A 736bp otu promoter fragment drives expression of a cDNA encoding the 104kD otu isoform. A 0.4kb fragment containing the otu polyadenylation site is fused downstream of the cDNA.
Almost 90% of gonads are oogenic in newly eclosed XX tra1/tra4 pseudomales that are also carrying otu104.H. The level of feminisation is markedly reduced 7-10 days after eclosion. The feminisation of XX tra1/tra4 gonads by otu104.H is blocked if they are also carrying Df(1)otu-PΔ1.