FB2021_01, released Feb 18, 2021

Allele: Ecol\lacZUAS.Tag:NLS(SV40-largeT)

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General Information
Symbol
Ecol\lacZUAS.Tag:NLS(SV40-largeT)
Species
E. coli
Name
FlyBase ID
FBal0092785
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{UAS-lacZ.NZ}
Also Known As
UAS-nlacZ, UAS-nlsLacZ, UAS-lacZ.NZ, UAS-lacZ, UAS-nls-lacZ, UAS-lacZnls, UAS-lacZnls, UAS-NZ, UAS-nuclear lacZ
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
in vitro construct - regulatory fusion
(Bloomington Drosophila Stock Center, 1998.9.14)
in vitro construct - coding region fusion
(Bloomington Drosophila Stock Center, 1998.9.14)
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
       
      Progenitor genotype
      Carried in construct
      P{UAS-lacZ.NZ}
      Cytology
      Nature of the lesion
      Statement
      Reference

      A lacZ reporter tagged with a nuclear localization signal (Tag:NLS(SV40-largeT)) is expressed under the control of UAS regulatory sequences.

      (Bloomington Drosophila Stock Center, 1998.9.14)
      Allele components
      Product class / Tool use(s)
      Regulatory region(s)
      UAS
      Encoded product / tool
      lacZ
      Tagged with
      Tag:NLS(SV40-largeT)
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference

      Scer\GAL4-targeted reporter: expression of Ecol\lacZ reflects action of enhancer/promoter elements driving GAL4 in the same cell.Product localized to nucleus: the β-galactosidase protein produced carries a nuclear localization signal.

      (Huang and Fischer-Vize, 1996)

      Scer\GAL4-targeted reporter; β-galactosidase distribution reflects action of enhancer/promoter elements driving GAL4 in the same cell.Product localized to nucleus: carries T:SV40\nls2 nuclear localization signal.

      (Huang and Fischer-Vize, 1996)
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Retina expressing Ecol\lacZScer\UAS.T:SV40\nls2 with prosZzzz\CAG.127Q.Scer\UAS.T:Ivir\HA1, under the control of Scer\GAL4GMR.PF does not suppress the toxic effects of prosZzzz\CAG.127Q.Scer\UAS.T:Ivir\HA1 expression.

      (Kazemi-Esfarjani and Benzer, 2002)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (8)
      Notes on Origin
      Comments
      Comments

      Expresses nuclear Ecol\lacZ.

      (Huang and Fischer-Vize, 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (8)
      Reported As
      Symbol Synonym
      Ecol\lacZScer\UAS.T:SV40\nls-largeT
      Ecol\lacZScer\UAS.T:SV40\nls2
      Ecol\lacZUAS.Tag:NLS(SV40-largeT)
      lacZT:nls2
      lacZUAS.T:nls2
      lacZUAS.nuc
      lacZnuc.UAS
      nuclearLacZ
      (Garrity et al., 1999)
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0056740
      References (70)