FlyBase Allele Report: Dmel\Klp61F[06345]

General Information

Symbol

Dmel\Klp61F⁰⁶³⁴⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0043695

Feature type

allele

Associated gene

Dmel\Klp61F

Associated Insertion(s)

P{PZ}Klp61F⁰⁶³⁴⁵

Carried in Construct

Also Known As

klp61F¹

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Wilson et al., 1997)

Mutagen

P-element activity

(Wilson et al., 1997, Heck et al., 1993)

Progenitor genotype

Associated Insertion(s)

P{PZ}Klp61F⁰⁶³⁴⁵

Cytology

Description

Insertion of a P{PZ} element upstream and within 1.5kb of the P{PZ} element insertion in Klp61F⁰⁷⁰¹².

(Heck et al., 1993)

Allele components

Component

Use(s)

Inserted element

P{PZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | recessive

(Heck et al., 1993)

lethal | recessive

(Spradling et al., 1999, Harvie et al., 1998, Wilson et al., 1997, Heck et al., 1993)

Phenotype Manifest In

aster & spermatocyte (with Klp61F⁰⁷⁰¹²)

(Wilson et al., 2004)

centriole & spermatocyte

(Wilson et al., 1997)

centrosome & spermatocyte (with Klp61F⁰⁷⁰¹²)

(Wilson et al., 2004)

imaginal disc | larval stage

(Wilson et al., 1997)

larval brain

(Wilson et al., 1997)

nuclear chromosome

(Heck et al., 1993)

spermatocyte fusome

(Wilson, 1999)

spindle & spermatocyte (with Klp61F⁰⁷⁰¹²)

(Wilson et al., 2004)

Detailed Description

Statement

Reference

Acentrosomal meiotic spermatocytes of Klp61F⁰⁷⁰¹²/Klp61F⁰⁶³⁴⁵ transheterozygotes exhibit microtubules, but do not show microtubule asters or detectable spindle structures.

(Wilson et al., 2004)

The fusome in Klp61F⁰⁶³⁴⁵ testes appears to be fragmented.

(Wilson, 1999)

The brains of homozygous and hemizygous larvae have a reduced frequency of anaphase figures, and an increased frequency of polyploid figures and overcondensed chromosomes compared to wild-type. The brains of Klp61F⁰⁶³⁴⁵/Klp61F^urc-4 larvae have a less severe mitotic phenotype. 28.4% of the mitotic spindles in homozygous larval brain cells are monopolar, 65.2% are bipolar but monastral (only one half-spindle appears to be organised by a centrosome with a focus of γ tubulin) and 6.4% are normal biastral bipolar spindles. 13.3% of the mitotic spindles in Klp61F⁰⁶³⁴⁵/Klp61F^urc-4 larval brain cells are monopolar, 25.8% are bipolar but monastral (only one half-spindle appears to be organised by a centrosome with a focus of γ tubulin) and 60.9% are normal biastral bipolar spindles. The brains and imaginal discs are small compared to wild-type in approximately 50% of homozygous and hemizygous larvae, and often similar to wild-type in size and morphology in Klp61F⁰⁶³⁴⁵/Klp61F^urc-4 larvae. Nearly all homozygous spermatocytes are equal in size and contain equivalent amounts of chromatin. The spermatocytes contain a variable number of centriole pairs, the most common being 0 (35% of spermatocytes), 2 (25% of spermatocytes) and 4 (24% of spermatocytes) centriole pairs, and 10% of spermatocytes contain an odd number of centriole pairs.

(Wilson et al., 1997)

Homozygotes and hemizygotes die as late third instar larvae or early pupae. Neuroblasts isolated from third instar homozygous larvae show an increased mitotic index and complete lack of anaphase figures, indicating metaphase arrest. Chromosomes are hypercondensed compared to wild-type and highly polyploid cells are common.

(Heck et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Klp61F[+]/Klp61F⁰⁶³⁴⁵ is an enhancer of visible phenotype of Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

Other

Statement

Reference

Klp61F^urc-1/Klp61F⁰⁶³⁴⁵, ncd¹/ncd⁹ has partially lethal phenotype

(Wilson et al., 2004)

Klp61F⁰⁷⁰¹²/Klp61F⁰⁶³⁴⁵, ncd¹/ncd⁹ has viable phenotype

(Wilson et al., 2004)

Klp61F⁰⁶³⁴⁵, ncd⁹ has partially lethal phenotype

(Wilson et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Klp61F⁰⁶³⁴⁵ has spindle & neuroblast & larva phenotype, suppressible by ncd⁹

(Wilson et al., 2004)

Enhancer of

Statement

Reference

Klp61F[+]/Klp61F⁰⁶³⁴⁵ is an enhancer of eye phenotype of Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

Other

Statement

Reference

Klp61F⁰⁶³⁴⁵, ncd¹ has chromatin & spermatocyte phenotype

(Wilson et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Klp61F⁰⁶³⁴⁵ ncd⁹/Klp61F^urc-1 ncd¹ adults are recovered at 28% of the frequency expected for full viability. Klp61F⁰⁶³⁴⁵ ncd⁹ double mutant adults are recovered at 46% of the frequency expected for full viability. Biastral spindles in Klp61F⁰⁶³⁴⁵ ncd¹ spermatocytes sometimes show multiple masses of γ-tubulin at spindle poles and an apparently polyploid complement of chromatin. More than 96% of spindles in Klp61F⁰⁶³⁴⁵ ncd⁹ mutant brains are biastral.

(Wilson et al., 2004)

Xenogenetic Interactions

Statement

Reference

One copy of Klp61F⁰⁶³⁴⁵ enhances the rough eye phenotype seen in flies expressing Hsap\MAPT^GMR.Ex.PJ.

(Ambegaokar and Jackson, 2011)

Complementation and Rescue Data

Fails to complement

Klp61F⁰⁷⁰¹²

(BDGP Project Members, 1994-1999)

Klp61F⁰⁶⁸³⁶

(BDGP Project Members, 1994-1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

32012

P{PZ}Klp61F⁰⁶³⁴⁵/TM6B, Tb¹

Notes on Origin

Discoverer

A. Spradling.

Comments

Excision of the P{PZ} element is accompanied by reversion of lethality and the abnormal mitotic phenotype.

(Heck et al., 1993)

Complements: l(3)02640⁰²⁶⁴⁰.

(BDGP Project Members, 1994-1999)

Klp61F alleles form the following phenotypic series, from the most to the least severe: Klp61F^urc-1 > Klp61F^urc-3 > Klp61F⁰⁶⁸³⁶, Klp61F⁰⁷⁰¹² > Klp61F⁰⁶³⁴⁵ > Klp61F^urc-4.

(Wilson et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

KLP61F¹

(Wilson, 1999)

Klp61⁰⁶³⁴⁵

(Ambegaokar and Jackson, 2011)

Klp61F⁰⁶³⁴⁵

Klp61F¹

(Wilson et al., 1997, Heck et al., 1993)

klp61F⁰⁶³⁴⁵

(Hwang et al., 2020)

klp61F¹

(Wilson et al., 2004)

l(3)06345

(Spradling et al., 1999)

l(3)07012⁰⁶³⁴⁵

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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