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General Information
Symbol
Dmel\mof2
Species
D. melanogaster
Name
FlyBase ID
FBal0088802
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    C5875642T

    Amino acid change:

    Q371term | mof-PA

    Comment:

    Site of nucleic acid change inferred from report that a truncated protein of 370 amino acids is made in the mutant.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Truncated protein of 370 instead of 827 amino acids.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Zygotic-only mof2 mutants (homozygotes) are male larval lethal and female semi-viable; female adults are short lived. Maternal-only mof2 mutants are lethal. Maternal-zygotic mof2 mutants are male embryonic lethal and female semi-lethal; all male embryos arrest development during or shortly after gastrulation with highly abnormal morphology such as irregular organization of epidermal tissues, aberrant invagination of the stomodaeum, and the hindgut, and failure to form a proper cephalic furrow.

    Epidermal mof2 clones in the mof+t6.8 background are able to form normal-looking adult structures with sensory bristles.

    mof2/Y hemizygous males are lethal

    Mutant males show full lethality, whereas the number of females reaching adulthood is reduced approximately 2-fold compared to wild type. Most mutant female adults are sterile. The average lifespan of mutant females is reduced to 8 days compared to 37 days in controls.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhancer of
    Statement
    Reference

    mof[+]/mof2 is an enhancer of partially lethal - majority die | dominant | female phenotype of scsisB-1, sisA[+]/sisA1

    NOT Enhancer of
    Statement
    Reference
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    One paternally-derived copy of mof2 reduces the viability of scsisB-1 sisA1/+ females.

    Xenogenetic Interactions
    Statement
    Reference

    One copy of mof2 does not enhance the reduction in eclosion rate seen when Hsap\HTTQ93.ex1p.Scer\UAS is expressed under the control of Scer\GAL4elav-C155.

    Complementation and Rescue Data
    Comments

    The lethality of mof2 hemizygous mutant males is strongly rescued by expression of any of the following:mofScer\UAS.T:Zzzz\FLAG, mof2KN.Scer\UAS.T:Zzzz\FLAG, mof7KN.Scer\UAS.T:Zzzz\FLAG, mof9KN.Scer\UAS.T:Zzzz\FLAG, mof3KC.Scer\UAS.T:Zzzz\FLAG or mof9KC.Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4arm.PU and partially also by expression of mof9KC.Scer\UAS.T:Zzzz\FLAG, whereas expression of either mofΔN.Scer\UAS.T:Zzzz\FLAG or mofNt.Scer\UAS.T:Zzzz\FLAG does not efficiently rescue survival of the male mutants as only few escapers emerge.

    Expression of mofScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4arm.PU completely rescues the lethality of mof2 males.

    Expression of either mofΔZn.Scer\UAS.T:Ivir\HA1, mofW426G.Scer\UAS.T:Ivir\HA1 or mofY416D.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4arm.PU fails to rescue the lethality of mof2 males.

    The lethality of mof2 males is partially rescued by expression of either mofΔ1.Scer\UAS.T:Ivir\HA1 (49.6% rescue), mofΔ2.Scer\UAS.T:Ivir\HA1 (59.3% rescue), mofΔ3.Scer\UAS.T:Ivir\HA1 (42.5% rescue) or mofΔN.Scer\UAS.T:Ivir\HA1 (11.2% rescue) under the control of Scer\GAL4arm.PU.

    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (1)
    References (12)