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FB2026_01
,
released March 12, 2026
Amino acid replacement: A77T.
G8974056A
A77T | pix-PA; A77T | pix-PB
A77T
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
pixL35/pixL17 flies have a slow developmental time (12-15 days) compared to wild-type flies (10 days). Adult flies are smaller than wild type with a decreased wing area, slender, short thoracic macrochaetae and occasional eye roughening. pixL35/pixL17 wing discs display distinct patterns of elevated apoptosis; there are uniformly distributed clusters of apoptotic cells during the early to late mid third instar, followed by a transient period during the late third instar when apoptosis is intense in the wing pouch. Shortly before pupation, apoptosis is less intense and more uniformly distributed. The resulting adult wings are patterned normally. pixL17 clones generated during late wing disc growth are reduced in frequency and clone size compared with their wild-type sister clones. During the fast growth at the mid-third instar stage, pixL17 mutant clone size and frequency are greatly reduced within 29-30 min of clone of clone induction and clones are completely absent within 46 hours. Clone growth is poorer in the hinge than the pouch. During the slow growth phase, pixL17 clones grow better in the hinge, but growth in the pouch remains poor during part of the slow growth phase, leading to fewer clones in the pouch than the hinge.
pixL17 has increased cell death phenotype, enhanceable by RpS3[+]/RpS31
pixL17 has increased cell death phenotype, enhanceable by RpL14[+]/RpL141
pixL17 has decreased cell number | somatic clone phenotype, suppressible by Scer\GAL4en-e16E/BacA\p35UAS.cHa
BacA\p35UAS.cHa, Scer\GAL4en-e16E, pixL35/pixL17 has wing disc | posterior phenotype
The differences in pixL17 clone growth between the pouch and hinge are maintained, and even exaggerated, in a RpL141/+ background.
Although apoptosis is similar to wild-type controls in pixL17/+ wing discs, levels are enhanced in pixL17/+, RpL141/+ and pixL17/+, RpS31/+ double mutants.
pixL17, f36a clones induced in the wing disc 27 hours before larval wandering rarely survive to the adult wing. Those that do display a strong reduction in cell number but not cell size.
Expression of BacA\p35Scer\UAS.cHa in the posterior compartment of pixL35/pixL17 wing discs, driven by Scer\GAL4en-e16E, increases the area of the posterior compartment relative to control wings that also express BacA\p35Scer\UAS.cHa in the posterior. Expression of BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4en-e16E, in pixL17 wing disc clones increases the frequency of the clones but does not increase their size. The differences in pixL17 clone growth between the pouch and hinge are maintained, and even exaggerated, in a RpL141/+ background. Although apoptosis is similar to wild-type controls in pixL17/+ wing discs, levels are enhanced in pixL17/+, RpL141/+ and pixL17/+, RpS31/+ double mutants. pixL17, f36a clones induced in the wing disc 27 hours before larval wandering rarely survive to the adult wing. Those that do display a strong reduction in cell number but not cell size.
Expression of BacA\p35Scer\UAS.cHa in the posterior compartment of pixL35/pixL17 wing discs, driven by Scer\GAL4en-e16E, increases the area of the posterior compartment relative to control wings that also express BacA\p35Scer\UAS.cHa in the posterior.
Expression of BacA\p35Scer\UAS.cHa, under the control of Scer\GAL4en-e16E, in pixL17 wing disc clones increases the frequency of the clones but does not increase their size.