FlyBase Allele Report: Dmel\RpL14[1]

General Information

Symbol

Dmel\RpL14¹

Species

D. melanogaster

Name

FlyBase ID

FBal0058322

Feature type

allele

Associated gene

Dmel\RpL14

Associated Insertion(s)

P{lacW}RpL14¹

Carried in Construct

Also Known As

M(3)66D

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Bloomington Drosophila Stock Center, 1997.1.15, Saeboe-Larssen et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}RpL14¹

Cytology

Description

P{lacW} insertion 52bp upstream of the major transcription start site.

(Saeboe-Larssen et al., 1997)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of carcinoma

CEA with TSG101²

(Moberg et al., 2005)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death | larval stage

(Recasens-Alvarez et al., 2021)

lethal | recessive

(Saeboe-Larssen et al., 1997)

Minute

(Marygold et al., 2007, Enerly et al., 2003, Bloomington Drosophila Stock Center, 1997.1.15)

Phenotype Manifest In

chaeta

(Enerly et al., 2003)

imaginal disc | somatic clone

(Recasens-Alvarez et al., 2021)

macrochaeta

(Saeboe-Larssen et al., 1997)

scutellar bristle

(Marygold et al., 2007)

wing

(Coelho et al., 2005)

wing disc

(Coelho et al., 2005)

Detailed Description

Statement

Reference

RpL14¹ heterozygous larvae show increased apoptosis in imaginal discs.

(Recasens-Alvarez et al., 2021)

RpL14¹/+ flies have shorter and thinner scutellar bristles.

(Marygold et al., 2007)

RpL14¹ wings are significantly larger than wild-type flies. There is an increase in the level of apoptosis in the wing discs of RpL14¹/+ larvae.

(Coelho et al., 2005)

When somatic clones expressing RpL14^Scer\UAS.cMa (under the control of Scer\GAL4^dpp.blk1) are made in a RpL14¹ background, animals have wings that are the same size as controls.

(de la Cova et al., 2004)

Strong to extreme Minute phenotype.

(Bloomington Drosophila Stock Center, 1997.1.15)

Heterozygotes exhibit a strong Minute phenotype: delayed larval development, short and thin bristles and recessive lethality.

(Saeboe-Larssen et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

RpL14[+]/RpL14¹ is an enhancer of increased cell death phenotype of pix^L17

(Coelho et al., 2005)

Suppressor of

Statement

Reference

RpL14¹ is a suppressor | partially of decreased size | somatic clone phenotype of Noc1^RNAi.UAS.cUa, Scer\GAL4^Act.PU

(Destefanis et al., 2022)

Other

Statement

Reference

HM36^HM36, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM12^HM12, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM30^HM30, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM805^HM805, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM400^HM400, RpL14¹, Scer\GAL4^B11-98 has lethal phenotype

(Minye and Merriam, 2001)

HM160^HM160, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM160^HM160, RpL14¹, Scer\GAL4^B11-98 has abnormal body color phenotype

(Minye and Merriam, 2001)

HM501^HM501, RpL14¹, Scer\GAL4^B11-98 has lethal phenotype

(Minye and Merriam, 2001)

HM388^HM388, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM210^HM210, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM274^HM274, RpL14¹, Scer\GAL4^B11-98 has lethal phenotype

(Minye and Merriam, 2001)

HM431^HM431, RpL14¹, Scer\GAL4^B11-98 has lethal phenotype

(Minye and Merriam, 2001)

HM530^HM530, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM786^HM786, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM415^HM415, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM515^HM515, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM421^HM421, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM689^HM689, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM165^HM165, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM56^HM56, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM298^HM298, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM696^HM696, RpL14¹, Scer\GAL4^B11-98 has lethal phenotype

(Minye and Merriam, 2001)

HM17^HM17, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM122^HM122, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM745^HM745, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM617^HM617, RpL14¹, Scer\GAL4^B11-98 has partially lethal - majority die phenotype

(Minye and Merriam, 2001)

HM781^HM781, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM54^HM54, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM813^HM813, RpL14¹, Scer\GAL4^B11-98 has lethal phenotype

(Minye and Merriam, 2001)

HM840^HM840, RpL14¹, Scer\GAL4^B11-98 has lethal phenotype

(Minye and Merriam, 2001)

HM804^HM804, RpL14¹, Scer\GAL4^B11-98 has partially lethal - majority die phenotype

(Minye and Merriam, 2001)

HM385^HM385, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM801^HM801, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM857^HM857, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM304^HM304, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

HM505^HM505, RpL14¹, Scer\GAL4^B11-98 has visible phenotype

(Minye and Merriam, 2001)

Phenotype Manifest In

Suppressor of

Statement

Reference

RpL14¹ is a suppressor | partially of epithelial cell of wing disc | somatic clone phenotype of Noc1^RNAi.UAS.cUa, Scer\GAL4^Act.PU

(Destefanis et al., 2022)

Other

Statement

Reference

HM36^HM36, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM12^HM12, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM30^HM30, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM805^HM805, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM160^HM160, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM160^HM160, RpL14¹, Scer\GAL4^B11-98 has wing phenotype

(Minye and Merriam, 2001)

HM786^HM786, RpL14¹, Scer\GAL4^B11-98 has wing phenotype

(Minye and Merriam, 2001)

HM388^HM388, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM210^HM210, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM530^HM530, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM415^HM415, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM515^HM515, RpL14¹, Scer\GAL4^B11-98 has wing phenotype

(Minye and Merriam, 2001)

HM421^HM421, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM689^HM689, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM689^HM689, RpL14¹, Scer\GAL4^B11-98 has wing phenotype

(Minye and Merriam, 2001)

HM165^HM165, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM745^HM745, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM56^HM56, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM298^HM298, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM17^HM17, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM122^HM122, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM781^HM781, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM54^HM54, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM385^HM385, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM304^HM304, RpL14¹, Scer\GAL4^B11-98 has wing phenotype

(Minye and Merriam, 2001)

HM801^HM801, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

HM857^HM857, RpL14¹, Scer\GAL4^B11-98 has wing phenotype

(Minye and Merriam, 2001)

HM505^HM505, RpL14¹, Scer\GAL4^B11-98 has macrochaeta phenotype

(Minye and Merriam, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The differences in pix^L17 clone growth between the pouch and hinge are maintained, and even exaggerated, in a RpL14¹/+ background. Although apoptosis is similar to wild-type controls in pix^L17/+ wing discs, levels are enhanced in pix^L17/+, RpL14¹/+ double mutants.

(Coelho et al., 2005)

Expression of HM12^HM12 under the control of Scer\GAL4^B11-98 in a RpL14¹ background results in flies with very minute bristles.

Expression of HM17^HM17 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with short bristles.

Expression of HM30^HM30 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with extremely short bristles.

Expression of HM36^HM36 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with very minute bristles.

Expression of HM54^HM54 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with short bristles.

Expression of HM56^HM56 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with extremely short bristles.

Expression of HM122^HM122 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in absent bristles.

Body colour: Expression of HM160^HM160 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with yellow wings and yellow

bristles.

Expression of HM165^HM165 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies which have one small bristle

at a specific location on the body.

Expression of HM210^HM210 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in absent bristles.

Expression of HM298^HM298 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with very short bristles.

Expression of HM304^HM304 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with curly wings.

Expression of HM385^HM385 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in absent bristles.

Expression of HM388^HM388 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in absent bristles.

Expression of HM415^HM415 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in absent bristles.

Expression of HM421^HM421 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies in which bristles are all

present but do not grow beyond the thorax shell.

Expression of HM505^HM505 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in absent bristles.

Expression of HM515^HM515 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with wrinkled wings.

Expression of HM530^HM530 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with very minute bristles.

Expression of HM689^HM689 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with very minute bristles

and curly wings.

Expression of HM745^HM745 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with very short bristles.

Expression of HM781^HM781 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with very thin bristles.

Expression of HM786^HM786 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with wrinkled wings.

Expression of HM801^HM801 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with short bristles.

Expression of HM805^HM805 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies in which bristle growth

stops at the thorax shell line.

Expression of HM857^HM857 under the control of Scer\GAL4^B11-98

in a RpL14¹ background results in flies with wrinkled wings.

(Minye and Merriam, 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

RpL14¹ is partially rescued by RpL14^UAS.cMa/Scer\GAL4^unspecified

(de la Cova et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

2247

y¹ w^*; P{lacW}RpL14¹/TM2

Notes on Origin

Discoverer

S. Saebow-Larssen and A. Lambertsson.

(Bloomington Drosophila Stock Center, 1997.1.15)

Comments

P{lacW} insertion is within the joint promoter region between Nelf-E and RpL14 but only affects expression of RpL14. Remobilisation of the P{lacW} insertion reveals it is responsible for the mutant phenotype.

(Saeboe-Larssen et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

M(3)66, P[w⁺]

(Lambertsson et al., 1994)

M(3)66D

(de la Cova et al., 2004, Minye and Merriam, 2001)

M(3)66D¹

(Saeboe-Larssen et al., 1997)

M66D¹

(Coelho et al., 2005)

Minute(3)66D

(Destefanis et al., 2022)

RPL14¹

(Recasens-Alvarez et al., 2021)

RpL14¹

(Guo et al., 2019, Marygold et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (13)

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