FlyBase Gene Report: Dmel\ndl

General Information

Symbol

Dmel\ndl

Species

D. melanogaster

Name

nudel

Annotation Symbol

CG10129

Feature Type

protein_coding_gene

FlyBase ID

FBgn0002926

Gene Model Status

Current

Stock Availability

8 publicly available

Enzyme Name (EC)

Gene Summary

nudel (ndl) encodes a multi-functional protein with serine protease activity. It is required for both eggshell biogenesis and embryonic dorsoventral patterning. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Gene Group
UniProtKB
Red Book
Interactive Fly

Also Known As

SP89

Key Links

Genomic Location

Cytogenetic map

65B5-65B5

Sequence location

3L:6,593,938..6,603,366 [-]

Recombination map

3-17

RefSeq locus

NT_037436 REGION:6593938..6603366

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (11 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables serine-type endopeptidase activity

inferred from mutant phenotype

(Mineo et al., 2017, LeMosy et al., 1998)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables serine-type endopeptidase activity

inferred from sequence model

(Cao and Jiang, 2018, Ross et al., 2003)

inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR018114

(InterPro Project Members, 2004-)

Biological Process (8 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

involved_in dorsal/ventral axis specification

inferred from mutant phenotype

(Mineo et al., 2017, LeMosy et al., 1998, Hong and Hashimoto, 1996, Hong and Hashimoto, 1995, Stein et al., 1991)

inferred from genetic interaction with FLYBASE:ea; FB:FBgn0000533

(Chasan et al., 1992)

involved_in egg activation

inferred from mutant phenotype

(Tadros et al., 2003)

involved_in egg chorion assembly

inferred from mutant phenotype

(Hong and Hashimoto, 1996)

involved_in protein autoprocessing

inferred from mutant phenotype

(LeMosy et al., 1998)

involved_in protein processing

inferred from mutant phenotype

(Mineo et al., 2017)

involved_in Toll receptor ligand protein activation cascade

inferred from mutant phenotype

(LeMosy et al., 1998, Misra et al., 1998)

inferred from direct assay

(LeMosy et al., 2001)

involved_in zymogen activation

inferred from mutant phenotype

(Steen et al., 2010, LeMosy et al., 2001, LeMosy et al., 1998)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in proteolysis

inferred from electronic annotation with InterPro:IPR001254, InterPro:IPR018114

(InterPro Project Members, 2004-)

inferred from sequence model

(Ross et al., 2003)

Cellular Component (2 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Mineo et al., 2017)

located_in perivitelline space

inferred from direct assay

(LeMosy et al., 1998)

Terms Based on Predictions or Assertions (0 terms)

Gene Group (FlyBase)

DORSAL GROUP

S1A SERINE PROTEASES - TRYPSIN-LIKE

Pathway (FlyBase)

EXTRACELLULAR SPATZLE ACTIVATING PATHWAY CORE COMPONENTS

Protein Family (UniProt)

Belongs to the peptidase S1 family. (P98159)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

serine-type endopeptidase activity

Summaries

Gene Snapshot

nudel (ndl) encodes a multi-functional protein with serine protease activity. It is required for both eggshell biogenesis and embryonic dorsoventral patterning. [Date last reviewed: 2019-03-14]

Gene Group (FlyBase)

DORSAL GROUP -: The Dorsal group genes encode components of the Toll pathway and were initially identified by maternal effect mutations resulting in the dorsalization of the embryo. (Adapted from FBrf0091014 and FBrf0223077).
S1A SERINE PROTEASES - TRYPSIN-LIKE -: Trypsin-like serine proteases of the S1A family are endopeptidases that cleave amide substrates following Arginine or Lysine at the P1 position. (Adapted from FBrf0239469).

Pathway (FlyBase)

EXTRACELLULAR SPATZLE ACTIVATING PATHWAY CORE COMPONENTS -: In Drosophila, activation of the Toll pathway is controlled by the generation of a cleaved, active, Toll-binding form of spatzle ligand. Proteolytic activation of spatzle ligand lies downstream of several zymogen activation cascades that are initiated by different cues. In dorsal-ventral patterning, localized activation of spz results in ventral nuclear accumulation of dl. During viral and fungal and gram positive bacterial immune challenge, zymogen cascades are activated by extracellular pattern recognition receptors or virulence factor-mediated cleavage of the zymogen persephone (psh). (Adapted from FBrf0091014 and FBrf0223077).

Protein Function (UniProtKB)

Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo (PubMed:7671306). A protease cascade involving ndl, gd, snk and ea results in activation of the spz Toll receptor ligand; acts upstream of gd, snk and ea and is required for proteolytic processing of gd (PubMed:20605458). Activation of ea requires activation of the ndl-gd-snk protease cascade and sulfation of a vitelline membrane component by pip (PubMed:20605458). Localized activation of the Toll receptor in the ventral region of the embryo defines cell identities along the dorsal-ventral continuum (Probable).

(UniProt, P98159)

Phenotypic Description (Red Book; Lindsley and Zimm 1992)

ndl: nudel

Maternal effect mutation producing totally dorsalized embryos. Spacing of transverse stripes of ftz protein altered in mutant embryos (Carroll, Winslow, Twombly, and Scott, 1987, Development 99: 327-32).

Summary (Interactive Fly)

serine protease - docking protein - dorsal group - part of a signaling cascade that determines dorsal/ventral polarity of the embryo - required for eggshell biogenesis

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ndl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry P98159)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Gene model reviewed during 5.46

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ndl-RA

FBtr0076984

NM_079223

8230

2616

Additional Transcript Data and Comments

Reported size (kB)

8.4 (northern blot)

(Hong and Hashimoto, 1995)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ndl-PA

FBpp0076693

292.5

2616

5.77

P98159

NP_523947

AAF50656

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

2616 (aa); 292 (kD predicted)

(Hong and Hashimoto, 1995)

Comments

External Data

Post Translational Modification

Requires cleavage for activation (presumably).

(UniProt, P98159)

Crossreferences

InterPro - A database of protein families, domains and functional sites

MEROPS - An information resource for peptidases (also termed proteases, proteinases and proteolytic enzymes) and the proteins that inhibit them.

S01.950

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

P98159

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ndl using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ndl_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.34

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

oogenesis stage S7 -- S10

follicle cell

(Hong and Hashimoto, 1995)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage | female

ovary

(Hong and Hashimoto, 1995)

Additional Descriptive Data

ndl transcripts are expressed almost exclusively in follicle cells. They are first detected at stage S7 of oogenesis but reach a peak at stages S9 and S10. In the majority of egg chambers, the transcript is asymmetrically distributed with a higher level seen in ventral than in dorsal follicle cells. The transcript decreases in abundance dramatically in late stage S10 and is undetectable by stage S11.

(Hong and Hashimoto, 1995)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Proteolytically processed segments of ndl protein are secreted into the perivitelline space and stably localized.

(Stein et al., 2008)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in extracellular region

inferred from direct assay

(Mineo et al., 2017)

located_in perivitelline space

inferred from direct assay

(LeMosy et al., 1998)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ndl in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ndl

View exonic expression by tissue for Dmel\ndl

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38738/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002926

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002926

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002926

Flygut - An atlas of the Drosophila adult midgut

FBgn0002926

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 23 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 10 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ndl

Transgenic constructs containing regulatory region of ndl

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(3L)03844

(Stein et al., 2008, Hong and Hashimoto, 1996, Hong and Hashimoto, 1995)

Df(3L)CH12

(Turcotte and Hashimoto, 2002, Hong and Hashimoto, 1996, Hong and Hashimoto, 1995)

Gene not disrupted in

Df(3L)CH39

(Hong and Hashimoto, 1995)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | maternal effect | recessive

lethal - all die during embryonic stage | recessive

ndl¹

partially lethal | maternal effect | recessive

ndl^LP-2

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ndl^GD2337

viable, with Scer\GAL4^Mef2.PR

ndl^GD2337

viable, with Scer\GAL4^pnr-MD237

ndl^GD2337

Other Phenotypes

Allele

some die during embryonic stage | recessive

ndl¹

Phenotype manifest in

Allele

egg

egg | maternal effect

egg chorion | maternal effect

embryo

ndl⁹

embryo | blastoderm stage | maternal effect

embryo | ventral

ndl^unspecified

embryonic/first instar larval cuticle

ndl¹

embryonic epidermis | maternal effect

embryonic epidermis | ventral

ndl³

vitelline membrane

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (29)

Hsap\C1R

1 of 14

Yes

Hsap\CFI

1 of 14

Yes

Hsap\CORIN

1 of 14

Yes

Hsap\CSMD3

1 of 14

Yes

Hsap\CUBN

1 of 14

Yes

Hsap\HPN

1 of 14

Yes

Hsap\LRP1B

1 of 14

Yes

Hsap\LRP1

1 of 14

Yes

Hsap\LRP2

1 of 14

Yes

Hsap\LRP4

1 of 14

Yes

Hsap\MASP1

1 of 14

Yes

Hsap\MASP2

1 of 14

Yes

Hsap\PROC

1 of 14

Yes

Hsap\PRSS8

1 of 14

Yes

Hsap\PRSS12

1 of 14

Yes

Hsap\PRSS22

1 of 14

Yes

Hsap\PRSS27

1 of 14

Yes

Hsap\SORL1

1 of 14

Yes

Hsap\SSPOP

1 of 14

Yes

Hsap\ST14

1 of 14

Yes

Hsap\TMPRSS2

1 of 14

Yes

Hsap\TMPRSS3

1 of 14

Yes

Hsap\TMPRSS4

1 of 14

Yes

Hsap\TMPRSS5

1 of 14

Yes

Hsap\TMPRSS6

1 of 14

Yes

Hsap\TMPRSS7

1 of 14

Yes

Hsap\TMPRSS11B

1 of 14

Yes

Hsap\TMPRSS13

1 of 14

Yes

Hsap\TMPRSS15

1 of 14

Yes

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (29)

Rnor\C1r

1 of 14

Yes

Rnor\C1s

1 of 14

Yes

Rnor\Cfi

1 of 14

Yes

Rnor\Corin

1 of 14

Yes

Rnor\F12

1 of 14

Yes

Rnor\Hpn

1 of 14

Yes

Rnor\Lrp1

1 of 14

Yes

Rnor\Lrp2

1 of 14

Yes

Rnor\Lrp4

1 of 14

Yes

Rnor\Masp1

1 of 14

Yes

Rnor\Masp2

1 of 14

Yes

Rnor\Prss8

1 of 14

Yes

Rnor\Prss12

1 of 14

Yes

Rnor\Prss22

1 of 14

Yes

Rnor\Prss27

1 of 14

Yes

Rnor\Prss44

1 of 14

Yes

Rnor\Sorl1

1 of 14

Yes

Rnor\St14

1 of 14

Yes

Rnor\Tmprss2

1 of 14

Yes

Rnor\Tmprss3

1 of 14

Yes

Rnor\Tmprss4

1 of 14

Yes

Rnor\Tmprss5

1 of 14

Yes

Rnor\Tmprss6

1 of 14

Yes

Rnor\Tmprss7

1 of 14

Yes

Rnor\Tmprss9

1 of 14

Yes

Rnor\Tmprss11b

1 of 14

Yes

Rnor\Tmprss11g

1 of 14

Yes

Rnor\Tmprss13

1 of 14

Yes

Rnor\Tmprss15

1 of 14

Yes

Mus musculus (laboratory mouse) (30)

Mmus\C1ra

1 of 14

Yes

Mmus\C1rb

1 of 14

Yes

Mmus\C1s1

1 of 14

Yes

Mmus\C1s2

1 of 14

Yes

Mmus\Cfi

1 of 14

Yes

Mmus\Corin

1 of 14

Yes

Mmus\Csmd3

1 of 14

Yes

Mmus\Gm19410

1 of 14

Yes

Mmus\Hpn

1 of 14

Yes

Mmus\Lrp1b

1 of 14

Yes

Mmus\Lrp1

1 of 14

Yes

Mmus\Lrp4

1 of 14

Yes

Mmus\Masp1

1 of 14

Yes

Mmus\Masp2

1 of 14

Yes

Mmus\Prss8

1 of 14

Yes

Mmus\Prss12

1 of 14

Yes

Mmus\Prss22

1 of 14

Yes

Mmus\Prss27

1 of 14

Yes

Mmus\Sorl1

1 of 14

Yes

Mmus\Sspo

1 of 14

Yes

Mmus\St14

1 of 14

Yes

Mmus\Tmprss2

1 of 14

Yes

Mmus\Tmprss3

1 of 14

Yes

Mmus\Tmprss4

1 of 14

Yes

Mmus\Tmprss5

1 of 14

Yes

Mmus\Tmprss6

1 of 14

Yes

Mmus\Tmprss7

1 of 14

Yes

Mmus\Tmprss11g

1 of 14

Yes

Mmus\Tmprss13

1 of 14

Yes

Mmus\Tmprss15

1 of 14

Yes

Xenopus tropicalis (Western clawed frog) (37)

Xtro\LOC100491119

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\emx2

1 of 13

Xtro\gprin3

1 of 13

Yes

Xtro\krt8.1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100495333

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\prss8l.5 loc108703873

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\tmprss2.12

1 of 13

Xtro\tmprss2.14

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (39)

Drer\si:dkey-16l2.17

2 of 14

Yes

Drer\cfi

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\svh-1

2 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Cele\mua-3

1 of 14

Cele\try-1

1 of 14

Anopheles gambiae (African malaria mosquito) (17)

Agam\AgaP_AGAP007280

10 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Agam\AgaP_AGAP008649

1 of 12

Agam\AgaP_AGAP010820

1 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ndl. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (39)

CG1632

2 of 13

teq

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

mas

1 of 13

Ser7

1 of 13

Sp212

1 of 13

tpr

1 of 13

trol

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

ndl - cnn

anti bait coimmunoprecipitation, peptide massfingerprinting

(Habermann et al., 2012)

Summary of Genetic Interactions

Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

64189

DroID - A comprehensive database of gene and protein interactions.

FBgn0002926

MIST (protein-protein) - An integrated Molecular Interaction Database

38738

Pathways

Signaling Pathways (FlyBase)

EXTRACELLULAR SPATZLE ACTIVATING PATHWAY CORE COMPONENTS

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-17

Cytogenetic map

65B5-65B5

Sequence location

3L:6,593,938..6,603,366 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65B5-65B5

Limits computationally determined from genome sequence between P{PZ}l(3)02094⁰²⁰⁹⁴ and P{lacW}l(3)L4060^L4060

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

3-17

(FlyBase, 2016, Tearle and Nusslein-Volhard, 1987)

3-15.1

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

56238

y¹ w^*; Mi{MIC}ndl^MI10748

9036

mwh¹ ndl⁷ red¹ e¹/TM1

9038

mwh¹ ndl^rm5 red¹ e¹/TM1

60282

y¹ w^*; Mi{PT-GFSTF.1}ndl^{MI10748-GFSTF.1}

65142

y¹ sc^* v¹ sev²¹; P{TRiP.HMC03171}attP40

NIG-Fly

HMJ23742

y¹ v¹; P{TRiP.HMJ23742}attP40/CyO

VDRC

v102818

P{KK103890}VIE-260B

v44385

w¹¹¹⁸; P{GD2337}v44385

Genomic Clones (23)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (6)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0002926

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

The non-protease domains of ndl do not influence the signaling pathway that establishes embryonic DV polarity.

(Stein et al., 2008)

ndl has an integral role in the biogenesis of the vitelline membrane and may function in embryonic dorsal ventral patterning through creation of a matrix structure necessary for activity of the dorsal ventral protease cascade.

(LeMosy and Hashimoto, 2000)

Catalytic activity of the protease domain of the modular ndl protein is required for dorsoventral patterning of the embryo.

(LeMosy et al., 2000)

The serine protease activity of ndl protein is essential for embryonic dorsoventral polarity. Active ndl protease is generated by autoproteolytic cleavage of a zymogen form. This activation is independent of the other known proteases involved in establishment of dorsoventral polarity and appears to occur symmetrically on the surface of the embryo.

(LeMosy et al., 1998)

A follicular cell marker system that yields a visible phenotype within the mature egg shell allows direct comparison of a clone and its effect on the dorsal ventral pattern of the embryos. Follicular epithelium has a lack of a localised requirement for ndl protease activity.

(Nilson and Schupbach, 1998)

Phenotypic and genetic analysis reveals that ndl has two distinct roles important for embryogenesis: one in establishing dorsoventral polarity, the other in ensuring structural integrity of the egg.

(Hong and Hashimoto, 1996)

ndl is a somatic-dependent gene required to activate and localise the serine protease cascade that produces Tls ligand.

(Hong and Hashimoto, 1995)

Local activation of the protease cascade within the extra embryonic perivitelline compartment that defines embryonic dorsoventral polarity depends on a positional cue that is laid down during oogenesis outside the oocyte, ndl encodes an essential component of this cue. ndl function is required during oogenesis and also during the early stages of embryogenesis.

(Hong and Hashimoto, 1995)

Double mutant combinations of ndl with ea alleles demonstrate that spatial regulation of ea activity by localized zymogen activation is a key initial event in defining the polarity of the dorsal-ventral embryonic pattern.

(Chasan et al., 1992)

Strong dorsalizing mutants of ndl, snk, gd or pip have wild type axial ratios in pupae.

(Letsou et al., 1991)

Analysis of mosaic females indicates that the expression of ndl, pip and wbl is required in the somatic tissue, in the follicle cells that surround the oocyte, for the production of embryos with the correct dorsoventral axis.

(Stein et al., 1991)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: ndl CG10129

(Gabrielian, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

CG10129

(National Institute of Genetics Fly Stocks, 2023-, Keleman et al., 2009.8.5, Perkins et al., 2009, Shah et al., 2008, Fakhouri et al., 2006)

Ndl

(Roth, 2023, Schloop et al., 2020, Cao and Jiang, 2018, Mbodj et al., 2013, Kim et al., 2002)

SP89

(Cao and Jiang, 2018, Ross et al., 2003)

mat(3)2

mel(3)5

ndl

(Evans et al., 2022, Kumar et al., 2021, York-Andersen et al., 2021, Alpar et al., 2018, Mineo et al., 2017, Kang et al., 2016, Gene Disruption Project members, 2015-, Martins et al., 2014, Short and Lazzaro, 2013, Habermann et al., 2012, Shapira et al., 2011, Cho et al., 2010, Steen et al., 2010, Stein et al., 2008)

nud

(Ray and Schupbach, 1996)

Name Synonyms

Nudel

(York-Andersen et al., 2021, Lindsay and Wasserman, 2014, Puram and Bonni, 2013, Shah et al., 2008, Tian and LeMosy, 2008, Fakhouri et al., 2006, D'Alterio et al., 2005)

nudel

(Nakano et al., 2023, Nüsslein-Volhard, 2022, Cho et al., 2010, Shah et al., 2008, Stein et al., 2008)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 34 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38738

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

U29153

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

P98159

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

P98159

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

P98159

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

38738/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0002926

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0002926

FlyMine - An integrated database for Drosophila genomics

FBgn0002926

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG10129

MARRVEL_MODEL - MARRVEL (model organism gene)

38738

MEROPS - An information resource for peptidases (also termed proteases, proteinases and proteolytic enzymes) and the proteins that inhibit them.

S01.950

Linkouts

BioGRID - A database of protein and genetic interactions.

64189

DroID - A comprehensive database of gene and protein interactions.

FBgn0002926

DRSC - Results frm RNAi screens

FBgn0002926

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ndl_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0002926

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0002926

Flygut - An atlas of the Drosophila adult midgut

FBgn0002926

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC000870

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

38738

References (154)

The following external sites may use different assemblies or annotations than FlyBase.

-0.34

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