FlyBase Allele Report: Dmel\htl[UAS.cMa]

General Information

Symbol

Dmel\htl^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Michelson

FlyBase ID

FBal0090412

Feature type

allele

Associated gene

Dmel\htl

Associated Insertion(s)

Carried in Construct

P{UAS-htl.M}

Also Known As

UAS-Htl, UAS-htl^WT

Key Links

Transgenic product class

wild_type

(Michelson et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Michelson et al., 1998)

Mutagen

in vitro construct

(Michelson et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-htl.M}

Cytology

Description

Full length htl is expressed under the control of UAS regulatory sequences.

(Michelson et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

htl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates childhood spinal muscular atrophy

modeled by Smn^{RNAi.C.UAS.WIZ}

(Sen et al., 2011)

modeled by Smn^{RNAi.N.UAS.WIZ}

(Sen et al., 2011)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | third instar larval stage, with Scer\GAL4^how-24B

(Sen et al., 2011)

Phenotype Manifest In

axon & mechanosensory neuron & adult head, with Scer\GAL4^Bx-MS1096, Scer\GAL4^Mz1277, Scer\GAL4^twi.PB, htl^YY262/htl^AB42

(Garcia-Alonso et al., 2000)

axon & ocellus sensory structure, with Scer\GAL4^Bx-MS1096, Scer\GAL4^Mz1277, Scer\GAL4^twi.PB, htl^YY262/htl^AB42

(Garcia-Alonso et al., 2000)

embryonic/larval neuromuscular junction | third instar larval stage, with Scer\GAL4^how-24B

(Sen et al., 2011)

myoblast | increased number, with Scer\GAL4¹¹⁵¹

(Maqbool et al., 2006)

tarsal levator muscle cell, with Scer\GAL4¹¹⁵¹

(Maqbool et al., 2006)

visceral muscle fiber | increased number, with Scer\GAL4¹¹⁵¹

(Maqbool et al., 2006)

Detailed Description

Statement

Reference

Expression of htl^Scer\UAS.cMa driven by Scer\GAL4^how-24B does not significantly affect the number of boutons per muscle at the neuromuscular junction (NMJ) of third instar larvae, compared to controls.

Evoked excitatory junction potential (EJP) and miniature EJP (mEJP) amplitude are significantly decreased (there is no significant difference in quantal content or mEJP frequency) at the NMJ of Scer\GAL4^how-24B>htl^Scer\UAS.cMa third instar larvae, compared to controls.

(Sen et al., 2011)

Expression of htl^Scer\UAS.cMa in developing myoblasts under the control of Scer\GAL4¹¹⁵¹ results in an increase in the number of myoblasts (specifically tilm myoblasts). Consistent with these larval phenotypes, over-expression leads to a significantly higher number of fibres contributing to leg muscles. For example, the average number of htl^Scer\UAS.cMa-induced muscle fibres (counted in 10 adult legs) of the tarsus levator muscle (talm) is 14.8 compared to 10 fibres in the wild-type. However, the overall leg muscle pattern and capacity of fibres to interact with internal tendons appears unchanged.

(Maqbool et al., 2006)

Expression of htl^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1 has no effect on furrow formation in the eye disc.

(Kumar and Moses, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

htl^UAS.cMa/Scer\GAL4^how-24B is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^how-24B, Smn^{RNAi.C.UAS.WIZ}

(Sen et al., 2011)

htl^UAS.cMa/Scer\GAL4^how-24B is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^how-24B, Smn^{RNAi.N.UAS.WIZ}

(Sen et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

htl^UAS.cMa/Scer\GAL4^how-24B is a suppressor of NMJ bouton | third instar larval stage phenotype of Scer\GAL4^how-24B, Smn^{RNAi.C.UAS.WIZ}

(Sen et al., 2011)

htl^UAS.cMa/Scer\GAL4^how-24B is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Scer\GAL4^how-24B, Smn^{RNAi.C.UAS.WIZ}

(Sen et al., 2011)

htl^UAS.cMa/Scer\GAL4^how-24B is a suppressor of NMJ bouton | third instar larval stage phenotype of Scer\GAL4^how-24B, Smn^{RNAi.N.UAS.WIZ}

(Sen et al., 2011)

htl^UAS.cMa/Scer\GAL4^how-24B is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Scer\GAL4^how-24B, Smn^{RNAi.N.UAS.WIZ}

(Sen et al., 2011)

htl^UAS.cMa/Scer\GAL4^MS1075 is a suppressor of axon & adult head | conditional ts phenotype of Nrg^l10

(Garcia-Alonso et al., 2000)

NOT Suppressor of

Statement

Reference

htl^UAS.cMa/Scer\GAL4^twi.PB is a non-suppressor of mesoderm phenotype of pbl³

(Schumacher et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of htl^Scer\UAS.cMa rescues the phenotype of reduced bouton number per muscle at the neuromuscular junction of Scer\GAL4^how-24B>Smn^{dsRNA.N.Scer\UAS.WIZ} or Scer\GAL4^how-24B>Smn^{dsRNA.C.Scer\UAS.WIZ} third instar larvae.

(Sen et al., 2011)

Mesoderm spreading and differentiation in pbl³ homozygous embryos is not rescued by htl^Scer\UAS.cMa; Scer\GAL4^twi.PB.

(Schumacher et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

htl^UAS.cMa/Scer\GAL4^twi.PG rescues htl^AB42

(Wilson et al., 2005)

htl^UAS.cMa/Scer\GAL4^twi.PB rescues htl^AB42

(Schumacher et al., 2004)

htl^UAS.cMa/Scer\GAL4^twi.PG rescues htl^AB42

(Bradley et al., 2003)

htl^UAS.cMa/Scer\GAL4^twi.PG rescues htl^AB42

(Michelson et al., 1998)

Partially rescues

htl^UAS.cMa/Scer\GAL4^alrm.PD partially rescues htl^AB42

(Stork et al., 2014)

htl^UAS.cMa/Scer\GAL4^htl.POS partially rescues htl^AB42

(Rudolf et al., 2013)

htl^UAS.cMa/Scer\GAL4^tey-5053A partially rescues htl^AB42

(Reim et al., 2012)

htl^UAS.cMa, Scer\GAL4^twi.PB, Scer\GAL4^Mz1277, Scer\GAL4^Bx-MS1096 partially rescues htl^YY262/htl^AB42

(Garcia-Alonso et al., 2000)

Fails to rescue

htl^UAS.cMa/Scer\GAL4^twi.PB fails to rescue htl^YY262/htl^AB42

(Garcia-Alonso et al., 2000)

Comments

Expression of htl^Scer\UAS.cMa under the control of Scer\GAL4^alrm.PD strongly restores infiltration of astrocyte processes into the neuropil in htl^AB42 embryos, however, migration of the ventral-most astrocyte is not rescued.

(Stork et al., 2014)

Expression of htl^Scer\UAS.cMa under the control of Scer\GAL4^htl.POS restores dorsal muscle development in approximately half of htl^AB42 embryos. Some of the rescued embryos develop cardiac and pericardial cells.

(Rudolf et al., 2013)

Expression of htl^Scer\UAS.cMa under the control of Scer\GAL4^tey-5053A rescues the longitudinal visceral muscle (LVM) founder cell death seen in htl^AB42 mutant stage 13 embryos. The founder cell migration defects are also partially rescued, however some cells acquire abnormal shapes, adhere to positions more distant from the TVM both dorsally and ventrally, and form clusters, resulting in missing LVM cells in the most anterior trunk segments.

(Reim et al., 2012)

Expression of htl^Scer\UAS.cMa under the control of Scer\GAL4^twi.PG is able to induce bilaterally symmetrical flattening of the mesodermal tube onto the ectoderm in the early stages of mesoderm morphogenesis in htl^AB42 embryos.

(Wilson et al., 2005)

htl^Scer\UAS.cMa; Scer\GAL4^twi.PB rescues the migration of the mesoderm after invagination in htl^AB42 embryos. As a result the number of hemisegments with eve expressing mesodermal cells is 22, just as in wild-type.

(Schumacher et al., 2004)

Expression of htl^Scer\UAS.cMa under the control of Scer\GAL4^twi.PG rescues the formation of the visceral mesoderm and salivary gland migration in htl^AB42/htl^AB42 embryos.

(Bradley et al., 2003)

The addition of htl^Scer\UAS.cMa driven by Scer\GAL4^twi.PB fails to rescue the lethality seen in htl^YY262/htl^AB42. However, when htl^Scer\UAS.cMa is driven by Scer\GAL4^Mz1277, Scer\GAL4^Bx-MS1096 and Scer\GAL4^twi.PB concurrently, some escaper pupae do survive.