FlyBase Allele Report: Dmel\ap[UAS.cMa]

General Information

Symbol

Dmel\ap^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Milan

FlyBase ID

FBal0093166

Feature type

allele

Associated gene

Dmel\ap

Associated Insertion(s)

Carried in Construct

P{UAS-ap.M}

Also Known As

UAS-Ap

Key Links

Transgenic product class

cDNA

(Milan et al., 1998)

wild_type

(Milan et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Milan et al., 1998)

wild_type

(Milan et al., 1998)

Mutagen

in vitro construct

(Milan et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-ap.M}

Cytology

Description

A full length ap cDNA (B8) is expressed under the control of UASt regulatory sequences.

(Milan et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^dpp.blk1

(Milan and Cohen, 1999)

Phenotype Manifest In

dorsocentral bristle, with Scer\GAL4^pnr-MD237

(Ramain et al., 2000)

wing blade ventral compartment, with Scer\GAL4^dpp.blk1

(Milan and Cohen, 1999)

wing disc | somatic clone, with Scer\GAL4^Act5C.PP

(Milan and Cohen, 2003)

wing margin | ectopic, with Scer\GAL4^dpp.blk1

(Milan and Cohen, 1999)

Detailed Description

Statement

Reference

When ap^Scer\UAS.cMa expressing clones (driven by Scer\GAL4^Act5C.PP) are made in the ventral part of the wing disc, they are significantly rounder than wild-type clone. ap^Scer\UAS.cMa, Scer\GAL4^Act5C.PP clones also fail to respect the dorsal ventral boundary.

(Milan and Cohen, 2003)

ap^Scer\UAS.cMa; Scer\GAL4^Act5C.PP somatic clones are recovered much less frequently in the ventral compartment of 3rd instar wing discs than in the dorsal compartment. The few surviving ventral clones adopt a more rounded shape than dorsal clones or wild-type control clones.

(Milan et al., 2002)

Expression of ap^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544 does not affect wing development.

(Weihe et al., 2001)

Expression of ap^Scer\UAS.cMa when driven by Scer\GAL4^pnr-MD237 leads to a loss of dorsocentral bristles.

(Ramain et al., 2000)

Flies expressing ap^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1 produce ectopic wing margins on both sides of the stripe of Scer\GAL4^dpp.blk1 expression in the ventral compartment of the wing.

(Milan and Cohen, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^ap-md544/ap^UAS.cMa is a suppressor of visible phenotype of Bx¹

(Bejarano et al., 2008, Milan et al., 2004)

ap^UAS.cMa/Scer\GAL4^vg.boundary is a suppressor | partially of visible phenotype of vg^null/vg^83b27

(Delanoue et al., 2002)

ap^UAS.cMa, Scer\GAL4^ap-md544 is a suppressor of visible phenotype of Chi^UAS.cMa, Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

Scer\GAL4^ap-md544/ap^UAS.cMa is a suppressor of visible | dominant phenotype of Bx¹

(Milan et al., 1998)

NOT Suppressor of

Statement

Reference

ap^UAS.cMa, Scer\GAL4^ap-md544 is a non-suppressor of visible phenotype of Chi^ΔLID.UAS, Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^ap-md544/ap^UAS.cMa is a suppressor of wing margin phenotype of Bx¹

(Bejarano et al., 2008, Milan et al., 2004)

ap^UAS.cMa/Scer\GAL4^vg.boundary is a suppressor | partially of wing phenotype of vg^null/vg^83b27

(Delanoue et al., 2002)

ap^UAS.cMa, Scer\GAL4^ap-md544 is a suppressor of wing phenotype of Chi^UAS.cMa, Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

Scer\GAL4^ap-md544/ap^UAS.cMa is a suppressor of wing phenotype of Bx¹

(Milan et al., 1998)

NOT Suppressor of

Statement

Reference

ap^UAS.cMa, Scer\GAL4^ap-md544 is a non-suppressor of wing disc phenotype of Bx^UAS.Tag:HA, Scer\GAL4^ap-md544

(Weihe et al., 2001)

ap^UAS.cMa, Scer\GAL4^ap-md544 is a non-suppressor of alar lobe phenotype of Chi^ΔLID.UAS, Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

ap^UAS.cMa, Scer\GAL4^ap-md544 is a non-suppressor of wing | distal phenotype of Chi^ΔLID.UAS, Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

ap^UAS.cMa, Scer\GAL4^ap-md544 is a non-suppressor of wing margin phenotype of Bx^EP1306, Scer\GAL4^ap-md544

(Milan and Cohen, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The Bx¹ scalloped wing phenotype is completely suppressed by expression of ap^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544.

(Milan et al., 2004)

The loss of ventrally located somatic clones of ap^Scer\UAS.cMa; Scer\GAL4^Act5C.PP cells in late third instar wing discs is suppressed by BacA\p35^Scer\UAS.cHa, mam^EPgJ3-285, or N^{ECN.Scer\UAS.cGa}, but not by trn^Scer\UAS.cMa, caps^Scer\UAS.cSa, caps^ID.Scer\UAS, or trn^{ΔC.Scer\UAS.T:Hsap\MYC}.

(Milan et al., 2002)

Co-expression of ap^Scer\UAS.cMa does not rescue wing pouch growth in wing discs expressing Bx^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^ap-md544.

(Weihe et al., 2001)

Suppresses the wing phenotype caused by expression of Chi^Scer\UAS.cMa under the control of Scer\GAL4^ap-md544. Does not suppress the wing phenotype caused by expression of Chi^{ΔLID.Scer\UAS} under the control of Scer\GAL4^ap-md544. Does not suppress the wing phenotype caused by expression of Bx^EP1306 under the control of Scer\GAL4^ap-md544.

(Milan and Cohen, 1999)

The Bx¹ wing phenotype is completely suppressed by ap^Scer\UAS.cMa expressed under the control of Scer\GAL4^ap-md544.

(Milan et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^ap-md544/ap^UAS.cMa rescues ap^md544

(Milan and Cohen, 2003)

Scer\GAL4^ap-md544/ap^UAS.cMa rescues ap^md544/ap^P44

(Milan and Cohen, 1999)

Comments

Rescues the wing defects of ap^md544/ap^P44 flies when expressed under the control of Scer\GAL4^ap-md544.

(Milan and Cohen, 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ap^Scer\UAS.cMa

ap^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Milan

(Milan et al., 1998)

Secondary FlyBase IDs

References (13)

Report Sections

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