FlyBase Allele Report: Dmel\CSN4[k08018]

General Information

Symbol

Dmel\CSN4^k08018

Species

D. melanogaster

Name

FlyBase ID

FBal0064351

Feature type

allele

Associated gene

Dmel\CSN4

Associated Insertion(s)

P{lacW}CSN4^k08018

Carried in Construct

Also Known As

l(2)K08018

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}CSN4^k08018

Cytology

Description

P{lacW} insertion 55bp downstream of the first ATG codon.

(Oron et al., 2002)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:7,972,675..7,972,675 [+]

(Doronkin et al., 2003, Oron et al., 2002, Suh et al., 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | adult stage | female | germline clone

(Pan et al., 2014)

lethal | recessive

(Oron et al., 2002, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotype Manifest In

female germline stem cell | somatic clone

(Pan et al., 2014)

Detailed Description

Statement

Reference

CSN4^k08018 mutant female germline stem cell (GSC) clones are lost from the niche much more quickly than wild type controls. No dramatic increase in apoptosis is seen in GSCs or cysts compared to controls. The remaining CSN4^k08018 mutant GSCs are able to differentiate correctly into germ cell cysts.

(Pan et al., 2014)

Oogenesis is normal in CSN4^k08018/+ flies.

(Doronkin et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

CSN4^k08018, CSN5^L4032 has increased cell death | oogenesis phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032 has increased occurrence of cell division | oogenesis phenotype

(Doronkin et al., 2003)

CSN4^k08018, Rpn6^k00103 has increased cell death | oogenesis phenotype

(Doronkin et al., 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

CSN4^k08018/CSN4[+] is a suppressor of cystoblast | increased number phenotype of bam^z3-2884/bam^Δ86

(Pan et al., 2014)

Other

Statement

Reference

CSN4^k08018, CSN5^L4032 has ovary phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032 has nurse cell phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032/CSN5[+] has egg chamber phenotype

(Doronkin et al., 2003)

CSN4^k08018, Rpn6[+]/Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN4^k08018, Rpn6[+]/Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

CSN4^k08018/CSN4[+], CSN5^L4032 has ovary phenotype

(Doronkin et al., 2003)

CSN4^k08018/CSN4[+], CSN5^L4032 has nurse cell phenotype

(Doronkin et al., 2003)

CSN4^k08018/CSN4[+], CSN5^L4032 has egg chamber phenotype

(Doronkin et al., 2003)

CSN4^k08018/CSN4[+], Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032 has egg chamber phenotype

(Doronkin et al., 2003)

CSN4^k08018/CSN4[+], Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

CSN4^k08018, Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN4^k08018, Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032/CSN5[+] has ovary phenotype

(Doronkin et al., 2003)

CSN4^k08018, CSN5^L4032/CSN5[+] has nurse cell phenotype

(Doronkin et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

One copy of CSN4^k08018 dramatically suppresses the increase in the number of undifferentiated cystoblasts seen in bam^z3-2884/bam^Δ86 mutants, dramatically increasing the number of differentiated cysts containing a branched fusome and the number of normal egg chambers per ovariole.

(Pan et al., 2014)

The ovaries of CSN5^L4032/CSN4^k08018 transheterozygotes exhibit apoptosis and extra cell division of nurse cells, as well as fused egg chambers. The penetrance of these phenotypes can exceed 50%. Unlike either single heterozygote, nurse cells in CSN4^k08018; Rpn6^k00103 transheterozygous females exhibit ectopic apoptosis, incorrect numbers of mitotic divisions and fusions of neighbouring egg chambers.

(Doronkin et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10765

y¹ w^67c23; P{lacW}CSN4^k08018/CyO

Kyoto

111236

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}CSN4^k08018 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Excision of the P{lacW} element can revert the lethal phenotype.

(Oron et al., 2002)

Complements: l(2)k08504^k08504.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

10765

(Oron et al., 2002)

CH4^k08018

CSN4^P

(Suh et al., 2002)

CSN4^k08018

(Betsch et al., 2019)

Csn4^k08018

(Zang and Bashaw, 2023, Pan et al., 2014)

Csn4^k

(Pan et al., 2014)

csn4^k08018

(Ullah et al., 2007)

l(2)K08018

(Oron et al., 2002)

l(2)k08018

(Spradling et al., 1999)

l(2)k08018^k08018

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

Open Close