FlyBase Allele Report: Dmel\stj[k10814]

FB2026_02 , released June 18, 2026

FB2026_02 , released June 18, 2026

Allele: Dmel\stj^k10814

General Information

Symbol

Dmel\stj^k10814

Species

D. melanogaster

Name

FlyBase ID

FBal0064197

Feature type

allele

Associated gene

Associated Insertion(s)

P{lacW}stj^k10814

Carried in Construct

Key Links

Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Dickman et al., 2008)

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}stj^k10814

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Encoded product / tool

reporter enzyme

Tagged with

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:13,812,842..13,812,842 [-]

(Cunningham et al., 2022, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage (with Df(2R)CX1)

(Dickman et al., 2008)

abnormal locomotor behavior | adult stage (with Df(2R)Exel7128)

(Dickman et al., 2008)

abnormal locomotor behavior | adult stage | recessive

(Dickman et al., 2008)

abnormal neurophysiology | recessive | third instar larval stage

(Dickman et al., 2008)

abnormal neurophysiology | third instar larval stage (with Df(2R)CX1)

(Dickman et al., 2008)

flightless (with Df(2R)CX1)

(Dickman et al., 2008)

flightless (with Df(2R)Exel7128)

(Dickman et al., 2008)

flightless | recessive

(Dickman et al., 2008)

lethal (with stj^DD106)

(Dickman et al., 2008)

lethal (with stj^DD131)

(Dickman et al., 2008)

lethal (with stj^DD173)

(Dickman et al., 2008)

lethal (with stj^DD174)

(Dickman et al., 2008)

lethal (with stj^DD196)

(Dickman et al., 2008)

lethal | recessive

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

partially lethal - majority die (with Df(2R)CX1)

(Dickman et al., 2008)

partially lethal - majority die (with Df(2R)Exel7128)

(Dickman et al., 2008)

partially lethal - majority die | recessive

(Dickman et al., 2008)

some die during third instar larval stage (with Df(2R)CX1)

(Dickman et al., 2008)

some die during third instar larval stage (with Df(2R)Exel7128)

(Dickman et al., 2008)

some die during third instar larval stage | recessive

(Dickman et al., 2008)

Phenotype Manifest In

bouton & larval neuromuscular junction | supernumerary (with stj^DD106)

(Dickman et al., 2008)

larval somatic muscle cell (with stj^DD106)

(Dickman et al., 2008)

Detailed Description

Statement

Reference

stj^k10814/stj^k10814, stj^k10814/Df(2R)CX1 or stj^k10814/Df(2R)Exel7128 animals survives to adulthood if raised apart from heterozygous and wild-type siblings, but otherwise die during third instar. Adults are ataxic, flightless and die a few days after eclosion.

stj^k10814/stj^DD106 animals have an increase in branch number per-neuromuscular junction and a small (15%) but significant (p <0.05) increase in the mean number of boutons per neuro-muscular junction in third instar larvae despite decreased muscle size in these animals compared to controls. Normalized to muscle size, this corresponds to a 50% increase in bouton density. Electrophysiological recordings (0.3mM Ca[2+] 10mM Mg[2+] at neuromuscular junctions in these larvae or in stj^k10814/Df(2R)CX1 larvae show that the amplitude of miniature excitatory junction potentials (EJPs) is normal, but their frequency is significanly decreased. In contrast, the amplitude of evoked EJPs is dramatically reduced. This latter effect is seen across a range of Ca[2+] concentrations.

(Dickman et al., 2008)

External Data

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Df(2R)CX1/stj^k10814 has abnormal neurophysiology phenotype, suppressible | partially by cac^UAS.EGFP/Scer\GAL4^elav.PLu

(Dickman et al., 2008)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Df(2R)CX1/stj^k10814 has bouton & larval neuromuscular junction | supernumerary phenotype, non-suppressible by cac^UAS.EGFP/Scer\GAL4^elav.PLu

(Dickman et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

cac^{Scer\UAS.T:Avic\GFP-EGFP}; Scer\GAL4^elav.PLu fails to suppress the increase in bouton number or the reduction in evoked excitatory junction potentials (EJPs) seen at neuro-muscular junctions in stj^k10814/Df(2R)CX1 third instar larvae. However, the reduction in mini (spontaneous) EJPs seen at these junctions is suppressed.

(Dickman et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

stj^k10814/stj^DD106 is partially rescued by stj^UAS.Tag:HA/Scer\GAL4^elav.PLu

(Dickman et al., 2008)

Comments

stj^{Scer\UAS.T:Ivir\HA1}; Scer\GAL4^elav.PLu rescues the reduction of muscle size seen in stj^DD106/stj^k10814 third instar larvae and the increase in branch number per neuro-muscular junction but does not affect the increase in bouton number seen at neuromuscular junctions in these animals. It also rescues the reduction in evoked excitatory junction potentials seen at these junctions.

(Dickman et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (2)

11004

y¹ w^67c23; P{lacW}stj^k10814/CyO

102765

y¹ w^67c23 ; P{lacW}stj^k10814 / CyO

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Comments

Complements: fas⁰⁵⁴⁸⁸. Complements: l(2)k08708^k08708. Complements: l(2)03105^k16702.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

l(2)k10814

(Spradling et al., 1999)

l(2)k10814^k10814

stj^k10814

α2δ-3^k10814

(Zhang et al., 2023)

α2δ^k10814

(Cunningham et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections