FlyBase Allele Report: Dmel\spi[hs.PG]

General Information

Symbol

Dmel\spi^hs.PG

Species

D. melanogaster

Name

heat shock construct of Guichard

FlyBase ID

FBal0098650

Feature type

allele

Associated gene

Dmel\spi

Associated Insertion(s)

Carried in Construct

P{hs-spi.m}

Key Links

Transgenic product class

cDNA

(Guichard et al., 1999)

wild_type

(Guichard et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Guichard et al., 1999)

wild_type

(Guichard et al., 1999)

Mutagen

in vitro construct

(Guichard et al., 1999)

Progenitor genotype

Carried in construct

P{hs-spi.m}

Cytology

Description

Construct: Full length spi cDNA under the control of a heat shock promoter.

(Guichard et al., 1999)

Allele components

Component

Use(s)

Encoded product / tool

spi

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Heat shock induction of spi^hs.PG throughout larval life and pupariation has no detectable phenotype in the wing.

(Guichard et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

spi^hs.PG is an enhancer of visible phenotype of S^hs.PK, rho^hs.PSt

(Guichard et al., 1999)

NOT Enhancer of

Statement

Reference

spi^hs.PG is a non-enhancer of visible phenotype of S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

NOT Suppressor of

Statement

Reference

spi^hs.PG is a non-suppressor of visible phenotype of S^UAS.cGb, Scer\GAL4^Bx-MS1096

(Guichard et al., 1999)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

spi^hs.PG has phenotype, non-enhanceable by S^hs.PK

(Guichard et al., 1999)

Enhancer of

Statement

Reference

spi^hs.PG is an enhancer of wing phenotype of S^hs.PK, rho^hs.PSt

(Guichard et al., 1999)

NOT Enhancer of

Statement

Reference

spi^hs.PG is a non-enhancer of phenotype of rho^hs.PSt

(Guichard et al., 1999)

spi^hs.PG is a non-enhancer of phenotype of S^hs.PK

(Guichard et al., 1999)

NOT Suppressor of

Statement

Reference

spi^hs.PG is a non-suppressor of phenotype of rho^hs.PSt

(Guichard et al., 1999)

Other

Statement

Reference

S^hs.PK, rho^hs.PSt, spi^hs.PG has wing vein phenotype

(Guichard et al., 1999)

S^hs.PK, rho^hs.PSt, spi^hs.PG has eye | anterior phenotype

(Guichard et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

When rho^hs.PSt; spi^hs.PG flies are exposed to heat shock, no effect of elevated spi^hs.PG expression is seen beyond that attributable to rho^hs.PSt. When spi^hs.PG; S^hs.PK flies are exposed to heatshock during larval or early pupal stages no phenotype is seen. Expression of S^Scer\UAS.cGb driven by Scer\GAL4^Bx-MS1096, in a spi^hs.PG background exposed to heatshock, has no observable effect. When S^hs.PK; rho^hs.PSt; spi^hs.PG are exposed to heatshock during early pupariation (mostly between 6 and 12 hours APF) a phenotype is seen that is stronger than S^hs.PK; rho^hs.PSt alone. Also, in contrast to the diffuse ectopic veins induced by S^hs.PK; rho^hs.PSt, a sharp pattern of well defined forked longitudinal vein is observed. A phenotype can also be seen in the eye, in which cell death is localised at the anterior part of the eye, when heat shock is provided between 0 and 6 hours APF.

(Guichard et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

spi^hs.PG partially rescues spi¹/spi³

(Guichard et al., 1999)

Comments

Heat shock expression of spi^hs.PG in spi¹/spi³ mutants can partially rescue lethality and reduces the severity of the spi¹/spi³ mutant phenotype in remaining dead embryos.

(Guichard et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

spi^hs.PG

Name Synonyms

heat shock construct of Guichard

(Guichard et al., 1999)

Secondary FlyBase IDs

References (1)

Report Sections

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