FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ndl9
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General Information
Symbol
Dmel\ndl9
Species
D. melanogaster
Name
FlyBase ID
FBal0046158
Feature type
allele
Associated gene
Dmel\ndl
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

hypomorphic allele - molecular evidence

Mutagen
    Nature of the Allele
    Allele class

    hypomorphic allele - genetic evidence

    (LeMosy and Hashimoto, 2000)

    hypomorphic allele - molecular evidence

    (LeMosy et al., 2000)
    Mutagen
    Progenitor genotype
    Cytology
    Description

    Nucleotide substitution: T3608A. Amino acid replacement: C1114S. Mutation is outside, but close to, the N terminus of the catalytic domain.

    (LeMosy et al., 2000)
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    point_mutation
    3L:6,599,159..6,599,159 [-]
    Nucleotide change:

    T6599159A

    Reported nucleotide change:

    T3608A

    Amino acid change:

    C1114S | ndl-PA

    Reported amino acid change:

    C1114S

    Comment:

    Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

    (LeMosy et al., 2000)
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        In eggs from mutant mothers, the vitelline membrane is permeable to Neutral red dye. There appears to be a correlation between the severity of the dorsalisation phenotype and the penetrance of vitelline membrane permeability phenotype, as both are increased at the non-permissive temperature (22oC).

        (LeMosy and Hashimoto, 2000)

        Allele has partial activity in dorsoventral polarity establishment.

        (LeMosy et al., 2000)

        Class II allele. Affected embryos show temperature sensitive dorsalization. The degree of dorsalization varies from so mild as to allow hatching (approximately 25% at 18oC) to completely dorsalized (approximately 25% at 18oC). Eggs are somewhat fragile.

        (Hong and Hashimoto, 1996)

        The percentage of eggs laid by ndl9/ndl1 females that hatch following temperature shifts demonstrate the temperature sensitive period for ndl begins around stage 7 of oogenesis and ends roughly 2 hours after fertilisation.

        (Hong and Hashimoto, 1995)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference
        Xenogenetic Interactions
        Statement
        Reference
        Complementation and Rescue Data
        Complements

        ndlLP-2

        (LeMosy et al., 2000)
        Partially complements

        ndl1

        (LeMosy and Hashimoto, 2000)
        Fails to complement

        ndl3

        (LeMosy and Hashimoto, 2000)
        Rescued by

        ndl9 is rescued by ndlGAG.S-A

        (Turcotte and Hashimoto, 2002)
        Comments

        ndl9/ndl1 embryos exhibit a weaker dorsalisation phenotype and vitelline membrane permeability phenotype than either ndl9 or ndl1 over a deficiency. ndl9/ndl3 are as severely defective in both dorsal ventral patterning and eggshell integrity as ndl3 itself.

        (LeMosy and Hashimoto, 2000)

        80% of embryos derived from ndl1/ndlLP-2 or ndl9/ndlLP-2 females hatch at 22oC. ndlLP-1, ndl2 and ndlrm5 show dominant negative inhibition of ndl9.

        (LeMosy et al., 2000)
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        Comments
        Comments

        Class II allele.

        (LeMosy et al., 2000)

        Complements the Class I ndl15 allele, and the Class II ndl1 allele.

        (Hong and Hashimoto, 1996)
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (1)
        Reported As
        Symbol Synonym
        ndl9
        Name Synonyms
        Secondary FlyBase IDs
          References (7)