FlyBase Allele Report: Dmel\kek1[UAS.cGa]

General Information

Symbol

Dmel\kek1^UAS.cGa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Ghiglione

FlyBase ID

FBal0096870

Feature type

allele

Associated gene

Dmel\kek1

Associated Insertion(s)

Carried in Construct

P{UAS-kek1.G}

Also Known As

UAS-kek1

Key Links

Transgenic product class

wild_type

(Ghiglione et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Ghiglione et al., 1999)

Mutagen

in vitro construct

(Ghiglione et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-kek1.G}

Cytology

Description

The complete kek1 coding region is expressed under the control of UASt regulatory sequences.

(Ghiglione et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

kek1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^71B

(Wessells et al., 1999)

visible, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004, Bai et al., 2001)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Duchek and Rorth, 2001)

dorsal appendage, with Scer\GAL4^T155

(Ghiglione et al., 1999)

egg, with Scer\GAL4^T155

(Ghiglione et al., 1999)

egg chorion, with Scer\GAL4^CY2

(Alvarado et al., 2004)

egg chorion, with Scer\GAL4^T155

(Ghiglione et al., 1999)

eye, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004, Ghiglione et al., 2003, Bai et al., 2001)

ommatidium, with Scer\GAL4^GMR.PF

(Alvarado et al., 2004)

photoreceptor neuron, with Scer\GAL4^GMR.PF

(Ghiglione et al., 2003)

posterior crossvein, with Scer\GAL4^en-e16E

(Ghiglione et al., 2003)

wing, with Scer\GAL4^71B

(Wessells et al., 1999)

wing vein, with Scer\GAL4^71B

(Wessells et al., 1999)

wing vein, with Scer\GAL4^Bx-MS1096

(Ghiglione et al., 2003)

wing vein L4, with Scer\GAL4^en-e16E

(Ghiglione et al., 2003)

wing vein L5, with Scer\GAL4^en-e16E

(Ghiglione et al., 2003)

Detailed Description

Statement

Reference

Expression of kek1^Scer\UAS.cGa under the control of Scer\GAL4^CY2 results in eggs with strongly ventralised chorions. Expression of kek1^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PF results in a rough eye phenotype.

(Alvarado et al., 2004)

kek1^Scer\UAS.cGa; Scer\GAL4^Bx-MS1096 flies have a general vein loss phenotype. In kek1^Scer\UAS.cGa; Scer\GAL4^en-e16E flies this vein loss phenotype is restricted to the posterior compartment of the wing. kek1^Scer\UAS.cGa; Scer\GAL4^GMR.PF flies have substantially reduced numbers of photoreceptors and eye size.

(Ghiglione et al., 2003)

When expression is driven by Scer\GAL4^GMR.PF the eyes are rough and small.

(Bai et al., 2001)

Dorsal migration of border cells is severely inhibited when kek1^Scer\UAS.cGa is expressed under the control of either Scer\GAL4^slbo.2.6, with only minor effects on the initial posterior migration. Most eggs derived from these females do not hatch.

(Duchek and Rorth, 2001)

Eggs derived from females expressing kek1^Scer\UAS.cGa under the control of Scer\GAL4^T155 are longer than wild-type. They show a reduction or complete loss of dorsal appendages, due to a ventralisation of the eggshell.

(Ghiglione et al., 1999)

Overexpression of kek1^Scer\UAS.cGa driven by Scer\GAL4^71B results in loss of veins in the adult wing.

(Wessells et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, kek1^UAS.cGa has visible phenotype, enhanceable by ed^UAS.cBa/Scer\GAL4^GMR.PF

(Bai et al., 2001)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, kek1^UAS.cGa has visible phenotype, suppressible by Egfr[+]/Egfr^SOK2

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has visible phenotype, suppressible by Egfr[+]/Egfr^SOK3

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has visible phenotype, suppressible by Egfr[+]/Egfr^SOK4

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has visible phenotype, suppressible | partially by Egfr[+]/Egfr^SOK5

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has visible phenotype, suppressible by Egfr[+]/Egfr^SOK1

(Alvarado et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, kek1^UAS.cGa has eye phenotype, enhanceable by ed^UAS.cBa/Scer\GAL4^GMR.PF

(Bai et al., 2001)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, kek1^UAS.cGa has eye phenotype, suppressible | partially by Egfr[+]/Egfr^SOK5

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has ommatidium phenotype, suppressible by Egfr[+]/Egfr^SOK1

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has ommatidium phenotype, suppressible by Egfr[+]/Egfr^SOK2

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has ommatidium phenotype, suppressible by Egfr[+]/Egfr^SOK3

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has ommatidium phenotype, suppressible by Egfr[+]/Egfr^SOK4

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has ommatidium phenotype, suppressible | partially by Egfr[+]/Egfr^SOK5

(Alvarado et al., 2004)

Scer\GAL4^CY2, kek1^UAS.cGa has egg chorion phenotype, suppressible by Egfr[+]/Egfr^SOK3, Scer\GAL4^CY2

(Alvarado et al., 2004)

Scer\GAL4^CY2, kek1^UAS.cGa has egg chorion phenotype, suppressible by Egfr[+]/Egfr^SOK4, Scer\GAL4^CY2

(Alvarado et al., 2004)

Scer\GAL4^CY2, kek1^UAS.cGa has egg chorion phenotype, suppressible | partially by Egfr[+]/Egfr^SOK5

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has eye phenotype, suppressible by Egfr[+]/Egfr^SOK1

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has eye phenotype, suppressible by Egfr[+]/Egfr^SOK2

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has eye phenotype, suppressible by Egfr[+]/Egfr^SOK3

(Alvarado et al., 2004)

Scer\GAL4^GMR.PF, kek1^UAS.cGa has eye phenotype, suppressible by Egfr[+]/Egfr^SOK4

(Alvarado et al., 2004)

Suppressor of

Statement

Reference

kek1^UAS.cGa/Scer\GAL4^CY2 is a suppressor of egg chorion phenotype of Scer\GAL4^CY2, grk^ΔTC.UAS

(Alvarado et al., 2004)

kek1^UAS.cGa, Scer\GAL4^CY2 is a suppressor of egg chorion phenotype of Egfr::kek1^{KEΔCG.UAS.GFP}, Scer\GAL4^CY2

(Alvarado et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of kek1^Scer\UAS.cGa in the follicle cells completely suppresses the dorsalised chorion phenotype caused by expression of grk^{ΔTC.Scer\UAS} under the control of Scer\GAL4^CY2. The dorsalised chorion phenotype caused by expression of Egfr::kek1^{KEΔCG.Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^CY2 is completely suppressed by coexpression of kek1^Scer\UAS.cGa. The rough eye phenotype caused by expression of kek1^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PF is suppressed if they are heterozygous for Egfr^SOK1/+, Egfr^SOK2/+, Egfr^SOK3/+, Egfr^SOK4/+ or Egfr^SOK5/+. The ventralised chorion phenotype caused by expression of kek1^Scer\UAS.cGa under the control of Scer\GAL4^CY2 is suppressed by Egfr^SOK3/+ or Egfr^SOK4/+ and to a lesser extent by Egfr^SOK5/+.

(Alvarado et al., 2004)

The ventralised phenotype of eggs derived from females expressing kek1^Scer\UAS.cGa under the control of Scer\GAL4^T155 is not altered if the females are also coexpressing rho^Scer\UAS.cGa. The ventralised phenotype of eggs derived from females expressing kek1^Scer\UAS.cGa under the control of Scer\GAL4^T155 is overridden if the females are also coexpressing btl::Egfr^{Scer\UAS.T:λ\cI-DD} or phl^{Scer\UAS.F179}; the resulting eggs are dorsalised.

(Ghiglione et al., 1999)

The addition of vn^Scer\UAS.cSa to flies expressing kek1^Scer\UAS.cGa driven by Scer\GAL4^71B results in an additive phenotype, with both vein loss and ectopic vein material seen.

(Wessells et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

kek1^Scer\UAS.cGa

kek1^UAS.cGa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Ghiglione

(Ghiglione et al., 1999)

Secondary FlyBase IDs

References (6)

Report Sections

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