FlyBase Allele Report: Dmel\Thor[k13517]

General Information

Symbol

Dmel\Thor^k13517

Species

D. melanogaster

Name

FlyBase ID

FBal0051843

Feature type

allele

Associated gene

Dmel\Thor

Associated Insertion(s)

P{lacW}Thor^k13517

Carried in Construct

Also Known As

Thor¹, 4E-BP-lacZ, thor-lacZ

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Tettweiler et al., 2005, Bernal and Kimbrell, 2000)

Mutagen

P-element activity

(Bernal and Kimbrell, 2000, Rodriguez et al., 1996)

Progenitor genotype

Associated Insertion(s)

P{lacW}Thor^k13517

Cytology

Description

The P{lacW}Thor^k13517 insertion is adjacent to the TATA box of the Thor gene, separating the coding region from the promoter region.

(Bernal and Kimbrell, 2000)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:3,478,408..3,478,615 [+]

(Lee et al., 2010, Tettweiler et al., 2005, Bernal and Kimbrell, 2000, Rodriguez et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response

(Bernal and Kimbrell, 2000)

abnormal stress response

(Zid et al., 2009)

chemical sensitive

(Imai et al., 2008)

fertile

(Bernal and Kimbrell, 2000)

short lived

(Zid et al., 2009)

viable

(Bernal and Kimbrell, 2000, Rodriguez et al., 1996)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous Thor^k13517 mutants exhibit wild-type synaptic differentiation.

(Lee et al., 2010)

Thor^k13517 mutant flies show a diminished change in lifespan when nutrient conditions are varied, however they show similar reductions in egg production as in controls.

(Zid et al., 2009)

Mutants show higher sensitivity to paraquat and to hydrogen peroxide than control flies.

(Imai et al., 2008)

Has no effect on ommatidial number compared to wild type flies.

(Junger et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Thor^k13517 is a suppressor of abnormal neuroanatomy phenotype of Lrrk^e03680

(Lee et al., 2010)

NOT Suppressor of

Statement

Reference

Thor^k13517/Thor^k13517 is a non-suppressor of decreased occurrence of cell division | somatic clone | third instar larval stage phenotype of mTor^ΔP

(Kim et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

Thor^k13517 is a suppressor of neuromuscular junction phenotype of Lrrk^e03680

(Lee et al., 2010)

Thor^k13517 is a suppressor of NMJ bouton phenotype of Lrrk^e03680

(Lee et al., 2010)

NOT Suppressor of

Statement

Reference

Thor^k13517/Thor^k13517 is a non-suppressor of eye disc | somatic clone | third instar larval stage phenotype of mTor^ΔP

(Kim et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Additional clonal homozygosity for Thor^k13517 does not suppress the delay in G1/S transition during the second mitotic wave in third instar larval eye discs displayed by Tor^ΔP homozygous somatic clones.

(Kim et al., 2017)

The synaptic overgrowth phenotype seen in Lrrk^e03680 homozygotes is suppressed by the simultaneous loss of Thor in a homozygous Thor^k13517 background.

The significant increase of futsch-positive MT loops seen in Lrrk^e03680 mutants is unaffected by a Thor^k13517 mutant background.

(Lee et al., 2010)

Slightly but significantly suppresses the reduced cell number phenotype of Akt1 mutants in a dose dependent manner.

(Junger et al., 2003)

4 days after infection with S. epidermis only 46% of mutant flies survive, compared to over 80% in wild-type. 4 days after infection with M. roseus, 11% of mutant flies survive as compared to 46% in wild-type.

(Bernal and Kimbrell, 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Thor^k13517 is rescued by Scer\GAL4^da.G32/Thor^UAS.cMa

(Zid et al., 2009)

Thor^k13517 is rescued by Scer\GAL4^da.G32/Thor^UAS.cMa

(Imai et al., 2008)

Comments

Ubiquitous expression of Thor^Scer\UAS.cMa under the control of Scer\GAL4^da.G32 rescues the dietary restriction phenotype of Thor^k13517 mutants from 8% to 37% lifespan extension in males and from 13% to 48% in females, similar to controls.

(Zid et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9558

y¹ w^*; P{lacW}Thor^k13517

Notes on Origin

Discoverer

Comments

Level of Ecol\lacZ expression and viability of adults is altered by bacterial infection of adults.

(Rodriguez et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

135/17

(Rodriguez et al., 1996)

4E-BP^-

(Zid et al., 2009)

4E-BP^k13517

(Kim et al., 2017)

Phas1^k13517

Thor¹

(Nowak et al., 2013, Lee et al., 2010, Tettweiler et al., 2005, Reiling and Hafen, 2004, Junger et al., 2003, Bernal and Kimbrell, 2000)

Thor^k13517

d4E-BP^-

(Imai et al., 2008)

thor¹

(Lee et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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