FlyBase Allele Report: Dmel\pyd[hs.PT]

General Information

Symbol

Dmel\pyd^hs.PT

Species

D. melanogaster

Name

heat shock construct of Takahisa

FlyBase ID

FBal0051069

Feature type

allele

Associated gene

Dmel\pyd

Associated Insertion(s)

Carried in Construct

P{hs-pyd.T}

Key Links

Transgenic product class

cDNA

(Takahisa et al., 1996)

wild_type

(Takahisa et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Takahisa et al., 1996)

wild_type

(Takahisa et al., 1996)

Mutagen

in vitro construct

(Takahisa et al., 1996)

Progenitor genotype

Carried in construct

P{hs-pyd.T}

Cytology

Description

Expression of a 5kb fragment containing the pyd cDNA is driven by an Hsp70 promoter.

(Takahisa et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

pyd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

wild-type

(Wei and Ellis, 2001, Ellis, 1998.6.17, Takahisa et al., 1996)

Phenotype Manifest In

interommatidial precursor cell | pupal stage

(Seppa et al., 2008)

secondary pigment cell

(Seppa et al., 2008)

tertiary pigment cell | pupal stage | increased number

(Seppa et al., 2008)

Detailed Description

Statement

Reference

Expression of pyd^hs.PT results in mild inter-ommatidial precursor cell (IPC) patterning errors. IPCs are seen to cluster around the inter-ommatidial bristles bristles and three cells are commonly observed to share the 3[o] cell niche equally, where normally a single cell is found in this position. Sometimes a 2[o] cell extends to cover both the 2[o] and 3[o] niches.

(Seppa et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

pyd^hs.PT/pyd^6-.hs rescues pyd^C5/pyd^J14

(Wei and Ellis, 2001)

pyd^hs.PT rescues pyd^C5

(Ellis, 1998.6.17)

pyd^hs.PT rescues pyd^tam

(Takahisa et al., 1996)

Partially rescues

pyd^hs.PT partially rescues pyd^C5/pyd^J14

(Wei and Ellis, 2001)

Comments

Partially rescues the extra bristle phenotype seen in pyd^C5/pyd^J14 flies, when exposed to heat shock at 37^oC and rescues the lethality phenotype seen in pyd^C5/pyd^C5 flies. The simultaneous expression of pyd^6-.hs and pyd^hs.PT produces a more complete rescue of the extra bristle phenotype seen in pyd^C5/pyd^J14 flies.

(Wei and Ellis, 2001)

pyd^hs.PT rescues the extra bristle phenotype of pyd^tam.

(Takahisa et al., 1996)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pyd^hs.PT

Name Synonyms

heat shock construct of Takahisa

(Takahisa et al., 1996)

Secondary FlyBase IDs

References (4)

Report Sections

Open Close