FlyBase Allele Report: Dmel\GluRIIB[UAS.cDa]

General Information

Symbol

Dmel\GluRIIB^UAS.cDa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of DiAntonio

FlyBase ID

FBal0097261

Feature type

allele

Associated gene

Dmel\GluRIIB

Associated Insertion(s)

Carried in Construct

P{UAS-GluRIIB.D}

Key Links

Transgenic product class

cDNA

(DiAntonio et al., 1999)

wild_type

(DiAntonio et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(DiAntonio et al., 1999)

wild_type

(DiAntonio et al., 1999)

Mutagen

in vitro construct

(DiAntonio et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-GluRIIB.D}

Cytology

Description

UASt regulatory sequences drive expression of a GluRIIB cDNA.

(DiAntonio et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

GluRIIB

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^Mhc.PW

(Sigrist et al., 2002)

Phenotype Manifest In

neuromuscular junction, with Scer\GAL4^how-24B

(DiAntonio et al., 1999)

neuromuscular junction, with Scer\GAL4^Mhc.PW

(DiAntonio et al., 1999)

neuromuscular junction | larval stage, with Scer\GAL4^Mhc.PW

(Sigrist et al., 2002)

Detailed Description

Statement

Reference

Overexpression of GluRIIB^Scer\UAS.cDa, under the regulation of Scer\GAL4^Mhc.PW does not result in significant alterations of the morphological development of larval stage larval stage neuromuscular junctions compared with controls.

(Sigrist et al., 2002)

The quantal size at the neuromuscular junction is larger in Df(2L)SP22 larvae rescued with GluRIIA^Scer\UAS.cPa expressed under the control of Scer\GAL4^how-24B than in Df(2L)SP22 larvae rescued with GluRIIB^Scer\UAS.cDa expressed under the control of Scer\GAL4^how-24B. There is a significant reduction in the quantal size at the neuromuscular junction in larvae expressing GluRIIB^Scer\UAS.cDa under the control of Scer\GAL4^how-24B or Scer\GAL4^Mhc.PW compared to wild type.

(DiAntonio et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

GluRIIB^UAS.cDa, Scer\GAL4^Mhc.PW has abnormal neuroanatomy phenotype, suppressible by GluRIIA^UAS.cPa, Scer\GAL4^Mhc.PW

(Sigrist et al., 2002)

NOT Suppressor of

Statement

Reference

GluRIIB^UAS.cDa, Scer\GAL4^Mhc.PW is a non-suppressor of abnormal neuroanatomy phenotype of GluRIIA^UAS.cPa, Scer\GAL4^Mhc.PW

(Sigrist et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

GluRIIB^UAS.cDa, Scer\GAL4^Mhc.PW has bouton phenotype, suppressible by GluRIIA^UAS.cPa, Scer\GAL4^Mhc.PW

(Sigrist et al., 2002)

NOT Suppressor of

Statement

Reference

GluRIIB^UAS.cDa, Scer\GAL4^Mhc.PW is a non-suppressor of synapse phenotype of GluRIIA^UAS.cPa, Scer\GAL4^Mhc.PW

(Sigrist et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Overexpression of both GluRIIB^Scer\UAS.cDa and GluRIIA^Scer\UAS.cPa, under the regulation of Scer\GAL4^Mhc.PW significantly suppresses the synaptic bouton addition observed in GluRIIA^Scer\UAS.cPa Scer\GAL4^Mhc.PW animals. Simultaneous transgenic overexpression of GluRIIA^Scer\UAS.cPa and GluRIIB^Scer\UAS.cDa, under the regulation of Scer\GAL4^Mhc.PW does not result in an increased synaptic accumulation of GluRIIA subunits. Neuromuscular junctions with unaltered synaptic GluRIIA levels exhibit a wild-type number of junctional boutons.

(Sigrist et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

64257

w^*; P{UAS-GluRIIB.D}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Glu-RIIB^Scer\UAS.cDa

GluRIIB^Scer\UAS.cDa

GluRIIB^UAS.cDa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of DiAntonio

(DiAntonio et al., 1999)

Secondary FlyBase IDs

References (3)

Report Sections

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