FlyBase Allele Report: Dmel\CycB3[L6540]

General Information

Symbol

Dmel\CycB3^L6540

Species

D. melanogaster

Name

FlyBase ID

FBal0087021

Feature type

allele

Associated gene

Dmel\CycB3

Associated Insertion(s)

P{lacW}CycB3^L6540

Carried in Construct

Also Known As

l(3)L6540, CycB3¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}CycB3^L6540

Cytology

Description

P{lacW} inserted in 5'UTR of CycB3.

(Jacobs et al., 1998)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:24,870,794..24,870,794 [+]

(Spradling et al., 1999, Jacobs et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile | recessive

(Jacobs et al., 1998)

lethal | recessive

(Spradling et al., 1999)

viable

(Jacobs et al., 1998)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygotes are viable but developmentally delayed. Eclosion is delayed by 2 days.

(Jacobs et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

CycB3^L6540/CycB3[+] is an enhancer of visible | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

Suppressor of

Statement

Reference

CycB[+], CycB3^L6540, CycB², CycB3[+] is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PF, pbl^{ΔDH497-549.UAS}

(Gregory et al., 2007)

NOT Suppressor of

Statement

Reference

CycB3^L6540/CycB3[+] is a non-suppressor of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Other

Statement

Reference

CycA^C8LR1, CycB3^L6540, Df(2R)599-5 has lethal phenotype

(Jacobs et al., 1998)

CycA^C8LR1, CycB3^L6540 has lethal phenotype

(Jacobs et al., 1998)

CycB3^L6540, CycB² has lethal phenotype

(Jacobs et al., 1998)

CycB3^L6540, Df(2R)599-5 has lethal phenotype

(Jacobs et al., 1998)

Phenotype Manifest In

Enhancer of

Statement

Reference

CycB3^L6540/CycB3[+] is an enhancer of wing vein | ectopic | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

CycB3^L6540/CycB3[+] is an enhancer of wing | heat sensitive phenotype of H^C2.hs

(Johannes and Preiss, 2002)

Suppressor of

Statement

Reference

CycB[+], CycB3^L6540, CycB², CycB3[+] is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PF, pbl^{ΔDH497-549.UAS}

(Gregory et al., 2007)

NOT Suppressor of

Statement

Reference

CycB3^L6540/CycB3[+] is a non-suppressor of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

CycB3^L6540/CycB3[+] is a non-suppressor of ommatidium phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Other

Statement

Reference

CycA^C8LR1, CycB3^L6540, Df(2R)599-5 has embryo | embryonic cycle 15 phenotype

(Jacobs et al., 1998)

CycB3^L6540, Df(2R)599-5 has embryo | embryonic cycle 15 phenotype

(Jacobs et al., 1998)

CycA^C8LR1, CycB3^L6540 has spindle phenotype

(Jacobs et al., 1998)

CycA^C8LR1, CycB3^L6540 has embryo | embryonic cycle 15 phenotype

(Jacobs et al., 1998)

CycB3^L6540, CycB² has spindle phenotype

(Jacobs et al., 1998)

CycB3^L6540, CycB² has embryo | embryonic cycle 15 phenotype

(Jacobs et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

CycB3^L6540 CycA^C8LR1 double mutants show delayed chromatin condensation during prophase during mitosis 15 and rare mitotic cells with aberrant multipolar spindles at stage 13. CycB3^L6540, CycB^- (CycB² or Df(2R)599-5) double mutants have defective late mitotic figures during mitosis 15 and multipolar spindles at stage 13. CycB3^L6540 CycB^- (Df(2R)599-5) CycA^C8LR1 triple mutants are blocked at mitosis 15. 50% of embryos complete syncytial cycles without obvious defects, although many embryos were highly abnormal. Endoreduplicating cells still undergo DNA synthesis, though mitotic cells of the CNS do not.

(Jacobs et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10337

y¹ w¹¹¹⁸; P{lacW}CycB3^L6540/TM3, Ser¹

Kyoto

111053

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}82B P{lacW}CycB3^L6540/TM6B, P{Car20y}TPN1, Tb¹

Notes on Origin

Discoverer

Comments

FlyBase curator comment: Gregory et al., 2007, Fly 1(1): 13--22 state that "cycB3¹" equates to 'FBal0119683' but this is incorrect. 'FBal0119683' corresponds to "CycB3^unspecified", while "cycB3¹" is a synonym of "CycB3^L6540".

(Gregory et al., 2007)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CycB3¹

(Jacobs et al., 1998)

CycB3^L6540

(Garrido et al., 2020, Das et al., 2016, Tanaka-Matakatsu et al., 2007, Khurana et al., 2006)

CycB3^L6

(Garrido et al., 2020)

cycB3¹

(Gregory et al., 2007)

cycB3^L6540

(Chen et al., 2018)

l(3)L6540

(Spradling et al., 1999)

l(3)L6540^L6540

(Johannes and Preiss, 2002)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

Open Close