FlyBase Allele Report: Dmel\fru[w27]

General Information

Symbol

Dmel\fru^w27

Species

D. melanogaster

Name

FlyBase ID

FBal0057427

Feature type

allele

Associated gene

Dmel\fru

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Ryner et al., 1996)

Progenitor genotype

P{wA^R}fru^0-1

(Anand et al., 2001)

fru^0-1

(Ryner et al., 1996)

Caused by aberration

In(3LR)fru^w27

Cytology

Description

Southern analysis indicates a lesion within a 4kb HindIII fragment at the 5' end of the w gene in the P{wA^R}fru^0-1 element. RT-PCR analysis suggests that there is a breakpoint between the P2 and P3 promoters of fru.

(Anand et al., 2001)

Breakpoint maps to a 2.8kb BamHI-HindIII fragment at the 5' end of the w gene of the P{wA^R} insertion of fru^0-1.

(Ryner et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | male

(Ryner et al., 1996)

abnormal courtship behavior | male (with fru^0-1)

(Anand et al., 2001)

abnormal courtship behavior | male (with fru¹)

(Anand et al., 2001)

abnormal courtship behavior | male (with fru³)

(Anand et al., 2001)

abnormal courtship behavior | male (with fru^4-40)

(Anand et al., 2001)

abnormal courtship behavior | male (with fru⁴)

(Anand et al., 2001)

abnormal courtship behavior | male (with fru^w12)

(Anand et al., 2001)

abnormal neuroanatomy (with Df(3R)fru^w24)

(Anand et al., 2001)

abnormal neuroanatomy (with fru^w12)

(Anand et al., 2001)

abnormal song (with fru¹)

(Anand et al., 2001)

abnormal song (with fru³)

(Anand et al., 2001)

abnormal song (with fru^4-40)

(Anand et al., 2001)

abnormal song (with fru⁴)

(Anand et al., 2001)

decreased rate of movement | male (with fru^4-40)

(Anand et al., 2001)

decreased rate of movement | male (with fru^w12)

(Anand et al., 2001)

lethal (with Df(3R)Cha7)

(Anand et al., 2001)

lethal (with fru^sat15)

(Anand et al., 2001)

lethal (with fru^w9)

(Anand et al., 2001)

lethal | recessive

(Lee and Hall, 2001, Lee et al., 2000, Ryner et al., 1996)

lethal - all die before end of pupal stage (with Df(3R)fru^w24)

(Anand et al., 2001)

lethal - all die before end of pupal stage (with fru^AJ96u3)

(Song et al., 2002)

lethal - all die before end of pupal stage (with fru^w12)

(Anand et al., 2001)

lethal - all die before end of pupal stage | partially (with fru^w12)

(Lee et al., 2000)

male sterile (with fru^0-1)

(Anand et al., 2001)

male sterile (with fru¹)

(Anand et al., 2001)

male sterile (with fru³)

(Anand et al., 2001)

male sterile (with fru^4-40)

(Anand et al., 2001)

male sterile (with fru⁴)

(Anand et al., 2001)

male sterile (with fru^M5)

(Anand et al., 2001)

male sterile (with fru^w9)

(Anand et al., 2001)

male sterile (with fru^w12)

(Anand et al., 2001)

some die during pupal stage (with Df(3R)fru^w24)

(Anand et al., 2001)

some die during pupal stage (with fru^AJ96u3)

(Song et al., 2002)

some die during pupal stage (with fru^w12)

(Anand et al., 2001)

some die during pupal stage | partially (with fru^w12)

(Lee et al., 2000)

Phenotype Manifest In

adult abdomen & motor neuron (with Df(3R)fru^w24)

(Anand et al., 2001)

adult abdomen & motor neuron (with fru^w12)

(Anand et al., 2001)

anterior scutellar bristle (with fru^AJ96u3)

(Song et al., 2002)

leg joint (with Df(3R)fru^w24)

(Anand et al., 2001)

leg joint (with fru^AJ96u3)

(Song et al., 2002)

leg joint (with fru^w12)

(Anand et al., 2001)

macrochaeta | ectopic (with Df(3R)fru^w24)

(Anand et al., 2001)

macrochaeta | ectopic (with fru^w12)

(Anand et al., 2001)

muscle cell of muscle of Lawrence (with fru^0-1)

(Anand et al., 2001)

muscle cell of muscle of Lawrence (with fru¹)

(Anand et al., 2001)

muscle cell of muscle of Lawrence (with fru³)

(Anand et al., 2001)

muscle cell of muscle of Lawrence (with fru^4-40)

(Anand et al., 2001)

muscle cell of muscle of Lawrence (with fru⁴)

(Anand et al., 2001)

muscle cell of muscle of Lawrence (with fru^M5)

(Anand et al., 2001)

sternopleural bristle (with fru^AJ96u3)

(Song et al., 2002)

tibia (with fru^w12)

(Anand et al., 2001)

wing (with Df(3R)fru^w24)

(Anand et al., 2001)

wing (with fru^AJ96u3)

(Song et al., 2002)

wing (with fru^w12)

(Anand et al., 2001)

Detailed Description

Statement

Reference

53% of fru^AJ96u3/fru^w27 animals can emerge if dissected from the pupal case. These flies have a number of defects including uneverted discs, uninflated wings, abnormal wing position, defective leg joints and duplicated anterior scutellar or sternopleural bristles.

(Song et al., 2002)

1-2% fru^w12/fru^w27 adult escapers are seen. 5% fru^sat15/fru^w27 adult escapers are seen. 0.1% fru^w9/fru^w27 adult escapers are seen. 3% of fru^w27/fru^M5 males are fertile. Male-female courtship as measured by courtship index (CI) or wing extension index (WEI) is almost completely abolished in fru^w12/fru^w27 or fru^4-40/fru^w27 males. Male-female courtship as measured by courtship index (CI) or wing extension index (WEI) is almost completely abolished in fru³/fru^w27 or fru⁴/fru^w27 males. Male-female courtship as measured by wing extension index (WEI) is almost completely abolished in fru^0-1/fru^w27 males. fru¹/fru^w27 males show significantly more male-male courtship and less frequent male-female courtship than wild-type males. fru^w27/fru¹ or fru^w27/fru⁴ males show substantial chaining. fru^w27/fru^M5 or fru^w27/fru^4-40 males show substantial chaining. Heterozygous males generate a normal courtship song except that the width of the fast-Fournier transform is smaller than in wild-type males. fru³/fru^w27 and fru^4-40/fru^w27 males do not produce a courtship song even though they show a small amount of wing extension. fru¹/fru^w27 males have a courtship song with longer interpulse intervals, but essentially normal intrapulse frequencies, cycles per pulse and width of the fast-Fourier transform compared to control males. fru^w12/fru^w27 animals are able to emerge if dissected from the pupal case. These animals perform posteclosion behaviours such as wing and proboscis expansion but are unable to fully extend their wings, which project from the body at a 70-90^o angle. The tibial segment may be bent. Leg joint defects and duplicated bristles are seen. fru^w27/Df(3R)fru^w24 adults that have been dissected from the pupal case show minor external defects, such as uninflated wings, wings held at a 70-90^o angle to the body, leg joint defects and duplicated bristles. Abdominal motorneurons of fru^w12/fru^w27 animals produce fewer lateral terminal arborisations as they terminate on lateral muscles compared to wild type. Abdominal motorneurons of fru^w27/Df(3R)fru^w24 animals produce fewer terminal branches compared to wild type. Abdominal muscle innervation appears normal in fru^w27/fru^M5 adults. The number and pattern of divisions of larval neuroblasts in the thoracic ganglia or brain appears normal in fru^w27/Df(3R)fru^w24 animals.

(Anand et al., 2001)

fru^w27/fru^w12 mutants are nearly lethal during late pupal stages.

(Lee et al., 2000)

Transheterozygotes with fru^w12 or Df(3R)fru^w24 are lethal, 2% fru^w12/fru^w27 transheterozygotes survive to adulthood. fru^w27/Df(3R)Cha^M5 males barely court at all but show normal locomotor activity. Mutant combination shows some male-male chaining. Early and late steps of courtship are disrupted.

(Ryner et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66701

w^*; In(3LR)fru^w27, fru^w27/TM6B, Tb⁺

Notes on Origin

Discoverer

Selected as: white-eyed derivative of fru^0-1.

(Anand et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

fru^w27

(Lee et al., 2001)

w27

(Lee et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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