spi^hs.PG

(Guichard et al., 1999)

Other

Statement

Reference

S^hs.PK, rho^hs.PSt, spi^hs.PG has wing vein phenotype

(Guichard et al., 1999)

S^hs.PK, rho^hs.PSt, spi^hs.PG has eye | anterior phenotype

(Guichard et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

rho^Wk; S^hs.PK flies heatshocked between 6 and 9 hours after puparium formation (APF) show an extra vein phenotype much stronger than either rho^Wk or S^hs.PK alone. Entire intervein sectors are converted into veins. Flies heatshocked between 9 and 12 hours APF showed a moderate extra vein phenotype, consisting of ectopic crossveins and additional longitudinal veins. Between 12 and 24 hours APF heatshocking induced diffuse extra vein material between wing veins L1 and L2, and between L4 and L5. When S^hs.PK flies that also carry spi^hs.PG are exposed to heatshock during larval or early pupal stages no phenotype is seen. When S^hs.PK; rho^hs.PSt; spi^hs.PG are exposed to during early pupariation (mostly between 6 and 12 hours APF) a phenotype is seen that is stronger than S^hs.PK; rho^hs.PSt alone. Also, in contrast to the diffuse ectopic veins induced by S^hs.PK; rho^hs.PSt, a sharp pattern of well defined forked longitudinal vein is observed. A phenotype can also be seen in the eye, in which cell death is localised at the anterior part of the eye, when heat shock is provided between 0 and 6 hours APF.

(Guichard et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Used to generate S^hs.8 and S^hs.7.

(Kolodkin et al., 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

S^hs.PK

Name Synonyms

heat shock construct of Kolodkin

(Kolodkin et al., 1994)

Secondary FlyBase IDs

References (2)

Report Sections

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