FlyBase Allele Report: Dmel\E(spl)m8-HLH[UAS.cNa]

General Information

Symbol

Dmel\E(spl)m8-HLH^UAS.cNa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Nakao

FlyBase ID

FBal0052455

Feature type

allele

Associated gene

Dmel\E(spl)m8-HLH

Associated Insertion(s)

Carried in Construct

P{UAS-E(spl)m8-HLH.N}

Also Known As

UAS-m8

Key Links

Transgenic product class

wild_type

(Nakao and Campos-Ortega, 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Nakao and Campos-Ortega, 1996)

Mutagen

in vitro construct

(Nakao and Campos-Ortega, 1996)

Progenitor genotype

Carried in construct

P{UAS-E(spl)m8-HLH.N}

Cytology

Description

UASt regulatory sequences drive expression of the E(spl)m8-HLH coding region.

(Nakao and Campos-Ortega, 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

E(spl)m8-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

central nervous system, with Scer\GAL4^da.G32

(Nakao and Campos-Ortega, 1996)

macrochaeta, with Scer\GAL4^da.G32

(Nakao and Campos-Ortega, 1996)

macrochaeta, with Scer\GAL4^sca-537.4

(Nakao and Campos-Ortega, 1996)

microchaeta, with Scer\GAL4^da.G32

(Nakao and Campos-Ortega, 1996)

microchaeta, with Scer\GAL4^sca-537.4

(Nakao and Campos-Ortega, 1996)

peripheral nervous system, with Scer\GAL4^da.G32

(Nakao and Campos-Ortega, 1996)

scutellar bristle, with Scer\GAL4^455.2

(Ligoxygakis et al., 1999)

scutum & macrochaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

scutum & macrochaeta, with Scer\GAL4^h-1J3

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

wing margin bristle, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

wing vein, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

Detailed Description

Statement

Reference

Flies expressing E(spl)^Scer\UAS.cNa under the control of Scer\GAL4^32B show loss of wing veins. Loss of notum microchaetae and wing margin bristles is seen. Flies expressing E(spl)^Scer\UAS.cNa under the control of Scer\GAL4^ap-md544 show complete loss of notum microchaetae and macrochaetae, except at the very anterior edge of the notum. Flies expressing E(spl)^Scer\UAS.cNa under the control of Scer\GAL4^455.2 show severe loss of scutellar bristles. Flies expressing E(spl)^Scer\UAS.cNa under the control of Scer\GAL4^h-1J3 show loss of notum macrochaetae.

(Ligoxygakis et al., 1999)

When expressed under the influence of Scer\GAL4^da.G32, E(spl)^Scer\UAS.cNa can cause central and peripheral neural hypoplasia, accompanied by increased cell death. There are no obvious epidermal effects on these embryos. SI and SII neuroblast segregation is normal. The severity of this phenotype depends on the site of insertion of the P{UAS-E(spl).N} element. When expressed under the influence of Scer\GAL4^sca-537.4, E(spl)^Scer\UAS.cNa can cause defects in the patterns of bristles. Pupal lethal lines from Scer\GAL4^da.G32 or Scer\GAL4^sca-537.4 lack almost all macro and microchaetae. Notal chaetae are particularly affected in E(spl)^Scer\UAS.cNa.

(Nakao and Campos-Ortega, 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Scer\GAL4^32B/E(spl)m8-HLH^UAS.cNa is an enhancer of visible | recessive | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Phenotype Manifest In

Enhancer of

Statement

Reference

Scer\GAL4^32B/E(spl)m8-HLH^UAS.cNa is an enhancer of wing | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Suppressor of

Statement

Reference

Scer\GAL4^klu-G410/E(spl)m8-HLH^UAS.cNa is a suppressor of proneural cluster phenotype of Su(H)⁸/Su(H)²

(Klein et al., 2000)

NOT Suppressor of

Statement

Reference

Scer\GAL4^klu-G410/E(spl)m8-HLH^UAS.cNa is a non-suppressor of wing disc phenotype of Su(H)⁸/Su(H)²

(Klein et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

E(spl)^Scer\UAS.cNa expressed under the control of Scer\GAL4^32B enhances the wing notching seen in Df(1)N-54l9 heterozygotes. Expression of E(spl)^Scer\UAS.cNa under the control of Scer\GAL4^32B enhances the scalloped wing phenotype seen in hemizygous N^l1N-ts1 flies shifted to the non-permissive temperature at the early third larval instar stage (72-84 hours after egg laying). Expression of E(spl)^Scer\UAS.cNa under the control of Scer\GAL4^32B has no effect on the nicked wing phenotype seen in hemizygous N^l1N-ts1 flies shifted to the non-permissive temperature at the mid third larval instar stage (96-108 hours after egg laying).

(Ligoxygakis et al., 1999)

The phenotype caused by E(spl)^Scer\UAS.cNa expressed under the influence of Scer\GAL4^da.G32 is dramatically enhanced if the flies also carry P{HLHm5-UAST}. Expression under the influence of Scer\GAL4^da.G32 attenuates the mutant phenotype of Df(3R)gro^b32.2. Males hemizygous for a deficiency for the ac-sc complex in which Scer\GAL4^da.G32 drives the expression of E(spl)^Scer\UAS.cNa are almost completely aneural. When E(spl)^Scer\UAS.cNa and l(1)sc^Scer\UAS.cCa are simultaneously driven by Scer\GAL4^da.G32, neural hypoplasia effects are much weaker than those caused by E(spl)^Scer\UAS.cNa alone, especially in the PNS. When E(spl)^Scer\UAS.cNa and l(1)sc^Scer\UAS.cCa are simultaneously driven by Scer\GAL4^sca-537.4, ectopic sense organs caused by l(1)sc^Scer\UAS.cCa are much reduced. Simultaneous expression of E(spl)^Scer\UAS.cNa or HLHm5^Scer\UAS.cNa with E(spl)^{1,bd-.Scer\UAS} driven by Scer\GAL4^da.G32 causes severe neural hypoplasia in embryos and adults, and reduces the level of neural hyperplasia in Df(3R)gro^b32.2.

(Nakao and Campos-Ortega, 1996)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26873

w^*; P{UAS-E(spl)m8-HLH.N}8602/CyO, P{elav-lacZ.H}CG17646^YH2

Notes on Origin

Discoverer

Comments

The relative ability of UAS constructs of the E(spl) complex to induce wing vein deletion when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): HLHmβ^{Scer\UAS.cdCa} = HLHmγ^Scer\UAS.cLa > E(spl)^Scer\UAS.cNa = HLHm7^{Scer\UAS.cdCa} > HLHmδ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of notum microchaetae when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} >> HLHmγ^Scer\UAS.cLa = HLHmβ^{Scer\UAS.cdCa} = HLHm3^Scer\UAS.cLa > HLHmδ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of wing margin bristles when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): HLHm7^{Scer\UAS.cdCa} > E(spl)^Scer\UAS.cNa > HLHmγ^Scer\UAS.cLa > HLHmδ^{Scer\UAS.cdCa} > HLHmβ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of notum macrochaetae when expressed under the control of Scer\GAL4^h-1J3 is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} > HLHmβ^{Scer\UAS.cdCa} > HLHmδ^{Scer\UAS.cdCa} > HLHmγ^Scer\UAS.cLa = HLHm3^Scer\UAS.cLa.

(Ligoxygakis et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

E(spl)^Scer\UAS.cNa

E(spl)^UAS.cNa

E(spl)m8-HLH^Scer\UAS.cNa

E(spl)m8-HLH^UAS.cNa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Nakao

(Nakao and Campos-Ortega, 1996)

Secondary FlyBase IDs

References (6)

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