FlyBase Allele Report: Dmel\kn[+m5]

General Information

Symbol

Dmel\kn^+m5

Species

D. melanogaster

Name

FlyBase ID

FBal0097028

Feature type

allele

Associated gene

Dmel\kn

Associated Insertion(s)

Carried in Construct

P{col5-cDNA.C}

Also Known As

P[col5-cDNA]

Key Links

Transgenic product class

wild_type

(Crozatier and Vincent, 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Crozatier and Vincent, 1999)

Mutagen

in vitro construct

(Crozatier and Vincent, 1999)

Progenitor genotype

Carried in construct

P{col5-cDNA.C}

Cytology

Description

Expression of kn cDNA is governed by 5kb of 5' kn sequences.

(Crozatier and Vincent, 1999)

Allele components

Component

Use(s)

Regulatory region(s)

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with kn^col-1

(Crozatier et al., 2004)

visible

(Vervoort et al., 1999)

visible, with kn^col-1

(Crozatier et al., 2002)

wild-type

(Crozatier and Vincent, 1999, Crozatier et al., 1999)

Phenotype Manifest In

anterior crossvein

(Vervoort et al., 1999)

first posterior wing cell

(Vervoort et al., 1999)

first posterior wing cell, with kn^col-1

(Crozatier et al., 2002)

posterior crossvein

(Vervoort et al., 1999)

second posterior wing cell, with kn^col-1

(Crozatier et al., 2002)

submarginal wing cell, with kn^col-1

(Crozatier et al., 2002)

third posterior wing cell, with kn^col-1

(Crozatier et al., 2002)

wing, with kn^col-1

(Crozatier et al., 2002)

wing | P-stage, with kn^col-1

(Crozatier et al., 2002)

wing vein

(Vervoort et al., 1999)

wing vein L3

(Vervoort et al., 1999)

wing vein L3, with kn^col-1

(Crozatier et al., 2002)

wing vein L4

(Vervoort et al., 1999)

wing vein L4, with kn^col-1

(Crozatier et al., 2002)

Detailed Description

Statement

Reference

kn^col-1 kn^+m5 larvae show melanisation of crystal cells after heating at 65[o]C for 10 minutes, as occurs in wild-type larvae.

(Dudzic et al., 2015)

In kn^col-1 homozygotes and kn^col-1/Df(2R)AN293 mutant animals rescued to adult viability by kn^+m5 do have plasmatocytes and crystal cells as normal, however when exposed to wasp parasitization a complete lack of lamellocytes is seen (in contrast to the increased numbers seen in wild-type).

(Crozatier et al., 2004)

kn^col-1 homozygotes (rescued to adult viability by kn^+m5) lack the L3-L4 intervein in the wing. A central larger L3-type vein is present in these flies and only a small, proximal region of wing vein L4 forms. There is a 20% overall reduction in wing size compared to wild type. The L2-L3 intervein is 12+/-5% larger than in wild type. The L4-L5 and L5 to posterior margin interveins are smaller than wild type. The wave of mitosis that normally takes place in each intervein primordium between 15 and 21 hours after puparium formation is specifically absent from the central region of the wings of kn^col-1 animals (rescued with kn^+m5).

(Crozatier et al., 2002)

When used to partially rescue the homozygous kn^col-1 phenotype, a wing vein phenotype remains. The L3-L4 intervein or anterior crossvein is completely absent, the overall size of the wing and the number of innervated bristles at the anterior margin are unchanged. Wing veins L3 and L4 are apposed and abnormally large, each vein maintaining its identity. The number, ratio and position along the A-P axis of socketed and unsocketed bristles is normal.

(Vervoort et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

kn^+m5, kn^col-1 has wing vein L4 phenotype, non-suppressible by vn^UAS.cSa/Scer\GAL4^dpp.blk1

(Crozatier et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of vn^Scer\UAS.cSa under the control of Scer\GAL4^dpp.blk1 does not rescue wing vein L4 formation in kn^col-1 animals rescued with kn^+m5, while the larger L3-type vein seen in these animals is widened.

(Crozatier et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

kn^+m5 rescues kn^col-2

(Crozatier and Vincent, 1999)

kn^+m5 rescues kn^col-3

(Crozatier and Vincent, 1999)

kn^+m5 rescues kn^col-1

(Crozatier and Vincent, 1999)

kn^+m5 rescues kn^col-2

(Crozatier et al., 1999)

kn^+m5 rescues kn^col-3

(Crozatier et al., 1999)

kn^+m5 rescues kn^col-1

(Crozatier et al., 1999)

Partially rescues

kn^+m5 partially rescues kn^col-1

(Vervoort et al., 1999)

Comments

Rescues the embryonic/larval lethality of kn^col-1, kn^col-2 and kn^col-3 embryos; about 80% of the rescued embryos hatch into wandering larvae and reach adulthood. Complete rescue of the head skeleton defects is seen in the cuticles of such embryos.

(Crozatier et al., 1999)

Rescues the lethality of mutants homozygous for kn^col-1, but retains a wing vein phenotype.

(Vervoort et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

col^+m5

kn^+m5

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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