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General Information
Symbol
Dmel\inscUAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kraut
FlyBase ID
FBal0051487
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-insc
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a HindIII-NotI fragment of insc cDNA.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In the majority of inscScer\UAS.cKa, Scer\GAL4sca-P309 telophase pI cells (13/17), the spindle becomes asymmetric with an anterior bias with the distance from the cleavage furrow to the anterior centrosome being greater than that to the posterior centrosome. In inscScer\UAS.cKa, Scer\GAL4sca-P309 animals, the mitotic spindle reorients to align with the A/B axis in ventral epithelial cells of the neuroectoderm. The apical spindle arm is preferentially extended, as judged by the position of the centrosomes relative to the cleavage furrow, the spindle is also displaced toward the basal cortex (ratio of longer half of spindle arm: shorter half of spindle arm = 1.39 plus or minus 0.25, n = 9), and a larger apical daughter is produced.

When inscScer\UAS.cKa is driven by Scer\GAL4neur-P72, the polarity of the pI sensory organ precursor cell is reversed.

Scer\GAL4h-1J3 induced expression in ectodermal cells outside the procephalic neurogenic region (PNR) leads to the reorientation of the mitotic spindle.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The spindle misorientation phenotype in Df(2L)TE35BC-3 embryos is largely but not completely corrected by expression of inscScer\UAS.cKa by Scer\GAL4sca-537.4.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
inscScer\UAS.cKa
inscUAS.cKa
nemUAS.cKa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Kraut
Secondary FlyBase IDs
    References (9)