FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Klp61F06836
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General Information
Symbol
Dmel\Klp61F06836
Species
D. melanogaster
Name
FlyBase ID
FBal0043696
Feature type
allele
Associated gene
Dmel\Klp61F
Associated Insertion(s)
P{PZ}Klp61F06836
Carried in Construct
Also Known As
Klp61F2
Key Links
Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Wilson et al., 1997)
Mutagen
P-element activity
(Wilson et al., 1997, Heck et al., 1993)
Progenitor genotype
Associated Insertion(s)
P{PZ}Klp61F06836
Cytology
Description

Insertion of a P{PZ} element upstream and within 1.5kb of the P{PZ} element insertion in Klp61F07012.

(Heck et al., 1993)
Allele components
Component
Use(s)
Inserted element
P{PZ}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Klp61F07012/Klp61F06836 trans-heterozygotes display larval lethality.

      Klp61FUbi-p63E.T:Avic\GFP rescues the lethality of trans-heterozygous Klp61F07012/Klp61F06836 mutants. The rescued flies are fertile and can be propagated as stable transgenic lines. The rescued embryos display a decrease in the rate of prometaphase-to-metaphase spindle elongation compared with wild type embryos, which leads to a small decrease in subsequent spindle length.

      (Cheerambathur et al., 2008)

      The brains of homozygous larvae have a reduced frequency of anaphase figures, and an increased frequency of polyploid figures and overcondensed chromosomes compared to wild-type. The brains and imaginal discs are approximately half normal size.

      (Wilson et al., 1997)

      Homozygotes and hemizygotes die as late third instar larvae or early pupae. Neuroblasts isolated from third instar homozygous larvae show an increased mitotic index and complete lack of anaphase figures, indicating metaphase arrest. Chromosomes are hypercondensed compared to wild-type and highly polyploid cells are common.

      (Heck et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to complement

      Klp61F07012

      (BDGP Project Members, 1994-1999)

      Klp61F06345

      (BDGP Project Members, 1994-1999)
      Rescued by

      Klp61F06836/Klp61F07012 is rescued by Klp61FUbi-p63E.GFP

      (Cheerambathur et al., 2008)
      Comments

      Klp61FUbi-p63E.T:Avic\GFP rescues the lethality of trans-heterozygous Klp61F07012/Klp61F06836 mutants. The rescued flies are fertile and can be propagated as stable transgenic lines.

      (Cheerambathur et al., 2008)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer

      A. Spradling.

      Comments
      Comments

      Excision of the P{PZ} element is accompanied by reversion of lethality and the abnormal mitotic phenotype.

      (Heck et al., 1993)

      Klp61F alleles form the following phenotypic series, from the most to the least severe: Klp61Furc-1 > Klp61Furc-3 > Klp61F06836, Klp61F07012 > Klp61F06345 > Klp61Furc-4.

      (Wilson et al., 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Klp61F06836
      Klp61F2
      (Wilson et al., 1997, Heck et al., 1993)
      klp61f6836
      (Cheerambathur et al., 2008)
      l(3)0701206836
      Name Synonyms
      Secondary FlyBase IDs
        References (6)