FlyBase Allele Report: Dmel\grk[ΔTC.UAS]

General Information

Symbol

Dmel\grk^ΔTC.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0103804

Feature type

allele

Associated gene

Dmel\grk

Associated Insertion(s)

Carried in Construct

P{UAS-grk.ΔTC}

Also Known As

UAS-sgrk, UAS-s-grk, UAS-grkΔTC

Key Links

Transgenic product class

missense_variant

(Queenan et al., 1999)

stop_gained

(Queenan et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Queenan et al., 1999)

stop_gained

(Queenan et al., 1999)

Mutagen

in vitro construct

(Queenan et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-grk.ΔTC}

Cytology

Description

A Stop codon is inserted 11 amino acids after the EGF motif. The last amino acid before the stop is changed from aspartate to glycine. The truncated cDNA is driven by a UASt sequences.

(Queenan et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

grk

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^en-e16E

(Queenan et al., 1999)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Poukkula et al., 2011, McDonald et al., 2006, Duchek and Rorth, 2001)

dorsal appendage, with Scer\GAL4^CY2

(Queenan et al., 1999)

egg chorion, with Scer\GAL4^CY2

(Alvarado et al., 2004)

wing, with Scer\GAL4^en-e16E

(Queenan et al., 1999)

wing vein | ectopic, with Scer\GAL4^CY2

(Queenan et al., 1999)

wing vein | ectopic, with Scer\GAL4^en-e16E

(Queenan et al., 1999)

wing vein L4, with Scer\GAL4^en-e16E

(Queenan et al., 1999)

Detailed Description

Statement

Reference

Induction of grk^{ΔTC.Scer\UAS} by Scer\GAL4^esg-NP7397 does not affect the proliferation of adult midgut progenitor cells.

(Jiang and Edgar, 2009)

Border cells migrate normally when grk^{ΔTC.Scer\UAS} is expressed under the control of Scer\GAL4^cb41 in follicle cell clones.

Expression of one copy of grk^{ΔTC.Scer\UAS} in the border cell cluster, driven by Scer\GAL4^slbo.2.6, causes a defects in border cell migration in egg chambers with around a 30% penetrance. Increasing the number of copies of the grk^{ΔTC.Scer\UAS} transgene increases the penetrance and severity of the phenotype.

(McDonald et al., 2006)

Expression of grk^{ΔTC.Scer\UAS} under the control of Scer\GAL4^CY2 results in eggs with severely dorsalised chorions.

(Alvarado et al., 2004)

100% of border cell clusters migrate normally to the oocyte in egg chambers expressing grk^{ΔTC.Scer\UAS} under the control of Scer\GAL4^Act5C.PI in anterior follicle cell clones.

(McDonald et al., 2003)

Border cell migration is affected when grk^{ΔTC.Scer\UAS} is expressed under the control of either Scer\GAL4^slbo.2.6.

(Duchek and Rorth, 2001)

Expression of grk^{ΔTC.Scer\UAS} under the control of Scer\GAL4^ey.PB does not result in homeotic transformation of the eye to antenna.

(Kumar and Moses, 2001)

When expressed in ovarian follicle cells under the control of Scer\GAL4^CY2, eggs laid become dorsalised. When expressed in the wing disc under the control of Scer\GAL4^CY2, extra wing veins are produced. When expressed under the control of Scer\GAL4^en-e16E, very few flies survive, however in escapers, an expansion of wing vein 4 up to the compartment boundary is seen in both wings, though this expansion does not extend over the boundary.

(Queenan et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^hh.PU/grk^ΔTC.UAS is a suppressor of increased cell death | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

Scer\GAL4^vn-GAL4/grk^ΔTC.UAS is a suppressor | heat sensitive | partially of lethal - all die before end of pupal stage phenotype of vn^L6/vn^GAL4

(Austin et al., 2014)

Other

Statement

Reference

Scer\GAL4^vn-GAL4, grk^ΔTC.UAS, vn^L6/vn^GAL4 has lethal - all die before end of pupal stage phenotype

(Austin et al., 2014)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^slbo.2.6, grk^ΔTC.UAS has border follicle cell phenotype, suppressible by Pvr^UAS.cDa, Scer\GAL4^slbo.2.6

(Poukkula et al., 2011)

Scer\GAL4^CY2, grk^ΔTC.UAS has egg chorion phenotype, suppressible by kek1^UAS.cGa/Scer\GAL4^CY2

(Alvarado et al., 2004)

Suppressor of

Statement

Reference

Scer\GAL4^hh.PU/grk^ΔTC.UAS is a suppressor of wing disc posterior compartment | larval stage phenotype of Scer\GAL4^hh.PU, Spyo\Cas9^UAS.P2, wg^{UAS.wg+intergenic-1.pCFD6}

(Ewen-Campen and Perrimon, 2024)

Scer\GAL4^vn-GAL4/grk^ΔTC.UAS is a suppressor of leg phenotype of vn^L6/vn^GAL4

(Austin et al., 2014)

Other

Statement

Reference

Scer\GAL4^vn-GAL4, grk^ΔTC.UAS, vn^L6/vn^GAL4 has wing vein | ectopic phenotype

(Austin et al., 2014)

Scer\GAL4^vn-GAL4, grk^ΔTC.UAS, vn^L6/vn^GAL4 has wing vein phenotype

(Austin et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of grk^{ΔTC.Scer\UAS} under the control of Scer\GAL4^vn.GAL4 rescues the lethality of vn^L6/vn^GAL4 mutants to the pharate adult stage. The wings have fused L3 and L4 veins and extra vein material. The wing disc phenotype is partially rescues and no leg defects are observed. The extent of the rescue depends on the level of expression, but viable flies are never seen. Very high levels of expression are toxic, with animals dying as pupae.

(Austin et al., 2014)

Co-expression of kek1^Scer\UAS.cGa in the follicle cells completely suppresses the dorsalised chorion phenotype caused by expression of grk^{ΔTC.Scer\UAS} under the control of Scer\GAL4^CY2.

(Alvarado et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

grk^{ΔTC.Scer\UAS}

grk^ΔTC.UAS

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

Open Close