FlyBase Allele Report: Dmel\pnt[S012309]

General Information

Symbol

Dmel\pnt^S012309

Species

D. melanogaster

Name

FlyBase ID

FBal0082328

Feature type

allele

Associated gene

Dmel\pnt

Associated Insertion(s)

P{lacW}pnt^S012309

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Delta2-3 transposase

(Deak et al., 1997)

P-element activity

(Salzberg et al., 1997)

Progenitor genotype

Associated Insertion(s)

P{lacW}pnt^S012309

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Salzberg et al., 1997)

Phenotype Manifest In

dorsal vessel wall cell | ectopic

(Alvarez et al., 2003)

embryonic pericardial cell

(Alvarez et al., 2003)

larval abdominal lch5 neuron | embryonic stage

(Salzberg et al., 1997)

Detailed Description

Statement

Reference

Mutant embryos have ectopic cardioblasts that form enlarged hearts comprising disorganised rows of cardioblasts. The ectopic cardioblasts appear to be restricted to the posterior domain. The numbers of pericardial cells is about half that of wild-type.

(Alvarez et al., 2003)

Lethality acts early and in the first larval instar.

(Deak et al., 1997)

Lethality occurs during the embryonic and larval stages. Dorsal localization of LCh5 in 1-2 segments per embryo.

(Salzberg et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

pnt^S012309 is a non-enhancer of dorsal vessel wall cell phenotype of pnr¹

(Alvarez et al., 2003)

pnt^S012309 is a non-enhancer of dorsal vessel wall cell phenotype of pnr^VX6

(Alvarez et al., 2003)

pnt^S012309 is a non-enhancer of embryonic pericardial cell phenotype of pnr^VX6

(Alvarez et al., 2003)

NOT Suppressor of

Statement

Reference

pnt^S012309 is a non-suppressor of dorsal vessel wall cell phenotype of pnr¹

(Alvarez et al., 2003)

pnt^S012309 is a non-suppressor of embryonic pericardial cell phenotype of pnr^VX6

(Alvarez et al., 2003)

pnt^S012309 is a non-suppressor of dorsal vessel wall cell phenotype of pnr^VX6

(Alvarez et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

pnr^VX6, pnt^S012309 double mutant embryos lack cardioblasts and pericardial cells and are phenotypically indistinguishable from pnr^VX6 embryos. In pnr¹, pnt^S012309 embryos local overproduction of cardioblasts are seen as seen in pnr¹ embryos.

(Alvarez et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

pnt^S012309 is rescued by Scer\GAL4^twi.PG/pnt^P2.UAS

(Alvarez et al., 2003)

Not rescued by

pnt^S012309 is not rescued by Scer\GAL4^twi.PG/pnt^P1.UAS

(Alvarez et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Analysis of excess cardioblast phenotype provides an allelic series:pnt^S012309, pnt² > pnt^RR112, pnt^rM254 > pnt^Δ88, pnt⁰⁷⁸²⁵.

(Alvarez et al., 2003)

Δ2-3 induced reversion demonstrates the insertion is responsible for the lethal phenotype.

(Salzberg et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

0123/09

(Deak et al., 1997)

123/9

(Salzberg et al., 1997)

l(3)012309

(Alvarez et al., 2003)

pnt^S012309

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close