FlyBase Allele Report: Dmel\csw[hs.2sev.Tag:Myr(Src64B)]

General Information

Symbol

Dmel\csw^{hs.2sev.Tag:Myr(Src64B)}

Species

D. melanogaster

Name

FlyBase ID

FBal0049399

Feature type

allele

Associated gene

Dmel\csw

Associated Insertion(s)

Carried in Construct

P{sevhs-Myr-csw}

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Allard et al., 1998, Allard et al., 1996)

Progenitor genotype

Carried in construct

P{sevhs-Myr-csw}

Cytology

Description

Construct: Coding region of the first 90 amino acids from Src64B is fused in frame to the coding region for csw amino terminus. Mutant protein is expressed under the transcriptional control of an Hsp70 promoter and two copies of the sev enhancer.

(Allard et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

sev

Encoded product / tool

csw

Tagged with

Tag:Myr(Src64B)

membrane protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Smith et al., 2002)

Phenotype Manifest In

cone cell | precursor

(Allard et al., 1996)

eye

(Allard et al., 1996)

photoreceptor cell R7 | ectopic

(Smith et al., 2002)

photoreceptor cell R7 | increased number

(Jarvis et al., 2006)

Detailed Description

Statement

Reference

Flies that express one copy of csw::Src64B^hs.2sev in developing eyes often display extra R7 photoreceptor cells; while over 30% of such flies show the wild-type number of one R7 cell per ommatidium, over 30% show 2 per ommatidium, ~20% show 3 per ommatidium and a small number of flies show 4, 5, or even 6 R7 cells.

(Jarvis et al., 2006)

Flies carrying csw::Src64B^hs.2sev have an average of 1.2 ectopic R7 cells per ommatidium.

(Smith et al., 2002)

Eyes of transformed flies are slightly roughened. Roughness is due to the recruitment of cone cell precursors to the R7 fate. R7 cells can even be induced in ommatidia that otherwise lack R7 function. Ras85D^N17.hs.2sev completely blocks the ability of csw::Src64B^hs.2sev to induce R7 development.

(Allard et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

csw^{hs.2sev.Tag:Myr(Src64B)} has visible phenotype, suppressible by mask[+]/mask^5.8

(Smith et al., 2002)

csw^{hs.2sev.Tag:Myr(Src64B)} has visible phenotype, suppressible by mask[+]/mask^10.22

(Smith et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

csw^{hs.2sev.Tag:Myr(Src64B)} has photoreceptor cell R7 | increased number phenotype, enhanceable by sty[+]/sty^Δ5

(Jarvis et al., 2006)

Suppressed by

Statement

Reference

csw^{hs.2sev.Tag:Myr(Src64B)} has photoreceptor cell R7 | ectopic phenotype, suppressible by mask[+]/mask^10.22

(Smith et al., 2002)

csw^{hs.2sev.Tag:Myr(Src64B)} has photoreceptor cell R7 | ectopic phenotype, suppressible by mask[+]/mask^5.8

(Smith et al., 2002)

csw^{hs.2sev.Tag:Myr(Src64B)} has phenotype, suppressible by dos^1.46

(Herbst et al., 1996)

csw^{hs.2sev.Tag:Myr(Src64B)} has phenotype, suppressible by dos^2.42

(Herbst et al., 1996)

csw^{hs.2sev.Tag:Myr(Src64B)} has phenotype, suppressible by dos^unspecified

(Herbst et al., 1996)

Suppressor of

Statement

Reference

csw^{hs.2sev.Tag:Myr(Src64B)} is a suppressor of phenotype of csw^13-87

(Allard et al., 1998)

csw^{hs.2sev.Tag:Myr(Src64B)} is a suppressor of phenotype of csw^{G547E.hs.2sev}

(Allard et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Flies that express one copy of csw::Src64B^hs.2sev in developing eyes with a sty^Δ5/+ background show an enhancement of the extra R7 photoreceptor cell phenotype seen when csw::Src64B^hs.2sev is expressed in a wild-type background. A higher proportion of the csw::Src64B^hs.2sev/+; sty^Δ5/+ flies show 3, 4, 5,or 6 R7 photoreceptor cells per ommatidium.

(Jarvis et al., 2006)

mask^10.22 dominantly suppresses the ectopic R7 phenotype caused by csw::Src64B^hs.2sev; the double mutant flies have an average of 0.6 ectopic R7 cells per ommatidium. mask^5.8 dominantly suppresses the ectopic R7 phenotype caused by csw::Src64B^hs.2sev; the double mutant flies have an average of 0.8 ectopic R7 cells per ommatidium.

(Smith et al., 2002)

Rescues the zygotic lethality of csw^13-87/Y males.

(Allard et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Membrane targetted form of csw.

(Herbst et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

csw::Src64B^hs.2sev

csw^{hs.2sev.Tag:Myr(Src64B)}

csw^src90

Name Synonyms

Secondary FlyBase IDs

FBal0051682

References (5)

Report Sections

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