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General Information
Symbol
Dmel\Pka-C1UAS.Tag:FLAG
Species
D. melanogaster
Name
FlyBase ID
FBal0098329
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Pka-C1, UAS-PKAcF, UAS-DC0, UAS-DC0+, UAS-PKA, UAS-PKA-C1-Flag, UAS-PKA-C
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of wild type genomic Pka-C1. Also tagged with Tag:FLAG at C terminus.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Larval fat body clones expressing Pka-C1UAS.Tag:FLAG under the control of Scer\GAL4Act.PU do not show significant changes in autophagosome induction in response to starvation.

Overexpressing Pka-C1Scer\UAS.T:Zzzz\FLAG using Scer\GAL4c747 disrupts 1hr memory in young flies.

Expression of Pka-C1Scer\UAS.T:Zzzz\FLAG using a mushroom body driver (Scer\GAL4c309) abolishes 24-h memory after massed training in both wild-type and a Pka-C1B3/+ heterozygous background.

Expression of Pka-C1Scer\UAS.T:Zzzz\FLAG driven by Scer\GAL4Tab2-201Y has does not effect anesthesia-resistant memory nor long-term memory in a wild-type background.

Overexpression of Scer\GAL4c819 driven by Scer\GAL4c819 does not boost memory.

Expression of insertion P{UAS-Pka-C1.FLAG}5.5 in Scer\GAL4RK5 background allows partial survival; 10 with a sibling class of 81. Expression of insertion P{UAS-Pka-C1.FLAG}5.9 in Scer\GAL4RK5 background allows complete survival; 52 with a sibling class of 48. Those that survive to adulthood show a range of phenotypes, including small size, warped or collapsed wings, flecks on the surface of the eye, bristles glued to the thorax. Some show a combination of these defects, others were normal. Both P{UAS-Pka-C1.FLAG}5.5 and 5.9 show complete lethality in backgrounds Scer\GAL4JW1, Scer\GAL4PP3, Scer\GAL4KO5. Insertions P{UAS-Pka-C1.FLAG}1.1, 1.3, 5.2 show complete lethality in all of the backgrounds Scer\GAL4JW1, Scer\GAL4PP3, Scer\GAL4KO5, and Scer\GAL4RK5. Expression of insertion P{UAS-Pka-C1.FLAG}5.9 in Scer\GAL4RZ4 background produces adults 51% of which have notched wing margins.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Enhancer of
Suppressor of
Statement
Reference

Pka-C1UAS.Tag:FLAG, Scer\GAL4e22c is a suppressor | partially of denticle belt & thoracic segment phenotype of Pka-R1BDK.UAS, Scer\GAL4e22c

Pka-C1UAS.Tag:FLAG, Scer\GAL4e22c is a suppressor of denticle belt & abdominal segment phenotype of Pka-R1BDK.UAS, Scer\GAL4e22c

Additional Comments
Genetic Interactions
Statement
Reference

PCBEP2547/+ suppresses 1hr memory impairment caused by overexpressing Pka-C1Scer\UAS.T:Zzzz\FLAG using Scer\GAL4c747.

Coexpression of Pka-R1BDK.Scer\UAS.T:Ivir\HA1 and Pka-C1Scer\UAS.T:Zzzz\FLAG, both under the control of Scer\GAL4Tab2-201Y, suppresses the reduced ethanol sensitivity phenotype of Pka-R1BDK.Scer\UAS.T:Ivir\HA1.

The P-Repressor encoded by P{P-Sal}89D has been used to suppress the lethality caused by expression of Pka-C1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL471B. This allows mosaic analysis of the phenotype caused by expression of Pka-C1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL471B; almost half the mosaic flies have ectopic wing veins located between veins L3 and L4 in otherwise normal wings.

Coexpression of P{UAS-Pka-R1.BDK} and P{UAS-Pka-C1.FLAG} in a Scer\GAL4e22c background rescues the abdominal denticle belt phenotype of P{UAS-Pka-R1.BDK}, and partially restores thoracic denticle belts.

Xenogenetic Interactions
Statement
Reference

Co-expression of Pka-C1Scer\UAS.T:Zzzz\FLAG significantly rescues the short term memory defects of flies expressing Hsap\APP695.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4c739.

Co-expression of Pka-C1Scer\UAS.T:Zzzz\FLAG strongly enhances the rough eye phenotype caused by expression of Hsap\MAPTV337M.Scer\UAS under the control of Scer\GAL4elav.PLu.

The lethality caused by Ocun\PKIScer\UAS.T:Zzzz\FLAG or Pka-C1Scer\UAS.T:Zzzz\FLAG expressed under the control of Scer\GAL4RK5 is suppressed if they are coexpressed under the control of Scer\GAL4RK5.

Expression of Ocun\PKIScer\UAS.T:Zzzz\FLAG suppresses the wing phenotype caused by expression of Pka-C1Scer\UAS.T:Zzzz\FLAG under the control of Scer\GAL4RZ4.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Pka-C1Scer\UAS.T:Zzzz\FLAG
Pka-C1UAS.Tag:FLAG
Name Synonyms
Secondary FlyBase IDs
    References (19)