FlyBase Allele Report: Dmel\ine[3]

General Information

Symbol

Dmel\ine³

Species

D. melanogaster

Name

FlyBase ID

FBal0057249

Feature type

allele

Associated gene

Dmel\ine

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

gamma ray

Nature of the Allele

Allele class

Mutagen

gamma ray

(Soehnge et al., 1996)

Progenitor genotype

Cytology

Description

2.4kb deletion, removing sequences from codon 293 to approximately 700bp beyond the transcription stop site.

(Soehnge et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:4,466,960..4,469,360 [-]

Comment:

~2.4 kb deletion beginning in the ine coding sequence. The endpoint within ine was mapped exactly. The boundary 3' of ine is approximate.

(Soehnge et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response

(Luan et al., 2015)

chemical sensitive

(Huang et al., 2002)

Phenotype Manifest In

Detailed Description

Statement

Reference

In contrast to control flies, ine³ mutant flies die within 10 days on hypertonic media. As in wild type, no tissue damage is observed in the anterior hindgut.

When maintained on hypertonic media, the hemolymph volume and total body water content of ine³ mutant flies declines dramatically compared to controls. Hemolymph volume and total body water content of ine³ mutant flies are unaffected on normal medium.

The median survival rate of ine³ mutant flies in response to lack of water is reduced compared to wild type controls. Survival rate following starvation (in the presence of sufficient water) is unchanged compared to controls.

(Luan et al., 2015)

ine³ flies show significantly greater lethality than wild-type flies when maintained for 4 days on food medium containing 0.2M NaCl. The flies show essentially complete inviability after 4 days on food medium containing 0.4M NaCl (in contrast to wild type flies, where 90% survive). Viability after 4 days on food medium containing 0M or 0.1M NaCl is similar to wild type. The flies also show increased sensitivity to KCl and sorbitol compared to wild-type flies. Mutant flies show normal sensitivity to heat shock stresses.

(Huang et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ine³ is rescued by ine^RA.UAS/Scer\GAL4^Irk2.PL

(Luan et al., 2015)

ine³ is rescued by ine^RB.UAS.cLa/Scer\GAL4^Irk2.PL

(Luan et al., 2015)

ine³ is rescued by ine^RB.UAS/Scer\GAL4^hs.PB

(Huang et al., 2002)

Not rescued by

ine³ is not rescued by Scer\GAL4^elav.PU/ine^RA.UAS

(Luan et al., 2015)

ine³ is not rescued by ine^RA.UAS/Scer\GAL4^repo.PU

(Luan et al., 2015)

ine³ is not rescued by ine^RB.UAS.cLa/Scer\GAL4^elav.PU

(Luan et al., 2015)

ine³ is not rescued by ine^RB.UAS.cLa/Scer\GAL4^repo.PU

(Luan et al., 2015)

Comments

Expression of ine^RA.Scer\UAS in the hindgut under the control of Scer\GAL4^Irk2.PL rescues the lethality seen when ine³ mutant flies are raised on hypertonic medium. Expressing ine^RA.Scer\UAS in neurons (Scer\GAL4^elav.PU) or glia (Scer\GAL4^repo.PU) does not rescue the lethality.

Expression of ine^RB.Scer\UAS in the hindgut under the control of Scer\GAL4^Irk2.PL rescues the lethality seen when ine³ mutant flies are raised on hypertonic medium. Expressing ine^RB.Scer\UAS in neurons (Scer\GAL4^elav.PU) or glia (Scer\GAL4^repo.PU) does not rescue the lethality.

Expression of ine^RA.Scer\UAS in the hindgut under the control of Scer\GAL4^Irk2.PL completely rescues the reduction in hemolymph volume and total body water content seen when ine³ mutant flies are kept on a hypertonic medium.

(Luan et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ine³

(Borycz et al., 2018, Luan et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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