FlyBase Gene Report: Dmel\ine

General Information

Symbol

Dmel\ine

Species

D. melanogaster

Name

inebriated

Annotation Symbol

CG15444

Feature Type

protein_coding_gene

FlyBase ID

FBgn0011603

Gene Model Status

Current

Stock Availability

20 publicly available

Gene Summary

inebriated (ine) encodes a protein that belongs to the SLC6A family of neurotransmitter transporters. It is involved in the control of photoreceptor potential, perineurial glial growth and the osmotic stress response. It also acts in the peripheral glia to control motor neuron excitability in third instar larvae. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

rosA

Key Links

Genomic Location

Cytogenetic map

24F4-24F4

Sequence location

2L:4,468,000..4,477,022 [-]

Recombination map

2-12

RefSeq locus

NT_033779 REGION:4468000..4477022

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (15 terms)

Molecular Function (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables osmosensor activity

inferred from mutant phenotype

(Huang et al., 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables amino acid:sodium symporter activity

inferred from biological aspect of ancestor with PANTHER:PTN000907238

(GO Reference Genome Project, 2011-)

enables neurotransmitter:sodium symporter activity

inferred from sequence or structural similarity with FLYBASE:SerT; FB:FBgn0010414

(Soehnge et al., 1996)

inferred from sequence or structural similarity with UniProtKB:P48065

(Soehnge et al., 1996)

inferred from sequence model

(Thimgan et al., 2006)

inferred from electronic annotation with InterPro:IPR002944

(InterPro Project Members, 2004-)

enables osmosensor activity

inferred from electronic annotation with InterPro:IPR002944

(InterPro Project Members, 2004-)

Biological Process (9 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in glial cell growth

inferred from mutant phenotype

(Yager et al., 2001)

involved_in multicellular organismal-level water homeostasis

inferred from mutant phenotype

(Luan et al., 2015)

involved_in perineurial glial growth

inferred from genetic interaction with FLYBASE:poe; FB:FBgn0011230

(Yager et al., 2001)

inferred from genetic interaction with FLYBASE:Nf1; FB:FBgn0015269

(Yager et al., 2001)

involved_in regulation of response to osmotic stress

inferred from mutant phenotype

(Huang et al., 2002)

involved_in response to water deprivation

inferred from mutant phenotype

(Luan et al., 2015)

involved_in transmission of nerve impulse

inferred from mutant phenotype

(Huang and Stern, 2002, Huang et al., 2002)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

involved_in glial cell growth

inferred from electronic annotation with InterPro:IPR002944

(InterPro Project Members, 2004-)

involved_in glycine import across plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000907238

(GO Reference Genome Project, 2011-)

involved_in neurotransmitter transport

inferred from electronic annotation with InterPro:IPR002944

(InterPro Project Members, 2004-)

inferred from sequence model

(Thimgan et al., 2006)

involved_in sodium ion transmembrane transport

inferred from biological aspect of ancestor with PANTHER:PTN000177662

(GO Reference Genome Project, 2011-)

involved_in transmission of nerve impulse

inferred from electronic annotation with InterPro:IPR002944

(InterPro Project Members, 2004-)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in basolateral plasma membrane

inferred from direct assay

(Luan et al., 2015)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in membrane

non-traceable author statement

(Huang and Stern, 2002)

inferred from sequence model

(Thimgan et al., 2006)

inferred from electronic annotation with InterPro:IPR000175, InterPro:IPR002944

(InterPro Project Members, 2004-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000177662

(GO Reference Genome Project, 2011-)

located_in plasma membrane

inferred from sequence or structural similarity with FLYBASE:SerT; FB:FBgn0010414

(Soehnge et al., 1996)

inferred from sequence or structural similarity with UniProtKB:P48065

(Soehnge et al., 1996)

Gene Group (FlyBase)

SLC6 FAMILY OF SODIUM/CHLORIDE-DEPENDENT NEUROTRANSMITTER TRANSPORTERS

Protein Family (UniProt)

Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. (Q9VR07)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

SLC6 FAMILY OF SODIUM/CHLORIDE-DEPENDENT NEUROTRANSMITTER TRANSPORTERS -: Solute carrier family 6 (SLC6) members are Na⁺/Cl^--dependent transporters of extracellular neurotransmitters, amino acids and osmolytes across the plasma membrane into the cytosol. (Adapted from FBrf0193583).

Protein Function (UniProtKB)

Plays a role in neuronal membrane excitation, important for normal response properties of the photoreceptor. Able to control excitability from either neurons or glia cells. Ine negatively regulates neuronal sodium channels. Controls neurotransmitter-mediated signaling pathways associated with the structure of the larval peripheral nerve, ine and eag control perineurial glial growth through partially redundant pathways. Isoform A and isoform B are both functional, although isoform A functions with greater efficiency. Has a role in osmolyte transport within the Malpighian tubule and hindgut.

(UniProt, Q9VR07)

Summary (Interactive Fly)

neurotransmitter:sodium symporter - mutations appear to cause increased excitability of the motor neuron by causing defective reuptake of a substrate neurotransmitter

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\ine for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9VR07)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.47

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

ine-RA

FBtr0077447

NM_057665

2272

658

ine-RB

FBtr0077446

NM_057664

3664

943

ine-RC

FBtr0077448

NM_175958

3452

943

ine-RE

FBtr0301407

NM_175959

3458

943

ine-RF

FBtr0301408

NM_001169400

3436

943

Additional Transcript Data and Comments

Reported size (kB)

2.6, 2.3 (northern blot)

(Soehnge et al., 1996)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

ine-PA

FBpp0077137

74.6

658

7.98

FBpp0077136

103.6

943

7.54

FBpp0077138

103.6

943

7.54

FBpp0290621

103.6

943

7.54

FBpp0290622

103.6

943

7.54

Q9VR07

NP_001162871

ACZ94162

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

943 aa isoforms: ine-PB, ine-PC, ine-PE, ine-PF

Additional Polypeptide Data and Comments

Reported size (kDa)

658 (aa); 75 (kD predicted)

(Soehnge et al., 1996)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\ine using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ine_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.97

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 5

organism | ubiquitous

(Soehnge et al., 1996)

embryonic stage 9 -- 17

inclusive hindgut primordium

(Soehnge et al., 1996)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

late embryonic stage

pharyngeal muscle cell

(Soehnge et al., 1996)

tracheal pit

(Soehnge et al., 1996)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

(Soehnge et al., 1996)

adult head

(Romero-Calderon et al., 2007)

Additional Descriptive Data

ine transcripts are uniformly distributed in cellular blastoderm embryos. During germ band extension, elevated levels are seen in the hindgut primordium. During germ band retraction, expression is observed in the midgut, Malpighian tubules, garland cells, anal plate, and foregut. ine is also expressed in the CNS in segmentally repeated cells flanking the ventral midline and in limited regions of the head. In late stage embryos, expression is also seen in tracheal pits and in pharyngeal muscle.

(Soehnge et al., 1996)

ine transcripts are expressed in adults.

(Soehnge et al., 1996)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in basolateral plasma membrane

inferred from direct assay

(Luan et al., 2015)

Expression Deduced from Reporters

Reporter: Mi{Trojan-GAL4.1}ine^{MI08294-TG4.1}

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult glial cell | subset

(Lee et al., 2018)

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\ine in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\ine

View exonic expression by tissue for Dmel\ine

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011603

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33659/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011603

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011603

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011603

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15444

Flygut - An atlas of the Drosophila adult midgut

FBgn0011603

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\ine

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 11 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of ine

Transgenic constructs containing regulatory region of ine

Aberrations (Deficiencies and Duplications) ( 7 )

Inferred from experimentation ( 7 )

Gene not disrupted in

Df(2L)ed1

(Stern and Ganetzky, 1992)

Df(2L)FIG4-Δ2

(Bharadwaj et al., 2016)

Gene disrupted in

Df(2L)ED252

(Mathew et al., 2009)

Df(2L)ed-dp

(Stern and Ganetzky, 1992)

Df(2L)Exel8011

(Ryder, 2004.4.26)

Df(2L)M24F-B

(Stern and Ganetzky, 1992)

Gene partially disrupted in

Df(2L)CRIMIC-CR70613

(Gene Disruption Project members, 2018-)

Inferred from location ( 4 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable

ine^d07155

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

ine^GD887

viable, with Scer\GAL4^Mef2.PR

ine^GD887

viable, with Scer\GAL4^pnr-MD237

ine^GD887

viable, with Scer\GAL4^tin.CΔ4

ine^GD887

Sterility

Allele

fertile

ine^d07155

Other Phenotypes

Allele

abnormal behavior

ine¹

abnormal neurophysiology | adult stage

ine^MI05077

abnormal phototaxis | adult stage

ine^MI05077

abnormal stress response

chemical sensitive

paralytic | heat sensitive, with Scer\GAL4^Gli-rL82

ine^RA.UAS

paralytic | heat sensitive, with Scer\GAL4^MZ1580

ine^RA.UAS

wild-type

ine^hs.P1

Phenotype manifest in

Allele

eye

ine^MI05077

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (19)

Hsap\SLC6A1

3 of 14

Yes

Hsap\SLC6A2

3 of 14

Yes

Hsap\SLC6A3

3 of 14

Yes

Hsap\SLC6A5

3 of 14

Yes

Hsap\SLC6A6

3 of 14

Yes

Hsap\SLC6A7

3 of 14

Yes

Hsap\SLC6A8

3 of 14

Yes

Hsap\SLC6A9

3 of 14

Yes

Hsap\SLC6A11

3 of 14

Yes

Hsap\SLC6A12

3 of 14

Yes

Hsap\SLC6A13

3 of 14

Yes

Hsap\SLC6A14

3 of 14

Yes

Hsap\SLC6A15

3 of 14

Yes

Hsap\SLC6A16

3 of 14

Yes

Hsap\SLC6A17

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (20)

Rnor\RGD1562492

3 of 14

Yes

Rnor\Slc6a1

3 of 14

Yes

Rnor\Slc6a2

3 of 14

Yes

Rnor\Slc6a3

3 of 14

Yes

Rnor\Slc6a5

3 of 14

Yes

Rnor\Slc6a6

3 of 14

Yes

Rnor\Slc6a7

3 of 14

Yes

Rnor\Slc6a9

3 of 14

Yes

Rnor\Slc6a11

3 of 14

Yes

Rnor\Slc6a12

3 of 14

Yes

Rnor\Slc6a13

3 of 14

Yes

Rnor\Slc6a14

3 of 14

Yes

Rnor\Slc6a17

3 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Rnor\Slc6a18

2 of 14

Rnor\Slc6a19

2 of 14

Rnor\Slc6a20

2 of 14

Mus musculus (laboratory mouse) (21)

Mmus\Slc6a1

3 of 14

Yes

Mmus\Slc6a2

3 of 14

Yes

Mmus\Slc6a3

3 of 14

Yes

Mmus\Slc6a4

3 of 14

Yes

Mmus\Slc6a6

3 of 14

Yes

Mmus\Slc6a7

3 of 14

Yes

Mmus\Slc6a8

3 of 14

Yes

Mmus\Slc6a9

3 of 14

Yes

Mmus\Slc6a11

3 of 14

Yes

Mmus\Slc6a12

3 of 14

Yes

Mmus\Slc6a13

3 of 14

Yes

Mmus\Slc6a14

3 of 14

Yes

Mmus\Slc6a15

3 of 14

Yes

Mmus\Slc6a5

2 of 14

Mmus\Slc6a16

2 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

Yes

Xenopus tropicalis (Western clawed frog) (26)

Xtro\slc6a8

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

Yes

Xtro\LOC100490052

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\tnip1

1 of 13

Xtro\XB5944457

1 of 13

Xtro\zmiz2

1 of 13

Danio rerio (Zebrafish) (35)

Drer\si:ch211-283g2.1

11 of 14

Yes

Drer\si:ch211-225b11.1

10 of 14

Yes

Drer\si:ch211-117c9.5

3 of 14

Drer\si:ch211-132b12.1

3 of 14

Drer\si:ch211-132b12.2

3 of 14

Drer\si:ch211-132b12.3

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

3 of 14

Drer\si:ch211-132b12.6

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (15)

Cele\snf-12

4 of 14

Yes

Cele\dat-1

3 of 14

Cele\mod-5

3 of 14

Cele\snf-1

3 of 14

Yes

Cele\snf-2

3 of 14

Cele\snf-3

3 of 14

Cele\snf-6

3 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (22)

Agam\AgaP_AGAP008940

11 of 12

Yes

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

3 of 12

2 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

Yes

Agam\AgaP_AGAP011279

2 of 12

Agam\AgaP_AGAP012565

2 of 12

Yes

Agam\AgaP_AGAP012626

2 of 12

Agam\AgaP_AGAP000494

1 of 12

Yes

Agam\AgaP_AGAP001194

1 of 12

Agam\AgaP_AGAP010862

1 of 12

Agam\AgaP_AGAP012290

1 of 12

Arabidopsis thaliana (thale-cress) (0)

Saccharomyces cerevisiae (Brewer's yeast) (0)

Schizosaccharomyces pombe (Fission yeast) (0)

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:ine. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (21)

5 of 13

5 of 13

5 of 13

5 of 13

5 of 13

DAT

5 of 13

Gat

5 of 13

List

5 of 13

NAAT1

5 of 13

Ntl

5 of 13

5 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

4 of 13

bdg

3 of 13

blot

3 of 13

CG10804

3 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 6 )

Human Ortholog

Disease

Evidence

References

SLC6A8; solute carrier family 6 member 8

model of cerebral creatine deficiency syndrome 1

IEA

(FlyBase, 2019-)

SLC6A3; solute carrier family 6 member 3

model of classic dopamine transporter deficiency syndrome

IEA

(FlyBase, 2019-)

SLC6A1; solute carrier family 6 member 1

model of myoclonic-atonic epilepsy

IEA

(FlyBase, 2019-)

SLC6A17; solute carrier family 6 member 17

model of autosomal recessive intellectual developmental disorder 48

IEA

(FlyBase, 2019-)

SLC6A5; solute carrier family 6 member 5

model of hyperekplexia 3

IEA

(FlyBase, 2019-)

SLC6A2; solute carrier family 6 member 2

model of postural orthostatic tachycardia syndrome

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

SLC6A1; solute carrier family 6 member 1

3 of 14

SOLUTE CARRIER FAMILY 6 (NEUROTRANSMITTER TRANSPORTER, GABA), MEMBER 1; SLC6A1

MYOCLONIC-ATONIC EPILEPSY; MAE

myoclonic-atonic epilepsy

SLC6A2; solute carrier family 6 member 2

3 of 14

163970

ORTHOSTATIC INTOLERANCE; ORSTI

postural orthostatic tachycardia syndrome

SLC6A3; solute carrier family 6 member 3

3 of 14

SOLUTE CARRIER FAMILY 6 (NEUROTRANSMITTER TRANSPORTER, DOPAMINE), MEMBER 3; SLC6A3

classic dopamine transporter deficiency syndrome

SLC6A5; solute carrier family 6 member 5

3 of 14

604159

HYPEREKPLEXIA 3; HKPX3

hyperekplexia 3

SLC6A6; solute carrier family 6 member 6

3 of 14

186854

HYPOTAURINEMIC RETINAL DEGENERATION AND CARDIOMYOPATHY; HTRDC

SLC6A7; solute carrier family 6 member 7

3 of 14

606205

SLC6A8; solute carrier family 6 member 8

3 of 14

300036

CEREBRAL CREATINE DEFICIENCY SYNDROME 1; CCDS1

cerebral creatine deficiency syndrome 1

SLC6A9; solute carrier family 6 member 9

3 of 14

601019

SLC6A11; solute carrier family 6 member 11

3 of 14

607952

SLC6A12; solute carrier family 6 member 12

3 of 14

603080

SLC6A13; solute carrier family 6 member 13

3 of 14

615097

SLC6A14; solute carrier family 6 member 14

3 of 14

300444

SLC6A15; solute carrier family 6 member 15

3 of 14

607971

SLC6A16; solute carrier family 6 member 16

3 of 14

607972

SLC6A17; solute carrier family 6 member 17

3 of 14

610299

INTELLECTUAL DEVELOPMENTAL DISORDER, AUTOSOMAL RECESSIVE 48; MRT48

autosomal recessive intellectual developmental disorder 48

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

ine - UQCR-6.4

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

ine

enhanceable

Nf1

(Yager et al., 2001)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Nf1

enhanceable

ine

(Yager et al., 2001)

para

enhanceable

ine

(Huang and Stern, 2002)

poe

enhanceable

ine

(Yager et al., 2001)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

59853

DroID - A comprehensive database of gene and protein interactions.

FBgn0011603

MIST (protein-protein) - An integrated Molecular Interaction Database

33659

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-12

Cytogenetic map

24F4-24F4

Sequence location

2L:4,468,000..4,477,022 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

24F4-24F4

Limits computationally determined from genome sequence between P{lacW}Tps1^k08903&P{EP}morgue^EP1184 and P{lacW}l(2)k10004^k10004&P{EP}CG3036^EP963

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

25A-25A

(Soehnge, 1996.8.8)

24F-25A

(determined by in situ hybridisation)

(Soehnge et al., 1995)

Experimentally Determined Recombination Data

Location

2-12

(FlyBase, 2016, Stern and Ganetzky, 1992)

2-13.5

(Comeron et al., 2012)

Left of (cM)

(Stern and Ganetzky, 1992)

Right of (cM)

(Stern and Ganetzky, 1992)

Notes

Stocks and Reagents

Stocks (20)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

40362

w¹¹¹⁸; P{GMR83E10-GAL4}attP2

40363

w¹¹¹⁸; P{GMR83E12-GAL4}attP2

40364

w¹¹¹⁸; P{GMR83F01-GAL4}attP2

54288

w¹¹¹⁸; P{GMR83E12-lexA}attP40

54296

w¹¹¹⁸; P{GMR83E10-lexA}attP40

94744

w¹¹¹⁸; PBac{GMR83E12-lexA}VK00027

38094

y¹ w^*; Mi{MIC}ine^MI05077

44762

y¹ w^*; Mi{MIC}ine^MI08294

15444R-1

15444R-2

v330044

v8880

More stocks available...

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (42)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

DPiM_HL05815

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0011603

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0011603

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Luan et al., 2015)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Mutants lacking both ine protein isoforms are hypersensitive to osmotic stress.

(Huang et al., 2002)

ine encodes a protein with a high degree of sequence similarity to members of the Na⁺/Cl^--dependent neurotransmitter transporter family.

(Soehnge et al., 1996)

Mutations of ine cause increased transmitter release due to prolonged depolarization at the nerve terminal.

(Soehnge et al., 1995)

In a Sh mutant background ine mutations display downturned wings and indented thorax. ine mutations increase neuronal membrane excitability and perhaps block a DDF-sensitive potassium channel.

(Stern and Ganetzky, 1992)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Name Synonyms

inebriated

(Luan et al., 2015, Xu et al., 2015, Gavin et al., 2007, Thimgan et al., 2006, Yager et al., 2001, Soehnge, 1996.8.8)

Secondary FlyBase IDs

FBgn0020253

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 56 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33659

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9VR07

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9VR07

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JHT2

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9VR07

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0011603

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

33659/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0011603

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0011603

FlyMine - An integrated database for Drosophila genomics

FBgn0011603

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG15444

MARRVEL_MODEL - MARRVEL (model organism gene)

33659

Linkouts

BioGRID - A database of protein and genetic interactions.

59853

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0011603

DroID - A comprehensive database of gene and protein interactions.

FBgn0011603

DRSC - Results frm RNAi screens

FBgn0011603

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

ine_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0011603

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0011603

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG15444

Flygut - An atlas of the Drosophila adult midgut

FBgn0011603

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC010681

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

33659

References (78)

The following external sites may use different assemblies or annotations than FlyBase.

-0.97

Report Sections