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General Information
Symbol
Dmel\pum02003
Species
D. melanogaster
Name
FlyBase ID
FBal0060928
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
pum2003
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The P{lacW} transposon in inserted between exons 8 and 9 on the pum transcription unit.

P{PZ} insertion in the intron between exons 8 and 9. The orientation of the P{PZ} element is such that the ry marker gene is towards the 5' side of the pum gene while the Ecol\lacZ gene is oriented towards the 3' side.

Insertion components
P{PZ}pum02003
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutants exhibit increased long term facilitation (LTF) at the neuromuscular junction.

Homozygous females do not lay eggs. Approximately 50% of homozygous larvae survive the pupal stage to eclosion. By the adult stage, 43% of homozygous females have germlineless ovaries, 32% have "differentiated ovaries" (containing recognisable egg chambers and/or germline cells in at least one ovariole) and 25% of females have one germlineless and one differentiated ovary. Homozygous female germline clones induced at the third larval instar stage produce ovarioles that contain on average 4.2 +/- 2.0 mature egg chambers but no other germ cells. Females containing homozygous female germline clones achieve an oviposition efficiency of 56% (for absolute number of eggs laid by the females). These eggs (derived from females containing homozygous germline clones mated to homozygous males) are sometimes capable of developing into adulthood.

Small ovary phenotype. None of the germ cells appear to undergo asymmetric divisions. Ovarioles contain only two clusters of apparently undifferentiated germline cells. Some ovaries in newly eclosed females completely lack germline cells though individual ovarioles are visible, in other ovaries the ovarioles are not recognisable. A small number of ovarioles contain 2-3 normally developing egg chambers that eventually develop into morphologically normal mature oocytes. Preoogenic germline development is also aberrant, germline stem cells of late third instar larval ovaries have no obvious spectrosome organisation.

Females are not able to maintain a normal germarial structure: instead of retaining active stem cells each ovariole houses only one or two germ line derivatives. When the non-functional egg chambers leave the germarium they are not replaced, the germarium assumes the appearance of a thin tube. Stem cells cannot be maintained and instead differentiate into cystoblast-like cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

pum02003 bamΔ86/pum9 bamΔ86 double mutant ovaries have a complex phenotype that is distinct from that of either single mutant. A mixture of apparently undifferentiated cells and overtly polyploid cells is seen. In many cases, the polyploid chromosomes are thick and expanded like nurse cell chromosomes. Occasionally these pseudo-nurse cells are organised within an epithelial layer of follicle cells, like a cyst, although these cysts never contain a full complement of 16 cystocytes. These cysts contain ring canals.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially complements
Partially rescued by

pum02003 is partially rescued by pumnos.PB

Comments

pum01688 partially complements pum02003 for oogenic defects and embryonic lethality.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Strong ovarette class mutation. P{PZ} mobilisation demonstrates the insertion is responsible for the mutant phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (11)