UAS regulatory sequences drive expression of a mutated form of mof containing the amino acid replacement Y416D within the Chromo domain. The protein is tagged at the N-terminal end with Tag:HA.
mofY416D.UAS.Tag:HA/Scer\GAL4arm.PU fails to rescue mof2
Expression of mofY416D.Scer\UAS.T:Ivir\HA1 under the control of Scer\GAL4arm.PU fails to rescue the lethality of mof2 males.