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General Information
Symbol
Dmel\fzhs.sev
Species
D. melanogaster
Name
FlyBase ID
FBal0082914
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
sev-Fz
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

Two copies of the sev enhancer are placed upstream of an Hsp70 promoter driving fz expression.

sev expression vector (sev enhancer and inducible Hsp70 promoter) drives expression of fz.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of fzhs.sev results in more than 70% of ommatidia being symmetrical.

Expression of one copy of fzhs.sev causes strong planar cell polarity defects in the eye.

Flies expressing fzhs.sev show randomisation of chirality and rotation of the ommatidia. Many ommatidia are symmetrical, with both R4 and R3 cells developing as an R3 cell.

The ommatidia appear disorganised in flies expressing fzhs.sev, polarity is random and many ommatidial clusters remain symmetrical (showing R3/R3-type symmetry). In ommatidia that are mosaic in the R3/R4 photoreceptors, the fzhs.sev cell always adopts the R3 fate. This can lead to polarity reversals and inverted chirality.

Expression of fzhs.sev in a fz- background (fz15 or fz37) in either the presumptive R3 or R4 cell in mosaic ommatidia causes the cell expressing fzhs.sev to become an R3 cell and the other to become an R4 cell. Ommatidia with inappropriate chirality arise at the mosaic interface in eyes that are mosaic for fzhs.sev (in a wild-type background). In all these incorrectly patterned ommatidia, either one or both members of the R3/R4 pair carries fzhs.sev. In these cases, whenever the R3/R4 pair are mosaic, the R3 cell always carries fzhs.sev. The R3 cell also invariably carries fzhs.sev in chirally correct ommatidia that are mosaic for the R3/R4 pair.

Ommatidial orientations are disturbed, chiral shape is choosen randomly. Heat induced expression in embryos causes nearly all the cells secrete smooth cuticle and narrow denticle belts are observed (deletion of the first 3-4 anterior rows of denticles). Weakly affected denticle belts only affect the first row of denticles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

fzhs.sev has abnormal cell polarity phenotype, suppressible | partially by dcoj3B9/dco[+]

Phenotype Manifest In
Enhanced by
Statement
Reference

fzhs.sev has ommatidium phenotype, enhanceable by N[+]/N264-39

fzhs.sev has photoreceptor cell R4 phenotype, enhanceable by DlRevF10/Dl[+]

fzhs.sev has ommatidium phenotype, enhanceable by DlRevF10/Dl[+]

NOT Enhanced by
Statement
Reference

fzhs.sev has phenotype, non-enhanceable by exe1

fzhs.sev has phenotype, non-enhanceable by rinunspecified

Suppressed by
Statement
Reference

fzhs.sev has ommatidium phenotype, suppressible | partially by fw42

fzhs.sev has ommatidium phenotype, suppressible | partially by dcoj3B9/dco[+]

NOT suppressed by
Statement
Reference

fzhs.sev has phenotype, non-suppressible by exe1

fzhs.sev has phenotype, non-suppressible by rinunspecified

NOT Enhancer of
Statement
Reference

fzhs.sev is a non-enhancer of phenotype of exe1

NOT Suppressor of
Statement
Reference

fzhs.sev is a non-suppressor of phenotype of exe1

fzhs.sev is a non-suppressor of phenotype of dsh1

Additional Comments
Genetic Interactions
Statement
Reference

fw42/+ partially suppresses the symmetrical ommatidia phenotype caused by fzhs.sev. fw42/Y also partially suppresses the symmetrical ommatidia phenotype caused by fzhs.sev and in this case photoreceptor cell loss is also seen.

The planar polarity defects seen in the eyes of fzhs.sev/+ flies are significantly suppressed in fzhs.sev/+, dcoj3B9/+ flies, although the eyes do not appear wild type.

The fzhs.sev ommatidial phenotype is dominantly enhanced by N264-39 or DlRevF10; most fzhs.sev ommatidia show R3/R3-type symmetry in a N264-39 or DlRevF10 heterozygous background.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

The dominant fz phenotype in the eye caused by expression (in an otherwise wild-type background) of the fzhs.sev allele in the P{sev-fz.T} transgene (as reported in FBrf0099729) may be due to upregulation of fz transcription levels due to w enhancer sequences present in the P{sev-fz.T} transgene, since a construct (P{hs.2sev(-FRT)fz.1-1-1}) which also contains fz expressed under the control of sev enhancers and a heat shock promoter (fz1-1-1.hs.2sev), but which does not contain w sequences does not show a dominant fz phenotype in the eye when expressed in an otherwise wild-type background.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
fzhs.sev
Name Synonyms
Secondary FlyBase IDs
    References (15)