FlyBase Allele Report: Dmel\RhoGEF2[6.5]

General Information

Symbol

Dmel\RhoGEF2^6.5

Species

D. melanogaster

Name

FlyBase ID

FBal0085926

Feature type

allele

Associated gene

Dmel\RhoGEF2

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

antimorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

antimorphic allele - genetic evidence

(Nikolaidou, 2004)

Mutagen

ethyl methanesulfonate

(Barrett et al., 1997)

Progenitor genotype

Cytology

Description

Amino acid replacement: R1687C.

(Nikolaidou, 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:17,041,321..17,041,321 [+]

Nucleotide change:

C17041321T

Amino acid change:

Reported amino acid change:

R1687C

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Nikolaidou, 2004)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage (with RhoGEF2^4.1)

(Nikolaidou and Barrett, 2004)

lethal - all die during first instar larval stage (with RhoGEF2^4.1)

(Nikolaidou and Barrett, 2004)

Phenotype Manifest In

presumptive embryonic salivary gland (with RhoGEF2^4.1)

(Nikolaidou and Barrett, 2004)

Detailed Description

Statement

Reference

RhoGEF2^6.5/RhoGEF2^4.1 animals show 60% embryonic viability, 6% first larval instar viability and 0% adult viability. Salivary gland cells are not internalised in RhoGEF2^6.5/RhoGEF2^4.1 embryos, but remain on the surface (in contrast to wild-type embryos). Muscle and neuronal tissues show no significant defects in gross morphology in late-stage RhoGEF2^6.5/RhoGEF2^4.1 embryos.

(Nikolaidou and Barrett, 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

RhoGEF2^6.5 is a suppressor of visible phenotype of Rho1^GMR.PH

(Barrett et al., 1997)

Other

Statement

Reference

RhoGEF2^6.5, fog⁴/fog[+] has visible phenotype

(Nikolaidou and Barrett, 2004)

RhoGEF2^6.5/RhoGEF2[+], fog⁴ has visible | dominant phenotype

(Nikolaidou and Barrett, 2004)

Phenotype Manifest In

Suppressor of

Statement

Reference

RhoGEF2^6.5 is a suppressor of eye phenotype of Rho1^GMR.PH

(Barrett et al., 1997)

Other

Statement

Reference

RhoGEF2^6.5, fog⁴ has wing phenotype

(Nikolaidou and Barrett, 2004)

RhoGEF2^6.5/RhoGEF2[+], fog⁴ has wing phenotype

(Nikolaidou and Barrett, 2004)

Additional Comments

Genetic Interactions

Statement

Reference

16% of fog⁴/+ ; RhoGEF2^6.5/+ animals have wing defects.

(Nikolaidou and Barrett, 2004)

Dominantly suppresses the Rho1^GMR.PH rough eye phenotype.

(Barrett et al., 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9384

w^*; RhoGEF2^6.5/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

Notes on Origin

Discoverer

Comments

Isolated as a dominant suppressor of the Rho1^GMR.PH rough eye phenotype.

(Barrett et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

6.5

(Nikolaidou, 2004, Barrett et al., 1997)

DRhoGEF2^6.5

(Nikolaidou and Barrett, 2004)

RhoGEF2 6.5

(Levayer et al., 2011)

RhoGEF2^6.5

(Warrington et al., 2013)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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