FlyBase Allele Report: Dmel\arm[UAS.Tag:MYC,Tag:NLS(SV40-largeT)]

General Information

Symbol

Dmel\arm^{UAS.Tag:MYC,Tag:NLS(SV40-largeT)}

Species

D. melanogaster

Name

FlyBase ID

FBal0097064

Feature type

allele

Associated gene

Dmel\arm

Associated Insertion(s)

Carried in Construct

P{UAS-arm.NLS}

Key Links

Transgenic product class

wild_type

(Cox et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Cox et al., 1999)

Mutagen

in vitro construct

(Cox et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-arm.NLS}

Cytology

Description

UASt regulatory sequences drive expression of arm tagged at the N-terminus with Tag:NLS(SV40-largeT) (a nuclear localisation signal) and tagged at the C-terminus with 5 copies of Tag:MYC.

(Cox et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tagged with

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/first instar larval cuticle, with Scer\GAL4^da.G32

(Townsley et al., 2004)

embryonic/first instar larval cuticle, with Scer\GAL4^e22c

(Cox et al., 1999)

ventral denticle belt, with Scer\GAL4^e22c

(Cox et al., 1999)

Detailed Description

Statement

Reference

Overexpression of arm^{Scer\UAS.T:Hsap\MYC,T:SV40\nls2}, under the regulation of Scer\GAL4^da.G32 produces patches of naked cuticle along the ventral midline.

(Townsley et al., 2004)

Embryos expressing arm^{Scer\UAS.T:Hsap\MYC,T:SV40\nls2} under the control of Scer\GAL4^e22c show a partial loss of denticle belts and their replacement by naked cuticle.

(Cox et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^da.G32/arm^{UAS.Tag:MYC,Tag:NLS(SV40-largeT)} is a suppressor of embryonic/first instar larval cuticle | maternal effect phenotype of lgs^20F

(Townsley et al., 2004)

Scer\GAL4^da.G32/arm^{UAS.Tag:MYC,Tag:NLS(SV40-largeT)} is a suppressor of embryonic/first instar larval cuticle | maternal effect phenotype of pygo^S123

(Townsley et al., 2004)

Scer\GAL4^da.G32/arm^{UAS.Tag:MYC,Tag:NLS(SV40-largeT)} is a suppressor of embryonic/first instar larval cuticle | maternal effect phenotype of pygo^S28

(Townsley et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of arm^{Scer\UAS.T:Hsap\MYC,T:SV40\nls2}, under the regulation of Scer\GAL4^da.G32 suppresses the mutant phenotype of pygo^S28 embryos which lack both maternal and zygotic pygo function. The degree of suppression varies very little between individual embryos, and is essentially the same as that provided by zygotic pygo expressed from the paternal wild-type allele. Overexpression of arm^{Scer\UAS.T:Hsap\MYC,T:SV40\nls2}, under the regulation of Scer\GAL4^da.G32 in lgs^20F mutants which lack both maternal and zygotic lgs function converts the lgs^20F denticle lawn into an entirely naked cuticle along the ventral midline. Overexpression of arm^{Scer\UAS.T:Hsap\MYC,T:SV40\nls2}, under the regulation of Scer\GAL4^da.G32 suppresses the mutant phenotype of pygo^S123 embryos which lack both maternal and zygotic pygo function. The degree of suppression varies very little between individual embryos, and is essentially the same as that provided by zygotic pygo expressed from the paternal wild-type allele.

(Townsley et al., 2004)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

arm^{UAS.Tag:MYC,Tag:NLS(SV40-largeT)}/Scer\GAL4^e22c partially rescues arm²

(Cox et al., 1999)

Comments

The segment polarity phenotype of embryos maternally and zygotically mutant for arm² is substantially rescued by arm^{Scer\UAS.T:Hsap\MYC,T:SV40\nls2} expressed under the control of Scer\GAL4^e22c.

(Cox et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

arm^{Scer\UAS.T:Hsap\MYC,T:SV40\nls-largeT}

arm^{Scer\UAS.T:Hsap\MYC,T:SV40\nls2}

arm^{UAS.Tag:MYC,Tag:NLS(SV40-largeT)}

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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