FlyBase Recombinant Construct Report: P{UAS-lacZ.NZ}

General Information

Symbol

P{UAS-lacZ.NZ}

FlyBase ID

FBtp0001611

Feature type

transgenic_transposable_element

Component Allele(s)

Ecol\lacZ^{UAS.Tag:NLS(SV40-largeT)}

Also Known As

UAS-nlacZ, UAS-lacZ, UAS-nlsLacZ, UAS-lacZ.NZ, UAS-nls-lacZ, UAS-lacZnls, UAS-lacZ^nls, UAS-NZ, UAS-nuclear lacZ, UAS-nucLacZ

Size

Expression Data

lacZ reporter

Associated insertion(s)

5 available

Molecular map

Description and Uses

Construct components

Component

Use(s)

Marker(s)

w^+mC

Component allele Ecol\lacZ^{UAS.Tag:NLS(SV40-largeT)}

Transgenic product class

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(SV40-largeT)

nuclear protein localization tag

Description

A lacZ reporter tagged with a nuclear localization signal (Tag:NLS(SV40-largeT)) is expressed under the control of UAS regulatory sequences.

(Bloomington Drosophila Stock Center, 1998.9.14)

Sequence Data

Sequence (FB)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA Sequence

Extent

Segments and Size

Total Size

Left end

Right end

Segments

Num

Orientation

Symbol

Reference

Phenotypic Data

Component Allele

Phenotypic Data

Ecol\lacZ^{UAS.Tag:NLS(SV40-largeT)}

Phenotypic Class Phenotype Manifest In Detailed Description

Expression Data

Reporter Expression

Additional Information

Statement

Reference

ScerGAL4-targeted reporter: expression of EcollacZ reflects action of enhancer/promoter elements driving GAL4 in the same cell.Product localized to nucleus: the β-galactosidase protein produced carries a nuclear localization signal.

(Huang and Fischer-Vize, 1996)

ScerGAL4-targeted reporter; β-galactosidase distribution reflects action of enhancer/promoter elements driving GAL4 in the same cell.Product localized to nucleus: carries @ T:SV40

ls2@ nuclear localization signal.

(Huang and Fischer-Vize, 1996)

Marker for

Reflects expression of

Reporter construct used in assay

Progenitors and Descendants

progenitor(s)

pP{UAST}

(Bloomington Drosophila Stock Center, 1998.9.14)

descendant(s)

Comments

report comment: Produces β-galactosidase containing the SV40\nls2 nuclear localization signal.

(Bloomington Drosophila Stock Center, 1998.9.14)

mapping_comment: β-gal localized to the nucleus.

(Bloomington Drosophila Stock Center, 1998.9.14)

construct_comment: Progenitors are those of the plasmid pP{UAS-lacZ.NZ}.Yash Hiromi and S. West, unpublished.

(Bloomington Drosophila Stock Center, 1998.9.14)

Stocks (8)

Stocks containing insertion(s) of this recombinant construct

Bloomington

3956

w¹¹¹⁸; P{UAS-lacZ.NZ}J312

4410

y¹ w^*; P{AyGAL4}25 P{UAS-lacZ.NZ}20b/CyO, y⁺

51943

y¹ w¹¹¹⁸; βTub60D^Pin-1/CyO; P{nSyb-GAL4.P}attP2, P{UAS-lacZ.NZ}J312/TM6B, Tb¹

51946

y¹ w¹¹¹⁸; βTub60D^Pin-1/CyO; P{nSyb-GAL4.DBD::QF.AD}attP2, P{UAS-lacZ.NZ}J312/TM6B, Tb¹

99587

P{hsFLP}12, P{tubP-GAL80}LL1 w^* P{neoFRT}19A; P{UAS-lacZ.NZ}20b, P{UAS-mCD8::GFP.L}LL5; P{tubP-GAL4}LL7/TM6B, Tb⁺

Kyoto

107531

w¹¹¹⁸; P{UAS-lacZ.NZ}20b

107532

w¹¹¹⁸; P{UAS-lacZ.NZ}J312

107723

y¹ w^*; P{AyGAL4}25 P{UAS-lacZ.NZ}20b/CyO, y⁺

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (37)

Reported As

Symbol Synonym

5XUAS-LacZ

(Meraner et al., 2026)

NLS-β-Gal

(Shi and Skeath, 2004)

P{UAS-NZ}

(Bloomington Drosophila Stock Center, 1998.9.14)

P{UAS-lacZ.NZ}

(Li et al., 2011, Kim et al., 2006, FlyBase, 1996-)

P{w^+mC=UAS-lacZ.NZ}

(Li and Lu, 2009)

UAS nuclear lacZ

(Venkatesh and Shyamala, 2010)

UAS-LZ

(Huang et al., 2013)

UAS-LacZ

(Suyama et al., 2023, Herrera et al., 2021, Kremer et al., 2010, Buch et al., 2008)

UAS-LacZ.NZ

(Rideout et al., 2010, Billeter and Goodwin, 2004, Hatini and DiNardo, 2001)

UAS-NLS-lacZ

(Yamada et al., 2003)

UAS-NZ

(Lee and Park, 2004, Suzuki and Saigo, 2000)

UAS-NlacZ

(Yoshii et al., 2008, Kido and Ito, 2002)

UAS-lacZ

(Huang et al., 2013, Marchal et al., 2012, Sato et al., 1999)

UAS-lacZ-nls

(Sens et al., 2010)

UAS-lacZ.NZ

(Park et al., 2011, Guerin and Kramer, 2009, Ward et al., 2002)

UAS-lacZ:nls

(Mikeladze-Dvali et al., 2005)

UAS-lacZ^nls

(Li et al., 2011, Dominguez et al., 2004)

UAS-lacZNZ

(Sens et al., 2010)

UAS-lacZnls

(Siekhaus et al., 2010, Bruckner et al., 2004, Bruckner et al., 2004, Sen et al., 2004)

UAS-nLacZ

(Tu et al., 2021, Tayler et al., 2012)

UAS-nLacz

(Awasaki et al., 2006)

UAS-nlacZ

(Baliga et al., 2016, Huang et al., 2013, Cho and Fischer, 2011, Palanker et al., 2009, Baker et al., 2007, Mondal et al., 2007, Palanker et al., 2006, Astle et al., 2003, Baker et al., 2003, Bello et al., 2003, Kozlova and Thummel, 2003, Kozlova and Thummel, 2002, Badenhorst, 2001)

UAS-nlacZ^7.4

(Huang et al., 2013)

UAS-nls-lacZ

(Monier et al., 2005, Rival et al., 2004, Rival et al., 2004, Schwaerzel et al., 2002)

UAS-nls::LacZ

(Besson et al., 2011)

UAS-nlsLacZ

(Wang et al., 2006, Cenci and Gould, 2005)

UAS-nlslacZ

(Pantazis et al., 2008, Bello et al., 2007, Brodu et al., 2004, Brodu et al., 2002)

UAS-nlslacZ^J312

(Bello et al., 2006)

UAS-nucLacZ

(Diao et al., 2024, Rozich et al., 2023)

UAS-nuclear lacZ

(Makhijani et al., 2007, Jhaveri et al., 2000)

UAS.lacZ

(Lawrence et al., 2004)

UAS.lacZ^J312

(Lawrence et al., 2002)

UASnuclacZ

(Rangarajan et al., 1999)

UASp:lacZ

(Jermusyk et al., 2016)

nuclear Β

(Kazemi-Esfarjani and Benzer, 2002)

uas-LacZ^nls

(Shafer and Taghert, 2009)

uas-lacZ^nls

(Shafer and Taghert, 2009)

Secondary FlyBase IDs

References (77)

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