FlyBase Allele Report: Dmel\asl[2]

General Information

Symbol

Dmel\asl²

Species

D. melanogaster

Name

FlyBase ID

FBal0093161

Feature type

allele

Associated gene

Dmel\asl

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Bonaccorsi et al., 1998)

Progenitor genotype

Cytology

Description

No lesion detected in the asl transcription unit.

(Varmark et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | larval stage

(Giansanti et al., 2001)

abnormal mitotic cell cycle | larval stage (with asl³)

(Giansanti et al., 2001)

lethal (with asl³)

(Bonaccorsi et al., 2000, Bonaccorsi et al., 1998)

lethal | recessive

(Bonaccorsi et al., 2000, Bonaccorsi et al., 1998)

viable (with asl¹)

(Bonaccorsi et al., 1998)

Phenotype Manifest In

anaphase & condensed nuclear chromosome

(Giansanti et al., 2001)

anaphase & condensed nuclear chromosome (with asl³)

(Giansanti et al., 2001)

aster

(Bonaccorsi et al., 2007)

centriole (with Df(3R)ED5177)

(Galletta et al., 2016)

centrosome

(Giansanti et al., 2001)

centrosome (with asl³)

(Giansanti et al., 2001)

ciliary rootlet (with asl³)

(Chen et al., 2015)

germline stem cell (with Df(3R)ED5177)

(Galletta et al., 2016)

larval brain & ganglion mother cell

(Bonaccorsi et al., 2000)

larval brain & ganglion mother cell (with asl³)

(Bonaccorsi et al., 2000)

larval brain & ganglion mother cell & aster

(Bonaccorsi et al., 2000)

larval brain & ganglion mother cell & aster (with asl³)

(Bonaccorsi et al., 2000)

larval brain & ganglion mother cell & centrosome

(Bonaccorsi et al., 2000)

larval brain & ganglion mother cell & centrosome (with asl³)

(Bonaccorsi et al., 2000)

larval brain & neuroblast

(Bonaccorsi et al., 2000)

larval brain & neuroblast (with asl³)

(Bonaccorsi et al., 2000)

larval brain & neuroblast & aster

(Wakefield et al., 2001, Bonaccorsi et al., 2000)

larval brain & neuroblast & aster (with asl³)

(Bonaccorsi et al., 2000)

larval brain & neuroblast & centrosome

(Wakefield et al., 2001, Bonaccorsi et al., 2000)

larval brain & neuroblast & centrosome (with asl³)

(Bonaccorsi et al., 2000)

microtubule

(Giansanti et al., 2001)

microtubule (with asl³)

(Giansanti et al., 2001)

microtubule basal body & spermatocyte (with Df(3R)ED5177)

(Blachon et al., 2008)

mitosis & nuclear chromosome

(Giansanti et al., 2001)

mitosis & nuclear chromosome (with asl³)

(Giansanti et al., 2001)

neuroblast & nucleus

(Giansanti et al., 2001)

neuroblast & nucleus (with asl³)

(Giansanti et al., 2001)

spermatocyte & aster

(Bonaccorsi et al., 1998)

spermatocyte & aster (with asl¹)

(Bonaccorsi et al., 1998)

spermatocyte & aster (with asl³)

(Bonaccorsi et al., 1998)

Detailed Description

Statement

Reference

asl²/Df(3R)ED5177 male third instar larvae also display abnormally long centrioles in their germline stem cells compared to controls.

(Galletta et al., 2016)

Ciliary rootlets fail to form in the olfactory neurons of asl²/asl³ adults.

(Chen et al., 2015)

asl²/Df(3R)ED5177 spermatocytes completely lack basal bodies.

(Blachon et al., 2008)

The anastral mitotic spindles have a microtubule density similar to that of wild type in asl² larval brains. The spindles of mutant ganglion mother cells are morphologically indistinguishable from wild type.

The average length of ganglion mother cell and neuroblast spindles in homozygous larval brains is similar to that of wild type.

(Bonaccorsi et al., 2007)

Mutant neuroblasts show robust chromatin-mediated microtubule nucleation and spindle assembly producing fairly normal spindles during mitosis. Spindles poles emerge from a disorganised microtubule array near the chromosomes that focusses as the spindle lengthens. Spindles do not rotate during formation, always forming along the initial pole separation axis, but do rotate during metaphase. Consecutive divisions in the mutants usually produce adjacent or near-adjacent daughters, as in wild type. In a few cases, spindles form parallel to the ganglion mother cell cap, these neuroblasts divide symmetrically.

(Rusan and Peifer, 2007)

The mitotic index and frequencies of metaphase and anaphase figures in homozygous and asl²/asl³ larval brains is comparable to wild type. 4-5% of anaphases have lagging chromosomes. A small fraction of metaphases appear to be polyploid; 5-6% hyperploid and 2.6-2.8% tetraploid metaphases are seen. Homozygous and asl²/asl³ larval neuroblasts lack functional centrosomes and are completely devoid of astral microtubules. However, they form central spindles that are indistinguishable from wild type. The central spindle is tightly associated with the nascent ganglion mother cell nucleus, which is in turn closely apposed to the polar cortex, as occurs in wild-type cells. However, wild-type and mutant telophases differ in the positioning of the neuroblast (NB) nucleus; in wild-type, the NB nucleus lies very close to the central spindle but is separated from the polar cortex by a large astral array of microtubules, however the situation is reversed in mutant telophases; the NB nucleus is usually disconnected from the central spindle and located much closer to the polar cortex than in wild type.

(Giansanti et al., 2001)

Neuroblasts in mutant larval brains lack functional centrosomes and astral microtubules, but have well-focused spindle poles. In homozygous testes, telophases show a morphologically normal central spindle and cytokinesis occurs normally.

(Wakefield et al., 2001)

Lethality acts at the larval/pupal boundary. Prophase neuroblasts are completely devoid of centrosomes and asters, however a bipolar spindle does form and generates a normal central spindle in anaphase. Chromosome segregation and cytokinesis appear to be normal. Mitosis in GMCs reveals an absence of centrosomes and prophase asters.

(Bonaccorsi et al., 2000)

Homozygotes and asl²/asl³ larvae die at the larval/pupal boundary. asl²/asl¹ animals are viable. Homozygous, asl²/asl¹ and asl²/asl³ spermatocytes are completely devoid of asters.

(Bonaccorsi et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhancer of

Statement

Reference

asl² is an enhancer of meiotic telophase II & spindle phenotype of asp¹

(Wakefield et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The microtubule density of mitotic spindles in lkb1³¹⁵ asl² double mutant larval brains is similar to that seen in lkb1³¹⁵ single mutants.

(Bonaccorsi et al., 2007)

The microtubule minus ends of asl² asp¹ double mutant larval brain neuroblasts are splayed at metaphase and fail to converge into focused spindle poles.

(Wakefield et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

asl³

(Bonaccorsi et al., 2000)

asl¹

(Bonaccorsi et al., 1998)

Comments

Fails to complement asl¹ for male and female sterility and aberrant spermatid phenotype.

(Bonaccorsi et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

asl²

(Matsuura et al., 2024, Zheng et al., 2020, Galletta et al., 2016, Chen et al., 2015, Kitazawa et al., 2012, Blachon et al., 2008, Bonaccorsi et al., 2007, Rusan and Peifer, 2007, Varmark et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (14)

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