FlyBase Allele Report: Dmel\eIF4A[1006]

General Information

Symbol

Dmel\eIF4A¹⁰⁰⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0095427

Feature type

allele

Associated gene

Dmel\eIF4A

Associated Insertion(s)

P{lacW}eIF4A¹⁰⁰⁶

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gao et al., 2000, Galloni and Edgar, 1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}eIF4A¹⁰⁰⁶

Cytology

Description

Insertion into the second intron of eIF-4a.

(Galloni and Edgar, 1999)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:5,983,237..5,984,010

(Wang et al., 2025, Coelho et al., 2005, Gao et al., 2000, Galloni and Edgar, 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

amnioserosa

(Li et al., 2005)

amnioserosa (with Df(2L)Gpdh1-A)

(Li et al., 2005)

cyst progenitor cell | decreased number | somatic clone

(Wang et al., 2025)

eye

(Galloni and Edgar, 1999)

female germline stem cell | germline clone

(Shen et al., 2009)

wing disc

(Galloni and Edgar, 1999)

Detailed Description

Statement

Reference

Less than 9% of eIF-4a¹⁰⁰⁶ germline stem cells detected 1 week after clone induction (ACI) are detected at 3 weeks ACI, whereas 76% of control germline stem cells detected 1 week ACI are detected at 3 weeks ACI.

eIF-4a¹⁰⁰⁶ germline stem cells divide slower than wild type germline stem cells, resulting in the prodcution of fewer cysts.

(Shen et al., 2009)

eIF-4a¹⁰⁰⁶ clones in the wing disc show no regional differences in their ability to grow during both the fast and slow growth phases.

(Coelho et al., 2005)

Homozygous and eIF-4a¹⁰⁰⁶/Df(2L)GpdhA embryos have an increased number of amnioserosa cells compared to wild type.

(Li et al., 2005)

Mutants are deficient in the exit from cellular quiescence and have a DNA replication block.

(Galloni and Edgar, 1999)

Larval growth arrest, in L1. 60% survive until 6 days after hatching. 10% survive until 8 days after hatching. DNA replication ceases. Mutant phenotype is cell autonomous. Mutant clones in the wing disc are virtually undetectable. All adults in which clones had been induced show a rough eye phenotype. Mutant clones in the eye produce a rough eye phenotype, almost no mutant cells are detected.

(Galloni and Edgar, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

eIF-4a[+]/eIF4A¹⁰⁰⁶ is a suppressor of female sterile phenotype of bam^z3-2884/bam^Δ86

(Shen et al., 2009)

eIF4A¹⁰⁰⁶ is a suppressor of increased cell number | somatic clone phenotype of Pten^dj189

(Gao et al., 2000)

Phenotype Manifest In

Suppressor of

Statement

Reference

eIF-4a[+]/eIF4A¹⁰⁰⁶ is a suppressor of ovariole phenotype of bam^z3-2884/bam^Δ86

(Shen et al., 2009)

eIF-4a[+]/eIF4A¹⁰⁰⁶ is a suppressor of germarium phenotype of bam^z3-2884/bam^Δ86

(Shen et al., 2009)

eIF-4a[+]/eIF4A¹⁰⁰⁶ is a suppressor of female germline stem cell | increased number phenotype of bam^z3-2884/bam^Δ86

(Shen et al., 2009)

NOT Suppressor of

Statement

Reference

eIF-4a[+]/eIF4A¹⁰⁰⁶ is a non-suppressor of germarium phenotype of bam^Δ86

(Shen et al., 2009)

eIF-4a[+]/eIF4A¹⁰⁰⁶ is a non-suppressor of ovariole phenotype of bam^Δ86

(Shen et al., 2009)

Other

Statement

Reference

Pten^dj189, eIF4A¹⁰⁰⁶ has eye disc | somatic clone phenotype

(Gao et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Most bam^z3-2884/bam^Δ86; eIF-4a¹⁰⁰⁶/+ ovarioles contain 4-6 normal egg chambers, while the germaria contain fewer single germ cells compared to bam^z3-2884/bam^Δ86 ovarioles.

eIF-4a¹⁰⁰⁶ fails to dominantly suppress the tumourous phenotype of bam^Δ86 ovarioles.

(Shen et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

eIF4A^YE9

(Li et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Excision of the P-element is accompanied by reversion of the mutant phenotype.

(Galloni and Edgar, 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

eIF-4a¹⁰⁰⁶

eIF4A¹⁰⁰⁶

(Wang et al., 2025, Rode et al., 2018, Shen et al., 2009, Li et al., 2005)

eIF4a¹⁰⁰⁶

(Insco et al., 2009)

eIf4A¹⁰⁰⁶

(Galloni and Edgar, 1999)

eif4A¹⁰⁰⁶

(Galloni and Edgar, 1999)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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