FlyBase Allele Report: Dmel\msk[UAS.cLa]

General Information

Symbol

Dmel\msk^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Lorenzen

FlyBase ID

FBal0122947

Feature type

allele

Associated gene

Dmel\msk

Associated Insertion(s)

Carried in Construct

P{UAS-msk.L}

Also Known As

UAS-msk, UAS:msk

Key Links

Transgenic product class

cDNA

(Lorenzen et al., 2001)

wild_type

(Lorenzen et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Lorenzen et al., 2001)

wild_type

(Lorenzen et al., 2001)

Mutagen

in vitro construct

(Lorenzen et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-msk.L}

Cytology

Description

Expression of a full length msk cDNA is governed by UASt regulatory sequences.

(Lorenzen et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

msk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^en-e16E

(Marenda et al., 2006)

crossvein, with Scer\GAL4^en-e16E

(Baker et al., 2002)

eye | heat sensitive, with Scer\GAL4^GMR.PF

(Vrailas et al., 2006)

eye photoreceptor cell | heat sensitive, with Scer\GAL4^GMR.PF

(Vrailas et al., 2006)

pigment cell | heat sensitive, with Scer\GAL4^GMR.PF

(Vrailas et al., 2006)

posterior crossvein, with Scer\GAL4^en-e16E

(Marenda et al., 2006)

visceral muscle fiber | embryonic stage, with Scer\GAL4^Mef2.PR

(Liu et al., 2013)

visceral muscle fiber | embryonic stage, with Scer\GAL4^twi.PU

(Liu et al., 2013)

wing, with Scer\GAL4^en-e16E

(Marenda et al., 2006, Baker et al., 2002)

wing disc posterior compartment | larval stage, with Scer\GAL4^en-e16E

(Marenda et al., 2006)

wing vein L4, with Scer\GAL4^en-e16E

(Marenda et al., 2006)

wing vein L5, with Scer\GAL4^en-e16E

(Marenda et al., 2006)

Detailed Description

Statement

Reference

Scer\GAL4^twi.PU-mediated expression of msk^Scer\UAS.cLa perturbs the ability of embryonic muscles to firmly adhere to the tendon cells.

Scer\GAL4^Mef2.PR-mediated expression of msk^Scer\UAS.cLa results in detached muscles in embryos.

(Liu et al., 2013)

Expression of msk^Scer\UAS.cLa, under the control of Scer\GAL4^en-e16E in the posterior compartment boundary of the wing disrupts the anteroposterior compartment boundary. Expression of msk^Scer\UAS.cLa, under the control of Scer\GAL4^en-e16E results in the fraction of cells in S-phase increasing compared to wild-type (27% compared to 16% for the control), as does the fraction of cells in G2/M (37% versus 32%), at the expense of cells in G1. Prolonged and elevated expression of msk^Scer\UAS.cLa, under the control of Scer\GAL4^en-e16E results in severe disruption to the adult wing, with nearly normal anterior compartments and severely reduced posteriors due to caspase-dependent cell death (authors term this the `Twiggy wing'). These wings display loss of posterior tissue, including distal regions of veins L4 and L5, and fused posterior and anterior crossveins.

(Marenda et al., 2006)

Expression of msk^Scer\UAS.cLa, under the control of Scer\GAL4^GMR.PF at 18^oC causes a roughening of the eye (a disruption of the facet pattern) and a loss of some red pigment; this is accentuated at 25^oC (over the effect of Scer\GAL4^GMR.PF alone). Reduced numbers of both photoreceptors and pigment cells occurs before the 60 hour pupal stage. Elevating the temperature to 25^oC kills almost all retinal cells before the adult stage, but the cells are not yet lost in the 60 hour pupa.

(Vrailas et al., 2006)

When msk^Scer\UAS.cLa is expressed under the control of Scer\GAL4^en-e16E, homozygous msk⁵ clones induced in the posterior wing can grow to a typical size of more than 100 cells, whereas homozygous msk⁵ clones induced in the anterior wing are typically composed of 8 cells or less, indicating that msk is required for the growth of cells in the wing. Expression of msk^Scer\UAS.cLa under the control of Scer\GAL4^en-e16E results in the cross veins moving closer together than normal, so that they can fuse as one vein with high levels of msk^Scer\UAS.cLa expression. A small number of wing blisters are also seen.

(Baker et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, msk^UAS.cLa has increased cell death phenotype, suppressible by rl^10a

(Marenda et al., 2006)

Scer\GAL4^en-e16E, msk^UAS.cLa has abnormal size | larval stage phenotype, suppressible by rl[+]/rl^10a

(Marenda et al., 2006)

Other

Statement

Reference

Scer\GAL4^en-e16E, msk^UAS.cLa, rho^hs.PSt has abnormal size | adult stage phenotype

(Marenda et al., 2006)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, msk^UAS.cLa has abnormal size | larval stage phenotype

(Marenda et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^twi.PU, msk^UAS.cLa has visceral muscle fiber | embryonic stage phenotype, suppressible by mbc^D11.2/mbc[+]

(Liu et al., 2013)

Scer\GAL4^en-e16E, msk^UAS.cLa has anterior crossvein phenotype, suppressible by rl^10a

(Marenda et al., 2006)

Scer\GAL4^en-e16E, msk^UAS.cLa has posterior crossvein phenotype, suppressible by rl^10a

(Marenda et al., 2006)

Scer\GAL4^en-e16E, msk^UAS.cLa has wing vein L4 phenotype, suppressible by rl^10a

(Marenda et al., 2006)

Scer\GAL4^en-e16E, msk^UAS.cLa has wing disc posterior compartment | larval stage phenotype, suppressible by rl[+]/rl^10a

(Marenda et al., 2006)

msk^UAS.cLa has wing phenotype, suppressible by Scer\GAL4^en-e16E/rl^10a

(Marenda et al., 2006)

Enhancer of

Statement

Reference

Scer\GAL4^en-e16E/msk^UAS.cLa is an enhancer of wing vein L4 phenotype of Scer\GAL4^en-e16E, aos^UAS.cHa

(Marenda et al., 2006)

Scer\GAL4^en-e16E/msk^UAS.cLa is an enhancer of wing vein L5 phenotype of Scer\GAL4^en-e16E, aos^UAS.cHa

(Marenda et al., 2006)

Scer\GAL4^en-e16E/msk^UAS.cLa is an enhancer of wing vein phenotype of Scer\GAL4^en-e16E, aos^UAS.cHa

(Marenda et al., 2006)

msk^UAS.cLa, Scer\GAL4^en-e16E is an enhancer of wing vein phenotype of BacA\p35^UAS.cHa, Scer\GAL4^en-e16E

(Marenda et al., 2006)

Suppressor of

Statement

Reference

Scer\GAL4^en-e16E/msk^UAS.cLa is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^en-e16E, rho^hs.PSt

(Marenda et al., 2006)

Other

Statement

Reference

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, msk^UAS.cLa has wing phenotype

(Marenda et al., 2006)

Scer\GAL4^en-e16E, msk^UAS.cLa, rho^hs.PSt has wing phenotype

(Marenda et al., 2006)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, msk^UAS.cLa has wing disc posterior compartment | larval stage phenotype

(Marenda et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

mbc^D11.2/+ largely suppresses the muscle detachment phenotype of Scer\GAL4^twi.PU msk^Scer\UAS.cLa embryos.

(Liu et al., 2013)

The extra veins in the adult wing induced by rho^hs.PSt are strongly suppressed by the addition of ectopic msk^Scer\UAS.cLa (under the control of Scer\GAL4^en-e16E). Reduction of EGFR pathway function in rl^10a heterozygotes strongly suppresses the msk^Scer\UAS.cLa Scer\GAL4^en-e16E `Twiggy wing' (exhibiting a nearly normal anterior compartment and severely reduced posterior compartments) phenotype. Coexpression of argos^Scer\UAS.cHa and msk^Scer\UAS.cLa, under the control of Scer\GAL4^en-e16E, enhances the vein loss phenotype of argos to 100% in L4 and 100% in L5.

(Marenda et al., 2006)

Xenogenetic Interactions

Statement

Reference

Co-expression of BacA\p35^Scer\UAS.cHa with msk^Scer\UAS.cLa, under the control of Scer\GAL4^en-e16E, significantly enhances the adult vein loss observed in BacA\p35^Scer\UAS.cHa mutants, resulting in severely distorted wings with enlarged posterior compartments compared with the control anterior compartments (the `J.Lo wing').

(Marenda et al., 2006)

Complementation and Rescue Data

Partially rescues

Scer\GAL4^ey.PH/msk^UAS.cLa partially rescues msk⁵

(Pepple et al., 2007)

Comments

Expression of msk^Scer\UAS.cLa under the control of Scer\GAL4^ey.PH rescues eye development in mosaic animals with homozygous msk⁵ eyes (generated using the EGUF system), with the exception of minor disorganisation at the posterior margin.

(Pepple et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

23944

w^*; P{UAS-msk.L}47M1/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

msk^Scer\UAS.cLa

msk^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Lorenzen

(Lorenzen et al., 2001)

Secondary FlyBase IDs

References (7)

Report Sections

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