Ectopic expression of Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG} under the control of Scer\GAL4^ptc.PU results in an increased number of cells in mitosis in the third instar larval wing disc, however there is no corresponding increase in S-phase cell numbers, suggesting that the cell-cycle duration is not shorter compared to controls. Levels of cell death (detected by Caspase-3 staining) are also increased in the Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG} expressing region of the wing disc and the size of the corresponding area of the adult wing - the intervein region between L3 and L4 veins - is significantly reduced (measured at the wing margin) compared to controls.

Expression of Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG} under the control of any of the following Scer\GAL4^ptc.PU, Scer\GAL4^dpp.PU or Scer\GAL4^Bx-MS1096 leads to mitotic spindle defects - monopolar spindle - and chromosomal poly- and aneuploidy in third instar larval wing disc cells. Cells expressing Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG} (under the Scer\GAL4^ptc.PU driver) show failure of the duplicated centrosomes to move apart to the opposite poles, thus leading to the monopolar mitotic spindle.

Expression of Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PU leads to rough eye phenotype in the adults, expression of single copy of the transgene has a rather mild effect: missing bristles but almost no ommatidial disorganization, expression of two copies of Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG} results in stronger phenotype with more bristles missing and ommatidial disorganization at the posterior side of the eye.

Expression of Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG} under the control of Scer\GAL4^insc-Mz1407 results in mitotic defects (monopolar spindle, chromosomal aneuploidy) in the brain neuroblasts of third instar larvae.

Co-expression of Hsap\MAPT^{Cter.Scer\UAS.T:Zzzz\FLAG} together with Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG} under the control of Scer\GAL4^GMR.PU results in rough eye phenotype (missing interommatidial bristles, ommatidial fusions) in the adults. The phenotype is the same as when two copies of full-length Hsap\MAPT^{Scer\UAS.0N4R.T:Zzzz\FLAG} are used.

Hsap\MAPT^{UAS.0N4R.Tag:FLAG}, Scer\GAL4^GMR.PU has visible | adult stage phenotype, suppressible by Klp61F^Ubi-p63E.GFP

Hsap\MAPT^{UAS.0N4R.Tag:FLAG}, Klp61F^urc-1, Scer\GAL4^GMR.PU has ommatidium | adult stage phenotype