FlyBase Allele Report: Dmel\RyR[k04913]

General Information

Symbol

Dmel\RyR^k04913

Species

D. melanogaster

Name

FlyBase ID

FBal0064520

Feature type

allele

Associated gene

Dmel\RyR

Associated Insertion(s)

P{lacW}RyR^k04913

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Gao et al., 2013)

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}RyR^k04913

Cytology

Description

P{lacW} insertion 399 bp upstream of the Rya-r44F start codon.

(Sullivan, 2001.4.25)

P{lacW} insertion in an intron, 255bp upstream of the first coding exon.

(Sullivan et al., 2000)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:8,862,556..8,862,556 [+]

(Sullivan, 2001.4.25, Sullivan et al., 2000, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of alcohol use disorder

CEA

(Adkins et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Feuillette et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant (with RyR^GS21220)

(Gao et al., 2013)

chemical resistant | semidominant

(Gao et al., 2013)

lethal | recessive

(Spradling et al., 1999)

Phenotype Manifest In

larval visceral muscle cell

(Sullivan et al., 2000)

Detailed Description

Statement

Reference

Heterozygotes and RyR^k04913/RyR^GS21220 animals show resistance to halothane compared to wild type.

RyR^k04913/RyR^GS21220 animals show resistance to sevoflurane, enflurane and isoflurane compared to wild type.

(Gao et al., 2013)

The body wall contraction (BWC) rate of heterozygous larvae on an agar substrate is nearly normal.

(Gao et al., 2004)

Rya-r44F^k04913 young larvae appear normal and die during first and second larval instar stages. A very mild, but reproducible defect in visceral muscle function, as assayed by ingestion, can be seen in mutants.

(Sullivan et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

RyR[+]/RyR^k04913 is an enhancer of visible | adult stage phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

NOT Enhancer of

Statement

Reference

RyR[+]/RyR^k04913 is a non-enhancer of partially lethal - majority die phenotype of Scer\GAL4^elav-C155, cac^{RQ,SL.UAS.EGFP}

(Brusich et al., 2018)

Suppressor of

Statement

Reference

Rya-r44F[+]/RyR^k04913 is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

NOT Suppressor of

Statement

Reference

RyR[+]/RyR^k04913 is a non-suppressor of partially lethal - majority die phenotype of Scer\GAL4^elav-C155, cac^{RQ,SL.UAS.EGFP}

(Brusich et al., 2018)

Other

Statement

Reference

Pkd2^ko67, RyR[+]/RyR^k04913 has abnormal locomotor behavior | larval stage phenotype

(Gao et al., 2004)

Phenotype Manifest In

Enhancer of

Statement

Reference

RyR[+]/RyR^k04913 is an enhancer of eye phenotype of Hsap\MAPT^UAS.cWa, Scer\GAL4^GMR.PF

(Feuillette et al., 2020)

Suppressor of

Statement

Reference

Rya-r44F[+]/RyR^k04913 is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

The body wall contraction (BWC) rate of Pkd2^ko67 RyR^k04913/Pkd2^ko67 + larvae on an agar substrate is reduced by 18% compared to wild-type larvae.

(Gao et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

RyR^GS21220/RyR^k04913 is rescued by RyR^UAS.Tag:V5/Scer\GAL4^nrv2.PS

(Gao et al., 2013)

Comments

Expression of RyR^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^nrv2.PS restores sensitivity to halothane in RyR^k04913/RyR^GS21220 animals.

(Gao et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10559

y¹ w^67c23; P{lacW}RyR^k04913/CyO

Kyoto

111172

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}RyR^k04913 /CyO y⁺

Notes on Origin

Discoverer

Separable from: a closely linked separable lethal mutation (the homozygous lethality of the chromosome is not rescued by RyR^{+tCH321-24D03}).

(Gao et al., 2013)

Comments

Phenotypic analysis suggest the allelic series: Df(2R)Np3 > Rya-r44F¹⁶ > Rya-r44F^k04913.

(Sullivan et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

RyR^K04913

(Terada et al., 2016)

RyR^k04913

(Feuillette et al., 2020, Adkins et al., 2017, Xu et al., 2017)

Rya-r44F^k04913

Ryr⁰⁴⁹¹³

(Gao et al., 2004)

dRyr^k04913

(Gao et al., 2013)

l(2)k04913

(Spradling et al., 1999)

l(2)k04913^k04913

(BDGP Project Members, 1994-1999)

rya-r44F^k04913

(Van Goethem et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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