At permissive temperatures of 18-20^oC, put¹³⁵/put⁶² embryos do not show significant axon guidance defects. At 23-25^oC, these embryos show some guidance defects, affecting the intersegmental nerve and the SNa and SNb. At temperatures above 25^oC, embryos of this genotype exhibit gross head involution and dorsal closure defects.

Homozygous embryos maintained at the restrictive temperature (25^oC) have a single, large hole in the dorsal epidermis.

At 25^oC, 17% of homozygous embryos have a strong dorsal-open phenotype (large holes in the dorsal cuticle), 42% have an intermediate phenotype (the dorsal cuticle is noticeably reduced, and there is increased curvature in the abdominal segments, resulting in a "tail-up" phenotype) and 42% have a weak phenotype (dorsal closure is completed and there are minor defects in the dorsal cuticle). put¹⁰/put⁶² adults are viable, and sometimes show defects in wing venation, with ectopic venation stemming from the posterior crossvein into the second posterior cell. put⁶²/put¹³⁵ flies are inviable at 25^oC and fully viable at 18^oC. put⁶²/put¹³⁵ flies maintained at 18^oC throughout embryogenesis and then shifted to 25^oC show a marked reduction in viability and are grossly deformed. These animals all have notal defects, usually medial notal clefts, and have leg defects in 99% of cases, which include truncations, bifurcations and abnormal twists. Distal pattern elements are usually deleted in at least one limb. Duplications of sex combs are often seen on the forelegs. 97% of these flies have gross eye and antennal defects. The eyes are highly disorganised and have a reduced number of ommatidia and bristles. Antennal defects include duplications and deletions of distal pattern elements.

Produces a higher number of clones and larger clones than put¹³⁵. Clones induced in 2nd instar larvae that arose in ventral regions or were proximal to the distal tibia have no effect on bristle pattern. However clones close on the extreme dorsal surface of distal leg segments show a loss of dorsal structures, duplication of ventral structures and dorsal bifurcations. Duplicated structures frequently contain wild-type bristles. Clones in adult wings cause reduced wing size, ectopic vein formation, blisters and ectopic wing margin structures.

put⁶² is a non-enhancer of partially lethal - majority die phenotype of Neto¹⁰⁹

put⁶²/wit^G15 is a non-enhancer of partially lethal - majority die phenotype of Neto¹⁰⁹