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General Information
Symbol
Dmel\pntP2.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0035444
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-pntP2, UAS-pnt.P2, uas-pointedP2
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of the pnt P2 cDNA.

UASt regulatory sequences drive expression of the pnt P2 isoform.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The expression of pntP1.Scer\UAS under the control of Scer\GAL4prd.PU leads to no significant changes in the number of oenocytes in Prd-positive thoracic or abdominal segments during stage 15 of embryogenesis, as compared to controls.

Expression of pntP2.Scer\UAS under the control of either Scer\GAL4esg-NP5130 or Scer\GAL4Dl.PU (using tub-Gal80[ts] to limit the time of the transgene expression) results in intestinal stem cell overproliferation in the adult midgut.

Expression of pntP2.Scer\UAS under the control of Scer\GAL4hs.2sev results in 70% of ommatidia being symmetrical.

Overexpression of pntP2.Scer\UAS, under the control of Scer\GAL4en-e16E, induces vein loss in 0% in vein L4 and 89% in vein L5.

Expression of pntP2.Scer\UAS under the control of Scer\GAL4slbo.2.6 does not affect border cell motility.

Expression of pntP2.Scer\UAS driven by Scer\GAL4twi.PG has no effect on cardioblast development.

Expression of pntP2.Scer\UAS under the control of Scer\GAL4ey.PB does not result in homeotic transformation of the eye to antenna.

Expression of pntP2.Scer\UAS under the control of Scer\GAL4dpp.blk1 does not result in ectopic furrow formation in the eye disc.

Expression of pntP2.Scer\UAS under the control of Scer\GAL4nos.PG has no discernible effect on the viability or phenotype of embryos.

Scer\GAL4c532-mediated expression causes thinner and shorter dorsal appendages.

Scer\GAL455B and Scer\GAL4c324a mediated expression produces female progeny that lay wild type eggs that develop normally.

When expression is driven by Scer\GAL4hs.2sev, in an otherwise pntΔ88/pnt1277 background, an R7 photoreceptor is present in almost every ommatidium.

Ectopic expression of the P2 form of pnt driven by either Scer\GAL4rho.PL or Scer\GAL4sca-537.4 has no phenotypic effect.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
Suppressed by
NOT suppressed by
NOT Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

The increase in the adult midgut intestinal stem cell proliferation characteristic for flies expressing pntP2.Scer\UAS under the control of Scer\GAL4esg-NP5130 (using tub-Gal80[ts] to limit the time of the transgene expression) is not suppressed by co-expression of cicΔC2.Scer\UAS.T:Ivir\HA1.

Co-expression of kayScer\UAS.cEa has no effect on the symmetrical ommatidia phenotype caused by expression of pntP2.Scer\UAS under the control of Scer\GAL4hs.2sev.

Co-expression of either JraScer\UAS.cEa or aopEP598 partially suppresses the symmetrical ommatidia phenotype caused by expression of pntP2.Scer\UAS under the control of Scer\GAL4hs.2sev.

Co-expression of pntP2.Scer\UAS with mskScer\UAS.cLa, both under the control of Scer\GAL4en-e16E, further enhances the wing vein loss phenotype observed in pntP2.Scer\UAS single mutants, with vein loss to 97% in vein L4 and 100% in vein L5.

When phlScer\UAS.F179 and pntP2.Scer\UAS are coexpressed in the embryo under the control of Scer\GAL4en-e16E, the number of scolopidia in the lch5 organs is reduced from the normal number of 5 to 3 or 4 in 71% of hemisegments. When phlScer\UAS.F179 and pntP2.Scer\UAS are coexpressed under the control of Scer\GAL4GMR.PY in cells posterior to the morphogenetic furrow, most ommatidial clusters in the early stages of ommatidial assembly contain fewer than the normal number of neurons, with the specification of R3, R4, R1 and R6 being severely disrupted.

Xenogenetic Interactions
Statement
Reference

Co-expression of pntP2.Scer\UAS with Zzzz\aopPScer\UAS.T:Hsap\MYC under the control of Scer\GAL4GMR.PF does not alter the eye morphology of Zzzz\aopPScer\UAS.T:Hsap\MYC-expressing eyes.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
pntP2.Scer\UAS
pntP2.UAS
Name Synonyms
Secondary FlyBase IDs
  • FBal0035448
References (29)