FlyBase Allele Report: Dmel\Klp61F[urc-1]

General Information

Symbol

Dmel\Klp61F^urc-1

Species

D. melanogaster

Name

FlyBase ID

FBal0032985

Feature type

allele

Associated gene

Dmel\Klp61F

Associated Insertion(s)

P{lacW}Klp61F^urc-1

Carried in Construct

Also Known As

klp61F⁴

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Wilson et al., 1997)

Mutagen

P-element activity

(Wilson et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}Klp61F^urc-1

Cytology

Description

Insertion of four nucleotides near the start codon that shifts the translational reading frame. This is predicted to produce a truncated protein of 66 amino acids that includes the amino terminal 40 amino acids of Klp61F.

(Wilson et al., 2004)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease

modeled by Hsap\MAPT^{UAS.0N4R.Tag:FLAG}

(Bougé and Parmentier, 2016)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Wilson and Fuller, 1992)

abnormal mitotic cell cycle | recessive

(Wilson et al., 1997)

lethal | recessive

(Wilson et al., 1997)

Phenotype Manifest In

imaginal disc | larval stage

(Wilson et al., 1997)

larval brain

(Wilson et al., 1997)

nuclear lamina & neuroblast & larva

(Wilson et al., 2004)

spermatid (with Klp61F⁰⁷⁰¹²)

(Wilson et al., 2004)

spermatocyte (with Klp61F⁰⁷⁰¹²)

(Wilson et al., 2004)

spindle & spermatocyte (with Klp61F⁰⁷⁰¹²)

(Wilson et al., 2004)

testis

(Wilson et al., 2004)

Detailed Description

Statement

Reference

The nuclear lamina in monopolar Klp61F^urc-1 cells show involutions that extend towards centrally located centrosomes. Testes are not generated in Klp61F^urc-1 homozygous mutants. A number of transheterozygous Klp61F⁰⁷⁰¹²/Klp61F^urc-1 spermatocytes exhibit several small nuclei at the poles of telophase spindles and some post-meiotic spermatids also contain micronuclei. Fusiform structures, indicative of a bipolar spindle in wild-type spermatocytes are not detected in Klp61F⁰⁷⁰¹²/Klp61F^urc-1 transheterozygote spermatocytes.

(Wilson et al., 2004)

The brains of homozygous and hemizygous larvae have no anaphase figures, and an increased frequency of polyploid figures and overcondensed chromosomes compared to wild-type. 87.8% of the mitotic spindles in homozygous larval brain cells are monopolar, with the remainder being bipolar but monastral (only one half-spindle appears to be organised by a centrosome with a focus of γ tubulin). No normal biastral bipolar spindles are seen. The brains are very small compared to wild-type and there are no recognisable imaginal discs.

(Wilson et al., 1997)

Primary defect is an inability of centrosomes to separate and organise a bipolar spindle. Mitotic figures are highly polyploid consisting of overcondensed chromosomes in apparent monopolar configurations.

(Wilson and Fuller, 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Klp61F^urc-1 has lethal | larval stage phenotype, suppressible by ncd¹

(Wilson et al., 2004)

Enhancer of

Statement

Reference

Klp61F^urc-1 is an enhancer of visible | adult stage phenotype of Hsap\MAPT^{UAS.0N4R.Tag:FLAG}, Scer\GAL4^ptc.PU

(Bougé and Parmentier, 2016)

Klp61F^urc-1 is an enhancer of visible | adult stage phenotype of Hsap\MAPT^{UAS.0N4R.Tag:FLAG}, Scer\GAL4^GMR.PU

(Bougé and Parmentier, 2016)

Klp61F^urc-1 is an enhancer of visible | adult stage phenotype of Scer\GAL4^ptc.PU, tau^UAS.cMa

(Bougé and Parmentier, 2016)

Other

Statement

Reference

Klp61F^urc-1, ncd¹ has increased occurrence of cell division phenotype

(Wilson et al., 2004)

Klp61F^urc-1/Df(3L)bab-PG, ncd¹ has lethal phenotype

(Wilson et al., 2004)

Klp61F^urc-1/Klp61F⁰⁶³⁴⁵, ncd¹/ncd⁹ has partially lethal phenotype

(Wilson et al., 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

Klp61F^urc-1 has aster & neuroblast & larva phenotype, suppressible by ncd¹

(Wilson et al., 2004)

Klp61F^urc-1 has spindle & neuroblast & larva phenotype, suppressible by ncd¹

(Wilson et al., 2004)

Enhancer of

Statement

Reference

Klp61F^urc-1 is an enhancer of wing blade phenotype of Hsap\MAPT^{UAS.0N4R.Tag:FLAG}, Scer\GAL4^ptc.PU

(Bougé and Parmentier, 2016)

Klp61F^urc-1 is an enhancer of eye phenotype of Hsap\MAPT^{UAS.0N4R.Tag:FLAG}, Scer\GAL4^GMR.PU

(Bougé and Parmentier, 2016)

Klp61F^urc-1 is an enhancer of interommatidial bristle phenotype of Hsap\MAPT^{UAS.0N4R.Tag:FLAG}, Scer\GAL4^GMR.PU

(Bougé and Parmentier, 2016)

Klp61F^urc-1 is an enhancer of wing blade phenotype of Scer\GAL4^ptc.PU, tau^UAS.cMa

(Bougé and Parmentier, 2016)

Other

Statement

Reference

Hsap\MAPT^{UAS.0N4R.Tag:FLAG}, Klp61F^urc-1, Scer\GAL4^GMR.PU has ommatidium | adult stage phenotype

(Bougé and Parmentier, 2016)

Klp61F^urc-1, ncd¹ has nuclear lamina & neuroblast & larva phenotype

(Wilson et al., 2004)

Klp61F^urc-1/Klp61F⁰⁷⁰¹², ncd¹ has nuclear lamina & neuroblast & larva phenotype

(Wilson et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The reduced size of the L3-L4 intervein region (measured at the wing margin) observed in flies expressing tau^Scer\UAS.cMa under the control of Scer\GAL4^ptc.PU is decreased further by combination with Klp61F^urc-1 in heterozygous state.

(Bougé and Parmentier, 2016)

Klp61F⁰⁶³⁴⁵ ncd⁹/Klp61F^urc-1 ncd¹ adults are recovered at 28% of the frequency expected for full viability. Klp61F^urc-1 ncd¹ double mutants survive longer as third instar larvae and show increased cell proliferation in comparison to Klp61F^urc-1 mutants. Klp61F^urc-1 ncd¹ cells have a monopolar organisation of chromosomes and centrosomes, showing involutions that extend towards centrally located centrosomes. Klp61F⁰⁷⁰¹² ncd¹/Klp61F^urc-1 ncd¹ cells have a monopolar organisation of chromosomes and centrosomes, showing nuclear lamina involutions that extend towards centrally located centrosomes. Biastral spindles are not found in Klp61F^urc-1 mutants, but comprise more than 66% of spindles in Klp61F^urc-1 ncd¹ mutants.

(Wilson et al., 2004)

Xenogenetic Interactions

Statement

Reference

The reduced size of the L3-L4 intervein region (measured at the wing margin) characteristic for adult flies expressing Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG} under the control of Scer\GAL4^ptc.PU is decreased further by combination with Klp61F^urc-1 in heterozygous state.

The rough eye phenotype (missing interommatidial bristles) observed in adult flies expressing single copy of Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PU is exacerbated further (more missing bristles, ommatidial fusion) by combination with Klp61F^urc-1 in heterozygous state. The resulting phenotype is similar or even stronger than that seen in flies expressing two copies of Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG}.

(Bougé and Parmentier, 2016)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

35508

Klp61F^urc-1/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Klp61F alleles form the following phenotypic series, from the most to the least severe: Klp61F^urc-1 > Klp61F^urc-3 > Klp61F⁰⁶⁸³⁶, Klp61F⁰⁷⁰¹² > Klp61F⁰⁶³⁴⁵ > Klp61F^urc-4.

(Wilson et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

KLP61F⁴

(Wilson et al., 1997)

Klp61F^URC-1

(Hasan et al., 2024)

Klp61F^urc-1

(Bougé and Parmentier, 2016)

klp61F⁴

(Wilson et al., 2004)

klp61f⁴

(Cheerambathur et al., 2008)

urc¹

(Wilson et al., 1997)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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