FlyBase Allele Report: Dmel\rho[hs.PSt]

Expression of rho^hs.PSt using multiple heat shocks during larval stages leads to failure of photoreceptor differentiation and a dorsoventral stripe of scar tissue in the adult eye.

(Chen and Chien, 1999)

Embryos overexpressing rho^hs.PSt show a 'cyclops' phenotype in which the optic lobes are enlarged and show dorsal fusion.

(Daniel et al., 1999)

Heat shock driven misexpression of rho^hs.PSt produces a number of wing phenotypes including, blisters separating the dorsal and ventral surfaces of the wing, ectopic crossveins, an ectopic vein spur running between wing veins L3 and L4 near the margin, and a thickened L3 vein. All phenotypes are seen if heatshock occurs within 0-30 hours after puparium formation (APF).

(Guichard et al., 1999)

Ubiquitous and sustained expression of rho^hs.PSt under heatshock, results in supernumerary midline glial cells.

(Lanoue and Jacobs, 1999)

Overexpression of rho^hs.PSt results in dramatically enlarged wing vein tissue.

(Martin-Blanco et al., 1999)

Causes complex deformity of the embryonic visual system. Optic lobe is enlarged and invaginates earlier and at a more dorsomedial position than in wild type. The differentiation of Bolwig's organ is also abnormal; in many cases it does not separate from the optic lobe and does not form axons. Heat shock activation between 5 and 7 hours postfertilization has the strongest effect.

(Dumstrei et al., 1998)

Shows extra wing vein material phenotype, even at 25^oC.

(Roch et al., 1998)

Causes dorsalization of the egg.

(Wasserman and Freeman, 1998)

Heat shocked embryos show an increase of 1 or 2 chordotonal organs in the lateral pentascolopidial chordotonal organs (LCh5) and in the ventral cluster.

(Okabe and Okano, 1997)

The number of midline glia cells is increased to an average of 7 cells per segment in homozygous embryos.

(Stemerdink and Jacobs, 1997)

Ectopic expression of rho^hs.PSt in the disc and pupal wing results in the differentiation of ectopic and thicker veins.

(de Celis, 1997)

Heat induced expression causes an increase in the number of chordotonal neurons.

(zur Lage et al., 1997)

The number of MGA and MGM cells can be increased by the ectopic expression of rho. Onset of midline glial cell death is shifted earlier, to stage 12 rather than stage 13, in these embryos.

(Sonnenfeld and Jacobs, 1995)

Heat shock at germband extension leads to the production of stretch receptor organ precursor cells and heat inductions during wing vein development leads to the formation of ectopic veins.

(Sturtevant et al., 1994)

Eggs laid after heat shock have dorsalized eggshells and give rise to dorsalized embryos, with expansion of dorsal structures at the expense of ventral. In some embryos stronger dorsalization was detected in the anterior region than the posterior, similar to the mutant phenotype in fs(1)K10 mutants. The large percentage of nonhatching embryos reflects failure of fertilization and dorsalization of the embryo.

(Ruohola-Baker et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

rho^hs.PSt has visible phenotype, enhanceable by ed[+]/ed^1X5

(Bai et al., 2001)

S^hs.PK, rho^hs.PSt has visible phenotype, enhanceable by spi^hs.PG

(Guichard et al., 1999)

Other

Statement

Reference

Scer\GAL4^en-e16E, msk^UAS.cLa, rho^hs.PSt has abnormal size | adult stage phenotype

(Marenda et al., 2006)

emc¹, rho^hs.PSt has visible phenotype

(Milan et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

rho^hs.PSt has eye phenotype, enhanceable by ed[+]/ed^1X5

(Bai et al., 2001)

S^hs.PK, rho^hs.PSt has wing phenotype, enhanceable by spi^hs.PG

(Guichard et al., 1999)

rho^hs.PSt has phenotype, enhanceable by bs⁰³²⁶⁷

(Roch et al., 1998)

rho^hs.PSt has wing vein phenotype, enhanceable by tkv¹/tkv⁸

(de Celis, 1997)

rho^hs.PSt has wing vein | ectopic phenotype, enhanceable by bs^ba

(Sturtevant and Bier, 1995)

rho^hs.PSt has wing vein | ectopic phenotype, enhanceable by bs¹¹

(Sturtevant and Bier, 1995)

rho^hs.PSt has wing vein | ectopic phenotype, enhanceable by bs²

(Sturtevant and Bier, 1995)

rho^hs.PSt has phenotype, enhanceable by bs^Px

(Sturtevant and Bier, 1995)

NOT Enhanced by

Statement

Reference

rho^hs.PSt has phenotype, non-enhanceable by spi^hs.PG

(Guichard et al., 1999)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, rho^hs.PSt has wing vein | ectopic phenotype, suppressible by Scer\GAL4^en-e16E/msk^UAS.cLa

(Marenda et al., 2006)

rho^hs.PSt has dorsal appendage phenotype, suppressible by spi¹

(Wasserman and Freeman, 1998)

rho^hs.PSt has egg phenotype, suppressible by spi¹

(Wasserman and Freeman, 1998)

rho^hs.PSt has wing vein | ectopic phenotype, suppressible by dpp^s4/dpp^s8

(de Celis, 1997)

NOT suppressed by

Statement

Reference

rho^hs.PSt has phenotype, non-suppressible by spi^hs.PG

(Guichard et al., 1999)

Enhancer of

Statement

Reference

rho^hs.PSt is an enhancer of adult optic lobe | adult stage phenotype of vap²

(Botella et al., 2003)

rho^hs.PSt is an enhancer of wing phenotype of S^hs.PK

(Guichard et al., 1999)

NOT Enhancer of

Statement

Reference

rho^hs.PSt is a non-enhancer of phenotype of Egfr^flb-2E07

(Lanoue and Jacobs, 1999)

rho^hs.PSt is a non-enhancer of phenotype of S^IIN

(Lanoue and Jacobs, 1999)

Suppressor of

Statement

Reference

rho^hs.PSt is a suppressor of midline glial cell phenotype of spi¹

(Lanoue and Jacobs, 1999)

NOT Suppressor of

Statement

Reference

rho^hs.PSt is a non-suppressor of phenotype of Egfr^flb-2E07

(Lanoue and Jacobs, 1999)

rho^hs.PSt is a non-suppressor of phenotype of S^IIN

(Lanoue and Jacobs, 1999)

Other

Statement

Reference

Scer\GAL4^en-e16E, msk^UAS.cLa, rho^hs.PSt has wing phenotype

(Marenda et al., 2006)

S^hs.PK, rho^hs.PSt, spi^hs.PG has eye | anterior phenotype

(Guichard et al., 1999)

S^hs.PK, rho^hs.PSt, spi^hs.PG has wing vein phenotype

(Guichard et al., 1999)

rho^hs.PSt, spi¹ has larval longitudinal connective phenotype

(Lanoue and Jacobs, 1999)

emc¹, rho^hs.PSt has wing vein | ectopic phenotype

(Milan et al., 1997)

emc¹, rho^hs.PSt has wing vein phenotype

(Milan et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The extra veins in the adult wing induced by rho^hs.PSt are strongly suppressed by the addition of ectopic msk^Scer\UAS.cLa (under the control of Scer\GAL4^en-e16E). rho^hs.PSt msk^Scer\UAS.cLa (under the control of Scer\GAL4^en-e16E) wings are significantly larger than rho^hs.PSt wings, despite no decrease in cell density, indicating an increase in cell division.

(Marenda et al., 2006)

When rho^hs.PSt; spi^hs.PG flies are exposed to heat shock, no effect of elevated spi^hs.PG expression is seen beyond that attributable to rho^hs.PSt misexpression. When S^hs.PK; rho^hs.PSt; spi^hs.PG are exposed to during early pupariation (mostly between 6 and 12 hours after puparium formation (APF)) a phenotype is seen that is stronger than S^hs.PK; rho^hs.PSt alone. Also, in contrast to the diffuse ectopic veins induced by S^hs.PK ; rho^hs.PSt, a sharp pattern of well defined forked longitudinal veins is observed. A phenotype can also be seen in the eye, in which cell death is localised at the anterior part of the eye, when heat shock is provided between 0 and 6 hours APF.

(Guichard et al., 1999)

The ubiquitous expression of rho^hs.PSt under heatshock leads to a partial suppression of the spi¹ loss of midline glial cell phenotype. No significant suppression of the axon tract phenotype is seen, however longitudinal tracts are thinner in these embryos. (this effect is not seen when rho^hs.PSt is added to wild-type embryos). Commissure separation in rho^hs.PSt,spi¹ is not improved. The ubiquitous expression of rho^hs.PSt under heatshock has no effect on S^IIN or Egfr^flb-2E07 embryos.

(Lanoue and Jacobs, 1999)

Dorsalization of the egg caused by rho^hs.PSt is suppressed by halving the dose of spi.

(Wasserman and Freeman, 1998)

Homozygous emc¹ clones carrying rho^hs.PSt differentiate extra veins and thicker veins. Clones located in both surfaces of the wing have a stronger phenotype than those located in only one surface.

(Milan et al., 1997)

The differentiation of thicker veins seen in rho^hs.PSt is strongly increased in combination with tkv¹/tkv⁸, while the rho^hs.PSt ectopic vein phenotype is suppressed in combination with dpp^s4/dpp^s8.

(de Celis, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

rho^hs.PSt partially rescues rho^PΔ38

(Lanoue and Jacobs, 1999)

Comments

The ubiquitous expression of rho^hs.PSt under heatshock is sufficient to suppress the loss of midline glial cell seen in rho^PΔ38 embryos to wild-type. Commissure separation in rho^hs.PSt,rho^PΔ38 is not improved.

(Lanoue and Jacobs, 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Generated by Sturtevant, Roark and Bier.

(Ruohola-Baker et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Hs-rho30A

(Roch et al., 1998)

rho^hs.PSt

ve^hs.IC

ve^hs.PS

ve^hs.PSt

Name Synonyms

heat shock construct of Sturtevant

Secondary FlyBase IDs

FBal0092454

References (29)

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