FlyBase Allele Report: Dmel\stnA[12]

General Information

Symbol

Dmel\stnA¹²

Species

D. melanogaster

Name

FlyBase ID

FBal0016212

Feature type

allele

Associated gene

Dmel\stnA

Associated Insertion(s)

Doc{}stnA¹²

Carried in Construct

Also Known As

stn^13-120

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

spontaneous

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Petrovich et al., 1993)

Mutagen

spontaneous

(Schalet, 1986)

Progenitor genotype

Associated Insertion(s)

Doc{}stnA¹²

Cytology

Description

Insertion into ORF1.

(Andrews et al., 1996)

Doc element insertion.

(Andrews et al., 1995)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon terminus

(Fergestad et al., 1999)

multivesicular body

(Fergestad et al., 1999)

synaptic vesicle

(Fergestad et al., 1999)

Detailed Description

Statement

Reference

Mutants die as mature embryos after a failure to hatch, due to lack of coordination. Mutant embryos show normal segmental patterning of the epidermis, muscles and nervous system. Mutant nmjs appear morphologically wild type though the nerve terminals are slightly smaller than normal. In electrophysiological recordings at the embryonic nmj nerve stimulation produces muscle contraction demonstrating that presynaptic depolarization evokes transmitter release and that muscle excitation-secretion response is intact. However evoked EJC peak amplitudes are significantly reduced and the release of neurotransmitter at mutant synapses is markedly asynchronous due to delayed presynaptic vesicle fusion. Mutants have an impaired ability to synchronously trigger calcium-mediated vesicle fusion. The overall level of neurotransmitter release is reduced. Variability in EJC peak amplitudes is increased, compared to wild type. Transmission fidelity is lost. Calcium sensitivity is unaltered. MEJC frequency is not significantly altered, though amplitude is increased, perhaps because of increased quantity of neurotransmitter in some vesicles. Mutant synapses exhibit severe fatigue after prolonged stimulation. Synapses show decreased synaptic vesicle density and accumulate membrane-recycling intermediates.

(Fergestad et al., 1999)

Flies heterozygous for stnA¹⁵ and any of the lethal "stn" alleles show either a small or no off transient in ERGs. Flies heterozygous for a ts and a lethal allele of stnA show ERGs with off transient amplitudes between those for stnA⁷ and stnA¹⁵.

(Petrovich et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

stnA¹², stnB¹² has lethal | recessive | embryonic stage phenotype, suppressible by Scer\GAL4^insc-Mz1407/Syt1^UAS.cLa

(Fergestad and Broadie, 2001)

stnA¹², stnB¹² has lethal | recessive | embryonic stage phenotype, suppressible by Scer\GAL4^4G/Syt1^UAS.cLa

(Fergestad and Broadie, 2001)

NOT suppressed by

Statement

Reference

stnA¹² has lethal | recessive phenotype, non-suppressible by Su(stn)¹

(Petrovich et al., 1993)

Other

Statement

Reference

stnA¹², stnB¹² has lethal phenotype

(Estes et al., 2003)

Scer\GAL4^shi.PS, stnA¹², stnA^AB.UAS, stnB¹², stnB^AB.UAS has viable phenotype

(Estes et al., 2003)

Scer\GAL4^elav-C155, stnA¹², stnA^AB.UAS, stnB¹², stnB^AB.UAS has viable phenotype

(Estes et al., 2003)

Scer\GAL4^elav-C155, stnA¹², stnB¹², stnB^AB.UAS has viable phenotype

(Estes et al., 2003)

Scer\GAL4^Mhc.PW, stnA¹², stnA^AB.UAS, stnB¹², stnB^AB.UAS has lethal phenotype

(Estes et al., 2003)

Scer\GAL4^Mhc.PW, stnA¹², stnB¹², stnB^UAS.cEa has lethal phenotype

(Estes et al., 2003)

Scer\GAL4^elav-C155, stnA¹², stnA^UAS.cEa, stnB¹² has lethal phenotype

(Estes et al., 2003)

stnA¹², stnB¹² has lethal | recessive | embryonic stage phenotype

(Fergestad and Broadie, 2001)

Scer\GAL4^insc-Mz1407, Syt1^UAS.cLa, stnA¹², stnB¹² has lethal | larval stage phenotype

(Fergestad and Broadie, 2001)

Scer\GAL4^4G, Syt1^UAS.cLa, stnA¹², stnB¹² has viable phenotype

(Fergestad and Broadie, 2001)

stnA¹²/stnA¹, stnB¹/stnB¹² has lethal phenotype

(Stimson et al., 2001)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

stnA¹², stnB⁶, stnA⁶, stnB¹² is a non-enhancer of neuromuscular junction phenotype of shi²

(Stimson et al., 2001)

Other

Statement

Reference

stnA¹²/stnA⁶, stnB⁶/stnB¹² has neuromuscular junction & synaptic vesicle phenotype

(Stimson et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

stnA¹² stnB¹²/Y ("stn^13-120") males do not survive to adulthood. The lethality of stnA¹² stnB¹²/Y ("stn^13-120") animals is not rescued by expression of syt^Scer\UAS.cLa under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^shi.PS.

(Estes et al., 2003)

stnA¹² stnB¹² ("stn^13-120") mutant embryos show a more than 90% reduction in FM1-43 dye uptake at the neuromuscular junction after stimulus compared to wild type. stnA¹² stnB¹² ("stn^13-120") homozygotes show embryonic lethality. Expression of syt^Scer\UAS.cLa under the control of Scer\GAL4¹⁴⁰⁷ in this background results in approximately 98% of animals hatching at normal times, but they then die as first instar larvae. Expression of syt^Scer\UAS.cLa under the control of Scer\GAL4^4G in the stnA¹² stnB¹² background results in approximately 96% of embryos hatching, although hatching is delayed (animals hatch between 21 and 35 hours after fertilisation. These animals show adult viability.

(Fergestad and Broadie, 2001)

Synaptic vesicle internalisation at the neuromuscular junction is delayed relative to wild type in stnB⁶/stnB¹² mutants.

(Stimson et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

stnA¹⁵

(Stimson et al., 1998)

stnA⁷

(Stimson et al., 1998)

Comments

Expression of the dicistronic P{UAS-stnAB.E} construct (which expresses stnA^AB.Scer\UAS and stnB^AB.Scer\UAS) under the control of either Scer\GAL4^elav-C155 or Scer\GAL4^shi.PS rescues the lethality of stnA¹² stnB¹²/Y ("stn^13-120") animals. Expression of the dicistronic P{UAS-stnAB.E} construct (which expresses stnA^AB.Scer\UAS and stnB^AB.Scer\UAS) under the control of Scer\GAL4^Mhc.PW does not rescue the lethality of stnA¹² stnB¹²/Y ("stn^13-120") animals. Expression of stnB^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 rescues the lethality of stnA¹² stnB¹²/Y ("stn^13-120") animals. Expression of stnB^Scer\UAS.cEa under the control of Scer\GAL4^Mhc.PW does not rescue the lethality of stnA¹² stnB¹²/Y ("stn^13-120") animals. Expression of stnA^Scer\UAS.cEa under the control of Scer\GAL4^elav-C155 does not rescue the lethality of stnA¹² stnB¹²/Y ("stn^13-120") animals.

(Estes et al., 2003)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Schalet.

Induced with: stnB¹².

(Fergestad et al., 1999)

Comments

Used in mosaic analysis to show that the "stn" product is not required for all cell types, but is required in the head for normal active behavior and in the thorax for motor coordination and posture.

(Petrovich et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

l(1)13-120

(Schalet, 1986)

stn13-120

(Fergestad and Broadie, 1997)

stn¹²

stn^13-120

(Wall et al., 2005, Estes et al., 2003, Fergestad and Broadie, 2001, Stimson et al., 2001, Fergestad et al., 1999, Stimson et al., 1998, Andrews et al., 1996, Andrews et al., 1995, Petrovich et al., 1993)

stnA¹²

Name Synonyms

Secondary FlyBase IDs

References (11)

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