XX tra¹/tra⁴ pseudomales that are also homozygous for ovo^D1rv23 show an increase in the frequency of atrophic gonads compared to XX tra¹/tra⁴ pseudomales. Non-oogenic gonads, with spectrosomes and multibranched fusomes are also seen (at the same frequency as XX tra¹/tra⁴ pseudomales). XX tra¹/tra⁴ pseudomales that are also homozygous for Df(1)otu-PΔ1 show an increase in the frequency of atrophic gonads and a decrease in the frequency of oogenic gonads compared to XX tra¹/tra⁴ pseudomales. Non-oogenic gonads, with spectrosomes and multibranched fusomes are seen at the same frequency as XX tra¹/tra⁴ pseudomales. XX tra¹/tra⁴ pseudomales that are also homozygous for Sxl^f5 show a decrease in the frequency of oogenic gonads and an increase in the frequency of non-oogenic gonads compared to XX tra¹/tra⁴ pseudomales. Atrophic gonads are seen at the same frequency as XX tra¹/tra⁴ pseudomales. XX tra¹/tra⁴ pseudomales that are also carrying otu^hs.PN and raised at 20-25^oC are 2 to 3 times longer than normal. Expression of otu^hs.PN partially rescues XX tra¹/tra⁴ pseudomales; 88% of the gonads are 'feminised' and show a developmental progression of gametogenic stages, which can be divided into three sections corresponding to germarial regions 1-3 in normal oogenesis. Section I includes the most apical portion of the pseudomale testis and contains gametogenic stages characteristic of germarial region 1 in ovaries. A group of cells containing spectrosomes is localised at the distal tip. Clusters of cells connected by branched fusomes are located immediately adjacent to the distal cells. The fusomes have disappeared in section II and differentiation of the ring canals continues. In section II, the pseudomale germ cells have differentiated to postgermarial stages. The germ cell clusters contain thick ring canals and the nuclei are large. Oogenic gonads are not seen in XX tra¹/tra⁴ pseudomales carrying otu^hs.PN that are also homozygous for ovo^D1rv23 or Sxl^f5. In each case the frequency of non-oogenic gonads is increased compared to XX tra¹/tra⁴ pseudomales also homozygous for ovo^D1rv23 or Sxl^f5 respectively. Almost 90% of gonads are oogenic in newly eclosed XX tra¹/tra⁴ pseudomales that are also carrying otu^104.H. The level of feminisation is markedly reduced 7-10 days after eclosion. The feminisation of XX tra¹/tra⁴ gonads by otu^104.H is blocked if they are also carrying Df(1)otu-PΔ1.

Expression of Zzzz\tra^fl.Scer\UAS under the control of Scer\GAL4^arm.PS in XX pseudomales homozygous for tra⁴ reduces the number of bristles forming the male sex comb structure in the foreleg basitarsus. In some cases, intersexual bristles are found between the sex comb-like bristles. In a very few cases, in which no sex comb-like bristles are found, the last transverse row is usually only partially rotated. Both male and female external terminalia are found. These comprise the female 8th tergite and vaginal plate structures, plus the male genital arch, lateral plates, claspers and penis apparatus. The anal plates are always intersexual. Increasing the dose of Zzzz\tra^fl.Scer\UAS leads to a reduction in the frequency of male structures, and a corresponding increase in the frequency of female structures - although complete female transformation is never observed.