FlyBase Allele Report: Dmel\bur[e10]

General Information

Symbol

Dmel\bur^e10

Species

D. melanogaster

Name

FlyBase ID

FBal0086521

Feature type

allele

Associated gene

Dmel\bur

Associated Insertion(s)

Carried in Construct

Also Known As

ES2-2^e10

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Long et al., 2006)

Mutagen

ethyl methanesulfonate

(Neufeld et al., 1998)

X ray

(Neufeld et al., 1998)

Progenitor genotype

Cytology

Description

Amino acid replacement: R61term.

(Long et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:21,177,419..21,177,419 [-]

Nucleotide change:

C21177419T

Reported nucleotide change:

C?T

Amino acid change:

R61term | bur-PB; R61term | bur-PC

Reported amino acid change:

R61term

(Long et al., 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Long et al., 2006)

lethal - all die before end of pupal stage | recessive

(Long et al., 2006)

some die during pupal stage | recessive

(Long et al., 2006)

Phenotype Manifest In

lamina

(Long et al., 2006)

medulla

(Long et al., 2006)

photoreceptor cell & axon

(Long et al., 2006)

photoreceptor cell R7 & growth cone

(Long et al., 2006)

photoreceptor cell R8 & growth cone

(Long et al., 2006)

Detailed Description

Statement

Reference

No defects in R-cell differentiation or in the organisation of the R-cell clusters is seen in homozygous eye imaginal discs.

Homozygous third instar larvae show severe defects in R-cell axon pathfinding; most, if not all R-cell axons are present in abnormally large bundles in the lamina and medulla, causing the appearance of hypo- and hyper-innervated regions. The terminal field is much smaller than wild type, the R7 and R8 growth cones fail to expand (most of them cannot be identified individually). The majority of R2-R5 axons terminate correctly within the lamina.

The differentiation and migration of the laminal glial cells appears largely normal in homozygous third instar larvae, although they appear less organised than normal and their density in the lamina is increased compared to wild type.

(Long et al., 2006)

Homozygous clones in the eye are wild-type.

(Neufeld et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

bur^e10 has abnormal neuroanatomy phenotype, non-suppressible by Mcm10^UAS.cLa/Scer\GAL4^GMR.PF

(Long et al., 2006)

Enhancer of

Statement

Reference

bur^e10/bur[+] is an enhancer of visible phenotype of Usp7^Δ35

(van der Knaap et al., 2010)

bur^e10/bur[+] is an enhancer of abnormal neuroanatomy phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Long et al., 2006)

bur^e10/bur[+] is an enhancer of abnormal neuroanatomy phenotype of Rac1^L89.UAS, Scer\GAL4^GMR.PF

(Long et al., 2006)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

bur^e10 has photoreceptor cell & axon phenotype, non-suppressible by Mcm10^UAS.cLa/Scer\GAL4^GMR.PF

(Long et al., 2006)

Enhancer of

Statement

Reference

bur^e10/bur[+] is an enhancer of wing phenotype of Usp7^Δ35

(van der Knaap et al., 2010)

bur^e10/bur[+] is an enhancer of lamina phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Long et al., 2006)

bur^e10/bur[+] is an enhancer of medulla phenotype of Rac1^N17.UAS, Scer\GAL4^GMR.PF

(Long et al., 2006)

bur^e10/bur[+] is an enhancer of photoreceptor cell & axon phenotype of Rac1^L89.UAS, Scer\GAL4^GMR.PF

(Long et al., 2006)

bur^e10 is an enhancer of phenotype of sina^GMR.PN

(Neufeld et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for bur^e10 enhances the "held-out wing" phenotype of Usp7^Δ35 hemizygotes.

(van der Knaap et al., 2010)

The R-cell axon projection defects caused by expression of Rac1^L89.Scer\UAS under the control of Scer\GAL4^GMR.PF are enhanced by bur^e10/+.

The axonal hyperfasciculation phenotype in the lamina and medulla caused by expression of Rac1^N17.Scer\UAS under the control of Scer\GAL4^GMR.PF is enhanced by bur^e10/+.

(Long et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

bur^e10 is partially rescued by bur^UAS.cLa/Scer\GAL4^elav-C155

(Long et al., 2006)

bur^e10 is partially rescued by bur^UAS.cLa/Scer\GAL4^GMR.PF

(Long et al., 2006)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: Dominant modifier of the sina^GMR.PN eye phenotype.

(Neufeld et al., 1998)

Comments

"ethyl methanesulfonate" was stated as tentative. "X ray" was stated as tentative.

(Neufeld et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

ES2-2^e10

(Long et al., 2006, Neufeld et al., 1998)

Gmps^e10

(van der Knaap et al., 2010)

bur^e10

(Long et al., 2006)

l(2)10523^e10

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close