FlyBase Allele Report: Dmel\bcd[+t8.7]

General Information

Symbol

Dmel\bcd^+t8.7

Species

D. melanogaster

Name

FlyBase ID

FBal0042877

Feature type

allele

Associated gene

Dmel\bcd

Associated Insertion(s)

Carried in Construct

P{bcd}

Also Known As

Bcd^wt

Key Links

Genomic Maps

JBrowse

Transgenic product class

genomic_DNA

(Berleth et al., 1988)

wild_type

(Berleth et al., 1988)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

genomic_DNA

(Berleth et al., 1988)

wild_type

(Berleth et al., 1988)

Mutagen

in vitro construct

(Hoch et al., 1992, Berleth et al., 1988)

Progenitor genotype

Carried in construct

P{bcd}

Cytology

Description

8.7kb EcoRI fragment extending from walk position -42 to -33kb encoding the complete bcd functional unit.

(Berleth et al., 1988)

Allele components

Component

Use(s)

Regulatory region(s)

bcd

bcd_8.7kb

Encoded product / tool

bcd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

rescue_region

3R:6,754,864..6,763,604 [-]

Linked to:

EcoRI-EcoRI restriction fragment

Comment:

Position of restriction fragment on reference sequence inferred by FlyBase curator

(Berleth et al., 1988)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

No Assay Recorded

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 4

organism | 10-50% egg length

(Driever and Nusslein-Volhard, 1988)

Additional Information

Statement

Reference

Expression of bcdprotein from a single bcd^+t8.7 copy recapitulates the wild type bcdprotein expression pattern, demonstrating high bcd protein concentration atthe anterior tip of the embryo. Additional copies of bcd^+t8.7 increase thelevel of bcd protein in the embryo, where high protein concentrations aredetected at up to 50% egg length with four copies.

(Driever and Nusslein-Volhard, 1988)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

wild-type

(Bellaiche et al., 1996, Berleth et al., 1988)

Phenotype Manifest In

embryo | embryonic stage 4

(Blankenship and Wieschaus, 2001)

larval head, with bcd⁶

(Lebrecht et al., 2005)

Detailed Description

Statement

Reference

One copy of bcd^+t8.7 is sufficient to rescue bcd⁶/bcd⁶ lethality (100%) to levels similar to that of bcd⁶/+ embryos (8% compared to 4% unhatched in bcd⁶ heterozygotes). Of those that did hatch, 3% exhibit larval head defects.

(Lebrecht et al., 2005)

Embryos which are carrying bcd^+t8.7 and have six copies of bcd⁺ show a posterior shift of the pre-cephalic furrow domain (which has small yolk stalk diameters and a greater depth) during cellularisation. The anterior cellularisation domain of large yolk stalk diameters expands to cover close to half of the embryo. The anterior domain of lower nuclear density seen in wild-type embryos is expanded posteriorly.

(Blankenship and Wieschaus, 2001)

Embryos carrying seven copies of bcd⁺ (five in constructs and two wild type genomic copies) show failure to regulate cell number. Embryos have an enlarged head and the parasegments of the trunk are crowded into a reduced space, 5% reduction in number of bristles in A3, 31% reduction in cell number in parasegment 8 and histoblasts of the abdomen show a 40% reduction. One quarter of embryos hatch to larvae, these show a severe disruption of denticle pattern. Adults have gross defects in abdominal segmentation.

(Busturia and Lawrence, 1994)

P{bcd} can rescue bcd⁶ embryos to viable progeny.

(Hanes et al., 1994)

The head fold of females with 2 copies of bcd⁺ provided by P{bcd} is in the normal position. Females with 4 copies (two endogenous copies) have a very large head region and develop into normal adults.

(Berleth et al., 1988)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

bcd^+t8.7/bcd^+t8 is a suppressor of embryo | anterior phenotype of tor^RX

(Schaeffer et al., 2000)

bcd^+t8.7/bcd^+t8 is a suppressor of embryo | anterior phenotype of trk^unspecified

(Schaeffer et al., 2000)

bcd^+t8.7 is a suppressor of embryo | anterior phenotype of tsl⁴

(Schaeffer et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

bcd^+t8.7 rescues bcd⁶

(Lebrecht et al., 2005)

bcd^+t8.7 rescues bcd⁶

(Bellaiche et al., 1996)

bcd^+t8.7 rescues bcd⁶

(Berleth et al., 1988)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Bcd^wt

(Niessing et al., 2000)

bcd^+t8.7

Name Synonyms

Secondary FlyBase IDs

FBal0034226

References (20)

Report Sections

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