FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Syt1UAS.cLa
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General Information
Symbol
Dmel\Syt1UAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Littleton
FlyBase ID
FBal0099815
Feature type
allele
Associated gene
Dmel\Syt1
Associated Insertion(s)
Carried in Construct
P{UAS-sytI.L}
Also Known As
UAS-synaptotagmin I
Key Links
Transgenic product class
wild_type
(Littleton et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Littleton et al., 1999)
Mutagen
in vitro construct
(Littleton et al., 1999)
Progenitor genotype
Carried in construct
P{UAS-sytI.L}
Cytology
Description

A 2kb fragment encompassing the Syt1 ORF is driven by UASt sequences.

(Littleton et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
Syt1
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of sytScer\UAS.cLa under the control of Scer\GAL44G results in a significant increase in FM1-43 dye uptake at the neuromuscular junction after stimulus compared to controls.

      (Fergestad and Broadie, 2001)

      Overexpression of sytScer\UAS.cLa in neurons, driven by Scer\GAL4elav.PLu creates larvae that when tested electrophysiologically by stimulation of the neuromuscular junction, produce peak amplitudes of the excitatory junction potential that are not significantly different from wild-type. No decrease in muscle miniature excitatory junction potential (MEJP) amplitude or muscle resting membrane potential is seen in sytScer\UAS.cLa,Scer\GAL4elav.PLu larvae, however MEJP frequency is decreased by about a third.

      (Littleton et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The lethality of stnA1 stnB1/Y ("stn8P1") animals is not rescued by expression of sytScer\UAS.cLa under the control of either Scer\GAL4elav-C155 or Scer\GAL4shi.PS. The lethality of stnA12 stnB12/Y ("stn13-120") animals is not rescued by expression of sytScer\UAS.cLa under the control of either Scer\GAL4elav-C155 or Scer\GAL4shi.PS.

      (Estes et al., 2003)

      stnA12 stnB12 homozygotes show embryonic lethality. Expression of sytScer\UAS.cLa under the control of Scer\GAL41407 in this background results in approximately 98% of animals hatching at normal times, but they then die as first instar larvae. Expression of sytScer\UAS.cLa under the control of Scer\GAL44G in the stnA12 stnB12 background results in approximately 96% of embryos hatching, although hatching is delayed (animals hatch between 21 and 35 hours after fertilisation. These animals show adult viability. Expression of sytScer\UAS.cLa under the control of Scer\GAL44G rescues the endocytotic functional defects seen in stnA15 stnB15 ("stnC") mutants.

      (Fergestad and Broadie, 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Syt1UAS.cLa/Scer\GAL4elav.PLu rescues Syt1AD4/Syt1N13

      (Yoshihara et al., 2010)

      Scer\GAL4elav-C155/Syt1UAS.cLa rescues Syt1AD4/Syt1N13

      (Adolfsen et al., 2004)
      Comments

      Expression of Syt1Scer\UAS.cLa under the control of Scer\GAL4elav.PLu completely rescues the defects in the evoked synaptic currents recorded from muscle 6 of Syt1N13/Syt1AD4 embryos following motor nerve stimulation in 4mM extracellular Ca[2+]; evoked release is fully synchronous, and few asynchronous release events are seen in the rescued animals.

      (Yoshihara et al., 2010)

      Transgenic expression of sytScer\UAS.cLa (under the regulation of Scer\GAL4elav-C155) in the null sytAD4/sytN13 background is able to partially restore all the behavioral defects observed. Overexpression of sytScer\UAS.cLa (under the regulation of Scer\GAL4elav-C155) is able to restore evoked excitatory junctional potential (EJP) amplitudes to near wild-type levels in sytAD4/sytN13 mutants.

      (Adolfsen et al., 2004)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Syt1Scer\UAS.cLa
      Syt1UAS.cLa
      sytScer\UAS.cLa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Littleton
      (Littleton et al., 1999)
      Secondary FlyBase IDs
        References (6)